Construct: ORF TRCN0000489333
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019268.1_s317c1
- DNA Barcode:
- TCGACCAGCTTGAAATACATCAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WASF2 (10163)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489333
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10163 | WASF2 | WASP family member 2 | NM_006990.5 | 99.9% | 100% | 1239G>A |
| 2 | human | 10163 | WASF2 | WASP family member 2 | NM_001201404.3 | 55.8% | 55% | (many diffs) |
| 3 | mouse | 242687 | Wasf2 | WAS protein family, member 2 | NM_153423.6 | 87.6% | 91.2% | (many diffs) |
| 4 | mouse | 242687 | Wasf2 | WAS protein family, member 2 | XM_006538848.3 | 87.6% | 91.2% | (many diffs) |
| 5 | mouse | 242687 | Wasf2 | WAS protein family, member 2 | XM_006538849.3 | 87.6% | 91.2% | (many diffs) |
| 6 | mouse | 242687 | Wasf2 | WAS protein family, member 2 | XM_006538850.3 | 87.6% | 91.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1569
- ORF length:
- 1494
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgccg ttagtaacga ggaacatcga gccaaggcac ctgtgccgtc 121 agacgttgcc tagcgttaga agcgagctgg aatgcgtgac caacatcacc ctggcaaatg 181 tcatccgaca gctgggcagc ctgagtaaat atgcagagga catttttgga gagctcttta 241 ctcaggcaaa tacctttgcc tctcgggtaa gctcccttgc tgagagggtc gaccgactac 301 aggttaaagt cactcagctg gatcccaagg aagaagaagt gtcactgcaa ggaatcaaca 361 cccgaaaagc cttcagaagt tccaccattc aagaccagaa gctttttgac agaaactctc 421 tcccagtgcc tgtcttagaa acatacaata cctgtgatac tcctccccct ctcaacaatc 481 ttacccctta cagggacgat ggaaaagagg cactcaaatt ctacacagac ccttcatact 541 tctttgatct ttggaaggag aagatgctgc aggacaccaa ggatatcatg aaagagaaga 601 gaaagcatag gaaagaaaag aaagataatc caaatcgagg gaatgtaaac ccacgtaaaa 661 tcaagacacg taaggaagag tgggagaaaa tgaagatggg gcaagaattt gtggagtcca 721 aagaaaagct ggggacttct gggtatccac ccactttggt gtaccagaat ggcagcattg 781 gctgtgttga aaacgtggat gcaagtagct atccgccacc accacagtca gactctgctt 841 cttcaccttc tccttccttc tccgaggaca acttgcctcc tccaccagca gaattcagtt 901 acccagtgga caaccaaaga ggatctggtt tggctggacc caaaagatcc agtgtggtca 961 gcccaagcca tccaccacca gctcctcctc taggctctcc accaggccct aaacccgggt 1021 ttgctccacc acctgcccct ccgccacctc cgcctccaat gataggcatc ccacctccac 1081 caccgcctgt aggatttggg tctccaggga cgcctccacc accctcaccc ccatctttcc 1141 cacctcaccc tgattttgct gcccctccac ctcctcctcc accaccagca gctgactacc 1201 caactctgcc accacctccc ttgtcccagc caacaggagg agcacctcct cctccccctc 1261 ctcctcctcc TCCGGGGCCC CCTCCTCCCC CTTTCACTGG TGCAGATGGC CAACCTGCTA 1321 TACCACCACC GCTTTCTGAT ACCACCAAGC CCAAGTCCTC CTTGCCTGCC GTGAGCGATG 1381 CCCGTAGCGA CCTGCTTTCA GCCATCCGTC AAGGTTTTCA GCTGCGCAGG GTTGAGGAGC 1441 AGCGGGAACA AGAGAAGCGG GATGTTGTGG GCAATGACGT GGCCACCATC TTGTCTCGTC 1501 GCATTGCTGT TGAGTACAGT GACTCAGAAG ATGACTCCTC TGAATTTGAT GAGGACGACT 1561 GGTCCGATGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1621 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1681 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATCGACC AGCTTGAAAT ACATCAACAC 1741 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt