Construct: ORF TRCN0000489346
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF022038.1_s317c1
- DNA Barcode:
- TCGCCCATACCGGCACTAACCCAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RIPK4 (54101)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489346
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54101 | RIPK4 | receptor interacting serine... | NM_020639.3 | 100% | 100% | |
| 2 | mouse | 72388 | Ripk4 | receptor-interacting serine... | NM_023663.6 | 84.8% | 90.4% | (many diffs) |
| 3 | mouse | 72388 | Ripk4 | receptor-interacting serine... | XM_011246154.2 | 77.6% | 83.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2421
- ORF length:
- 2352
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggagggcgac ggcgggaccc catgggccct ggcgctgctg cgcaccttcg 121 acgcgggcga gttcacgggc tgggagaagg tgggctcggg cggcttcggg caggtgtaca 181 aggtgcgcca tgtccactgg aagacctggc tggccatcaa gtgctcgccc agcctgcacg 241 tcgacgacag ggagcgcatg gagcttttgg aagaagccaa gaagatggag atggccaagt 301 ttcgctacat cctgcctgtg tatggcatct gccgcgaacc tgtcggcctg gtcatggagt 361 acatggagac gggctccctg gaaaagctgc tggcttcgga gccattgcca tgggatctcc 421 ggttccgaat catccacgag acggcggtgg gcatgaactt cctgcactgc atggccccgc 481 cactcctgca cctggacctc aagcccgcga acatcctgct ggatgcccac taccacgtca 541 agatttctga ttttggtctg gccaagtgca acgggctgtc ccactcgcat gacctcagca 601 tggatggcct gtttggcaca atcgcctacc tccctccaga gcgcatcagg gagaagagcc 661 ggctcttcga caccaagcac gatgtataca gctttgcgat cgtcatctgg ggcgtgctca 721 cacagaagaa gccgtttgca gatgagaaga acatcctgca catcatggtg aaggtggtga 781 agggccaccg ccccgagctg ccgcccgtgt gcagagcccg gccgcgcgcc tgcagccacc 841 tgatacgcct catgcagcgg tgctggcagg gggatccgcg agttaggccc accttccaag 901 aaattacttc tgaaaccgag gacctgtgtg aaaagcctga tgacgaagtg aaagaaactg 961 ctcatgatct ggacgtgaaa agccccccgg agcccaggag cgaggtggtg cctgcgaggc 1021 tcaagcgggc ctctgccccc accttcgata acgactacag cctctccgag ctgctctcac 1081 agctggactc tggagtttcc caggctgtcg agggccccga ggagctcagc cgcagctcct 1141 ctgagtccaa gctgccatcg tccggcagtg ggaagaggct ctcgggggtg tcctcggtgg 1201 actccgcctt ctcttccaga ggatcactgt cgctgtcctt tgagcgggaa ccttcaacca 1261 gcgatctggg caccacagac gtccagaaga agaagcttgt ggatgccatc gtgtccgggg 1321 acaccagcaa actgatgaag atcctgcagc cgcaggacgt ggacctggca ctggacagcg 1381 gtgccagcct gctgcacctg gcggtggagg ccgggcaaga ggagtgcgcc aagtggctgc 1441 tgctcaacaa tgccaacccc aacctgagca accgtagggg ctccaccccg ttgcacatgg 1501 ccgtggagag gagggtgcgg ggtgtcgtgg agctcctgct ggcgcggaag atcagtgtca 1561 acgccaagga tgaggaccag tggacagccc tccactttgc agcccagaac ggggacgagt 1621 ctagcacacg gctgctgttg gagaagaacg cctcggtcaa cgaggtggac tttgagggcc 1681 ggacgcccat gcacgtggcc tgccagcacg ggcaggagaa tatcgtgcgc atcctgctgc 1741 gccgaggcgt ggacgtgagc ctgcagggca aggatgcctg gctgccactg cactacgctg 1801 cctggcaggg ccacctgccc atcgtcaagc tgctggccaa gcagccgggg gtgagtgtga 1861 acgcccagac gctggatggg aggacgccat tgcacctggc cgcacagcgc gggcactacc 1921 gcgtggcccg catcctcatc gacctgtgct ccgacgtcaa cgtctgcagc ctgctggcac 1981 agacacccct gcacgtggcc gcggagacgg ggcacacgag cactgccagg ctgctcctgc 2041 atcggggcgc tggcaaggag gccatgaccT CAGACGGCTA CACCGCTCTG CACCTGGCTG 2101 CCCGCAACGG ACACCTGGCC ACTGTCAAGC TGCTTGTCGA GGAGAAGGCC GATGTGCTGG 2161 CCCGGGGACC CCTGAACCAG ACGGCGCTGC ACCTGGCTGC CGCCCACGGG CACTCGGAGG 2221 TGGTGGAGGA GTTGGTCAGC GCCGATGTCA TTGACCTGTT CGACGAGCAG GGGCTCAGCG 2281 CGCTGCACCT GGCCGCCCAG GGCCGGCACG CACAGACGGT GGAGACTCTG CTCAGGCATG 2341 GGGCCCACAT CAACCTGCAG AGCCTCAAGT TCCAGGGCGG CCATGGCCCC GCCGCCACGC 2401 TCCTGCGGCG AAGCAAGACC TAGAACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2461 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2521 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT CGCCCATACC 2581 GGCACTAACC CATACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2641 att