Construct: ORF TRCN0000489347
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021958.1_s317c1
- DNA Barcode:
- ATCGAATCGTCGATAAACGTCTCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- FCER1G (2207)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489347
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2207 | FCER1G | Fc fragment of IgE receptor Ig | NM_004106.2 | 100% | 100% | |
| 2 | mouse | 14127 | Fcer1g | Fc receptor, IgE, high affi... | NM_010185.4 | 88.3% | 88.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 351
- ORF length:
- 258
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGCAGGCA CCATGATTCC AGCAGTGGTC TTGCTCTTAC 121 TCCTTTTGGT TGAACAAGCA GCGGCCCTGG GAGAGCCTCA GCTCTGCTAT ATCCTGGATG 181 CCATCCTGTT TCTGTATGGA ATTGTCCTCA CCCTCCTCTA CTGTCGACTG AAGATCCAAG 241 TGCGAAAGGC AGCTATAACC AGCTATGAGA AATCAGATGG TGTTTACACG GGCCTGAGCA 301 CCAGGAACCA GGAGACTTAC GAGACTCTGA AGCATGAGAA ACCACCACAG TAGAACCCAG 361 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 421 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 481 TATCTTGTGG AAAGGACGAA TCGAATCGTC GATAAACGTC TCTACGCGTT AAGTCgacaa 541 tcaacctctg gattacaaaa tttgtgaaag att