Construct: ORF TRCN0000489329
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020214.1_s317c1
- DNA Barcode:
- GGCCTGCTGGTGCCCCCTTCCTGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PI4K2A (55361)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489329
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55361 | PI4K2A | phosphatidylinositol 4-kina... | NM_018425.3 | 100% | 100% | |
| 2 | mouse | 84095 | Pi4k2a | phosphatidylinositol 4-kina... | NM_145501.2 | 91.9% | 95.4% | (many diffs) |
| 3 | mouse | 84095 | Pi4k2a | phosphatidylinositol 4-kina... | XM_011247405.2 | 91.9% | 95.4% | (many diffs) |
| 4 | mouse | 84095 | Pi4k2a | phosphatidylinositol 4-kina... | XM_011247406.2 | 88.1% | 91.4% | (many diffs) |
| 5 | mouse | 84095 | Pi4k2a | phosphatidylinositol 4-kina... | XR_877696.2 | 57.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1509
- ORF length:
- 1437
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggacgag acgagcccac tagtgtcccc cgagcgggcc caacccccgg 121 actacacctt cccgtcgggc tcgggcgctc actttccgca ggtgcccggg ggcgcggtcc 181 gagtggcggc ggcggccggc tcgggcccct ctccgccggg ctcgccgggc cacgaccgcg 241 agcggcagcc actgttggat cgggcccggg gcgcggcggc ccagggccag acccaaaccg 301 tggcggcgca ggcccaggct ctggccgctc aggccgcggc ggcagcccac gccgctcagg 361 cccaccgcga gcggaacgag ttcccggagg atcctgagtt cgaggcggtg gtgcggcagg 421 ccgagctggc catcgagcgc tgcatctttc ccgagcgcat ctaccagggc tccagcggaa 481 gctacttcgt caaggaccct caggggagga tcattgctgt cttcaaaccc aagaatgaag 541 agccctatgg gcatcttaat cctaagtgga ccaagtggct gcagaagctg tgctgtcctt 601 gctgctttgg ccgtgactgc cttgtcctta accagggcta tctctcagaa gcaggggcca 661 gcctggtgga ccaaaaactg gaactcaaca ttgttccccg tacaaaggta gtatacctgg 721 ccagtgagac cttcaactat agtgccattg accgagtgaa gtccaggggc aagcggcttg 781 cactagagaa agtgccaaaa gttggacagc ggtttaaccg catcgggcta ccaccaaagg 841 ttggttcatt ccagctcttt gttgaaggct acaaagatgc agactattgg ctgcggcgtt 901 ttgaagcaga acctcttcct gagaacacta accggcaact actgctccag tttgagcggt 961 tggtggtgct ggattacatc atccgcaaca ctgatcgagg caatgacaac tggctgatta 1021 aatatgactg tccaatggat agttctagct ctcgggacac agactgggtg gtggtgaagg 1081 agcctgttat caaggtggct gccatagaca atgggctggc cttcccactg aagcatcctg 1141 actcctggag ggcatatcct ttttactggg cctggttgcc cCAGGCGAAA GTCCCATTTT 1201 CTCAGGAGAT CAAAGATCTG ATCCTTCCAA AGATATCGGA CCCTAACTTC GTCAAGGACT 1261 TGGAAGAGGA CCTATATGAA CTCTTCAAGA AAGATCCTGG TTTCGACAGG GGCCAGTTCC 1321 ATAAGCAGAT TGCTGTCATG CGGGGCCAGA TCTTAAATCT GACCCAGGCC TTGAAAGACA 1381 ACAAGAGTCC CCTGCACCTC GTCCAGATGC CACCTGTGAT TGTCGAGACG GCCCGTTCCC 1441 ACCAGCGGTC TTCTAGCGAG TCCTACACAC AGAGCTTTCA GAGCCGGAAG CCCTTCTTTT 1501 CATGGTGGTG AGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1561 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1621 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGGC CTGCTGGTGC CCCCTTCCTG 1681 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t