Construct: ORF TRCN0000489354
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021473.1_s317c1
- DNA Barcode:
- GGCCCGAACCACCTAATGTGCATA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- UMPS (7372)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489354
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7372 | UMPS | uridine monophosphate synth... | NM_000373.4 | 99.6% | 100% | 1440_1441insTGAAC |
| 2 | human | 7372 | UMPS | uridine monophosphate synth... | XR_001740253.2 | 21.1% | (many diffs) | |
| 3 | human | 7372 | UMPS | uridine monophosphate synth... | NR_033437.1 | 21.1% | (many diffs) | |
| 4 | human | 7372 | UMPS | uridine monophosphate synth... | NR_033434.1 | 19.1% | (many diffs) | |
| 5 | mouse | 22247 | Umps | uridine monophosphate synth... | NM_009471.3 | 83.3% | 88.5% | (many diffs) |
| 6 | mouse | 22247 | Umps | uridine monophosphate synth... | NM_001348087.1 | 52.3% | 55% | (many diffs) |
| 7 | mouse | 22247 | Umps | uridine monophosphate synth... | XM_006522008.3 | 52.3% | 55% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1506
- ORF length:
- 1440
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagaat 61 tcaccatggc ggtcgctcgt gcagctttgg ggccattggt gacgggtctg tacgacgtgc 121 aggctttcaa gtttggggac ttcgtgctga agagcgggct ttcctccccc atctacatcg 181 atctgcgggg catcgtgtct cgaccgcgtc ttctgagtca ggttgcagat attttattcc 241 aaactgccca aaatgcaggc atcagttttg acaccgtgtg tggagtgcct tatacagctt 301 tgccattggc tacagttatc tgttcaacca atcaaattcc aatgcttatt agaaggaaag 361 aaacaaagga ttatggaact aagcgtcttg tagaaggaac tattaatcca ggagaaacct 421 gtttaatcat tgaagatgtt gtcaccagtg gatctagtgt tttggaaact gttgaggttc 481 ttcagaagga gggcttgaag gtcactgatg ccatagtgct gttggacaga gagcagggag 541 gcaaggacaa gttgcaggcg cacgggatcc gcctccactc agtgtgtaca ttgtccaaaa 601 tgctggagat tctcgagcag cagaaaaaag ttgatgctga gacagttggg agagtgaaga 661 ggtttattca ggagaatgtc tttgtggcag cgaatcataa tggttctccc ctttctataa 721 aggaagcacc caaagaactc agcttcggtg cacgtgcaga gctgcccagg atccacccag 781 ttgcatcgaa gcttctcagg cttatgcaaa agaaggagac caatctgtgt ctatctgctg 841 atgtttcact ggccagagag ctgttgcagc tagcagatgc tttaggacct agtatctgca 901 tgctgaagac tcatgtagat attttgaatg attttactct ggatgtgatg aaggagttga 961 taactctggc aaaatgccat gagttcttga tatttgaaga ccggaagttt gcagatatag 1021 gaaacacagt gaaaaagcag tatgaaggag gtatctttaa aatagcttcc tgggcagatc 1081 tagtaaatgc tcacgtggtg ccaggctcag gagttgtgaa aggccTGCAA GAAGTGGGCC 1141 TGCCTTTGCA TCGGGGGTGC CTCCTTATTG CGGAAATGAG CTCCACCGGC TCCCTGGCCA 1201 CTGGGGACTA CACTAGAGCA GCGGTTAGAA TGGCTGAGGA GCACTCTGAA TTTGTTGTTG 1261 GTTTTATTTC TGGCTCCCGA GTAAGCATGA AACCAGAATT TCTTCACTTG ACTCCAGGAG 1321 TTCAGTTGGA AGCAGGAGGA GATAATCTTG GCCAACAGTA CAATAGCCCA CAAGAAGTTA 1381 TTGGCAAACG AGGTTCCGAT ATCATCATTG TAGGTCGTGG CATAATCTCA GCAGCTGATC 1441 GTCTGGAAGC AGCAGAGATG TACAGAAAAG CTGCTTGGGA AGCGTATTTG AGTAGACTTG 1501 GTGTTTGAAC TAGTGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1561 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1621 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GGCCCGAACC ACCTAATGTG 1681 CATAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt