Construct: ORF TRCN0000489335
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019153.1_s317c1
- DNA Barcode:
- CAGATAGACCCAGCGGTGCGCTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTH2R (5746)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489335
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NM_005048.4 | 100% | 100% | |
| 2 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NM_001309516.2 | 79.8% | 79.8% | 0_1ins333 |
| 3 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NM_001371905.1 | 79.8% | 79.8% | 0_1ins333 |
| 4 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NM_001371906.1 | 79.8% | 79.8% | 0_1ins333 |
| 5 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NM_001371907.1 | 63% | 63% | 0_1ins333;647_648ins276 |
| 6 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NR_163993.1 | 53.1% | (many diffs) | |
| 7 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NR_163994.1 | 52.6% | (many diffs) | |
| 8 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NR_163991.1 | 51.8% | (many diffs) | |
| 9 | human | 5746 | PTH2R | parathyroid hormone 2 receptor | NR_163992.1 | 50.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1725
- ORF length:
- 1650
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggcc gggctggggg cgtcgctcca cgtctggggt tggctaatgc 121 tcggcagctg cctcctggcc agagcccagc tggattctga tggcaccatt actatagagg 181 agcagattgt ccttgtgctg aaagcgaaag tacaatgtga actcaacatc acagctcaac 241 tccaggaggg agaaggtaat tgtttccctg aatgggatgg actcatttgt tggcccagag 301 gaacagtggg gaaaatatcg gctgttccat gccctcctta tatttatgac ttcaaccata 361 aaggagttgc tttccgacac tgtaacccca atggaacatg ggattttatg cacagcttaa 421 ataaaacatg ggccaattat tcagactgcc ttcgctttct gcagccagat atcagcatag 481 gaaagcaaga attctttgaa cgcctctatg taatgtatac cgttggctac tccatctctt 541 ttggttcctt ggctgtggct attctcatca ttggttactt cagacgattg cattgcacta 601 ggaactatat ccacatgcac ttatttgtgt ctttcatgct gagagctaca agcatctttg 661 tcaaagacag agtagtccat gctcacatag gagtaaagga gctggagtcc ctaataatgc 721 aggatgaccc acaaaattcc attgaggcaa cttctgtgga caaatcacaa tatatcgggt 781 gcaagattgc tgttgtgatg tttatttact tcctggctac aaattattat tggatcctgg 841 tggaaggtct ctacctgcat aatctcatct ttgtggcttt cttttcggac accaaatacc 901 tgtggggctt catcttgata ggctgggggt ttccagcagc atttgttgca gcatgggctg 961 tggcacgagc aactctggct gatgcgaggt gctgggaact tagtgctgga gacatcaagt 1021 ggatttatca agcaccgatc ttagcagcta ttgggctgaa ttttattctg tttctgaata 1081 cggttagagt tctagctacc aaaatctggg agaccaatgc agttgggcat gacacaagga 1141 agcaatacag gaaactggcc aaatcgacac tggtcctggt cctagtcttt ggagtgcatt 1201 acatcgtgtt cgtatgcctg cctcactcct tcactgggct cgggtgggag atccgcatgc 1261 actgtgagct cttcttcaac tcctttcagg gtttctttgt gtctatcatc tactgctact 1321 gcaatggaga ggttcaggca gaggtgaaga agatgtggag tcggtggaac ctctccgtgg 1381 actggaaaag gacaccgcca tgtggCAGCC GCAGATGCGG CTCAGTGCTC ACCACCGTGA 1441 CGCACAGCAC CAGCAGCCAG TCACAGGTGG CGGCCAGCAC ACGCATGGTG CTTATCTCTG 1501 GCAAAGCTGC CAAGATCGCC AGCAGACAGC CTGACAGCCA CATCACTTTA CCTGGCTATG 1561 TCTGGAGTAA CTCAGAGCAG GACTGCCTGC CACACTCTTT CCACGAGGAG ACCAAGGAAG 1621 ATAGTGGGAG GCAGGGAGAT GATATTCTAA TGGAGAAGCC TTCCAGGCCT ATGGAATCTA 1681 ACCCAGACAC TGAAGGATGC CAAGGAGAAA CTGAGGATGT TCTCGACCCA GCTTTCTTGT 1741 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1801 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1861 GAAAGGACGA CAGATAGACC CAGCGGTGCG CTATACGCGT TAAGTCgaca atcaacctct 1921 ggattacaaa atttgtgaaa gatt