Construct: ORF TRCN0000489352
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021670.1_s317c1
- DNA Barcode:
- AACCCAGGAACTACATCGAGTCGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CXCL13 (10563)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489352
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10563 | CXCL13 | C-X-C motif chemokine ligan... | NM_001371558.1 | 100% | 100% | |
| 2 | human | 10563 | CXCL13 | C-X-C motif chemokine ligan... | NM_006419.2 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 92
- ORF end:
- 419
- ORF length:
- 327
- Sequence:
-
1 CAGACAGTGG TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGCTA GCATCGATTG 61 ATCAACAAGT TTGTACAAAA AAGCAGGCAC CATGAAGTTC ATCTCGACAT CTCTGCTTCT 121 CATGCTGCTG GTCAGCAGCC TCTCTCCAGT CCAAGGTGTT CTGGAGGTCT ATTACACAAG 181 CTTGAGGTGT AGATGTGTCC AAGAGAGCTC AGTCTTTATC CCTAGACGCT TCATTGATCG 241 AATTCAAATC TTGCCCCGTG GGAATGGTTG TCCAAGAAAA GAAATCATAG TCTGGAAGAA 301 GAACAAGTCA ATTGTGTGTG TGGACCCTCA AGCTGAATGG ATACAAAGAA TGATGGAAGT 361 ATTGAGAAAA AGAAGTTCTT CAACTCTACC AGTTCCAGTG TTTAAGAGAA AGATTCCCTA 421 GAACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT 481 CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG 541 GCTTTATATA TCTTGTGGAA AGGACGAAAC CCAGGAACTA CATCGAGTCG CACGCGTTAA 601 GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t