Construct: ORF TRCN0000489355
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021429.1_s317c1
- DNA Barcode:
- AGTTTTTAATTCTATTACGGATGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- FRMPD2 (143162)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489355
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 143162 | FRMPD2 | FERM and PDZ domain contain... | XM_011539327.2 | 90.3% | 88.6% | (many diffs) |
| 2 | human | 143162 | FRMPD2 | FERM and PDZ domain contain... | NM_001018071.4 | 46.7% | 45.8% | (many diffs) |
| 3 | human | 143162 | FRMPD2 | FERM and PDZ domain contain... | NM_001318191.1 | 44.6% | 43.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 87
- ORF end:
- 1929
- ORF length:
- 1842
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagcgg 61 ccgccccctt caccggatcc ttttggatgc agcctttaac gaaggacgca ggcatgagcc 121 tgtcctctgt gacgctggcc agcgccctac aggtcagggg tgaagctctg tctgaggagg 181 aaatctggtc cctcctgttc ctggccgctg agcagctcct ggaagacctc cgcaacgatt 241 cctcggacta tgtggtttgc ccctggtcag ccctgctttc tgcagctgga agcctttctt 301 tccaaggccg tgtttctcat atagaggctg ctcctttcaa ggcccctgaa ctgctacagg 361 gacagagtga ggatgagcag cctgatgcat ctcagatgca tgtctattct ttaggaatga 421 ccctctactg gtcagcaggg tttcatgttc cgccacatca gcccctgcag ctctgcgagc 481 ccctgcactc catcctgctg accatgtgtg aagaccagcc tcacaggcgg tgcacgttgc 541 agtcggttct ggaagcttgt cgggttcatg agaaagaagt gtctgtctac ccagcccctg 601 ctggtctcca catcagaagg ctggttggct tggttctggg taccatttct gaggtggaga 661 aaagagttgt ggaggaaagc tcctctgtgc agcagaacag aagctacctg ctcaggaaga 721 ggctgcgtgg gacaagcagc gagagcccag cggcacaggc cccggagtgt ctgcatcctt 781 gcagagtttc agaaagaagc acggagaccc agagctcacc agagccccat tggagcacct 841 tgacacacag tcactgcagc ctccttgtta accgcgctct tccaggagca gatccccagg 901 accagcaggc gggccggagg ctcagctctg gatctgtgca ctcggcagca gacagctcat 961 ggccaacaac tccttctcag aggggttttc tgcaaagaag gagcaagttt tccaggccag 1021 agttcatcct gttggctgga gaggccccga tgacactaca tctgccggga tcggttgtga 1081 ccaaaaaagg gaaatcctat ttggctctca gggacctctg tgtggtcctg ctgaacgggc 1141 agcacctgga ggtaaaatgt gatgttgaat caacagtggg agctgtcttc aatgccgtga 1201 catcctttgc caacctcgag gaactcacct actttggctt ggcgtatatg aaaagcaaag 1261 agttcttttt cctggacagt gaaaccagat tgtgcaaaat agctcctgaa ggctggagag 1321 agcagcctca gaagacctcc atgaatacct tcacactctt cctgaggata aagttctttg 1381 tcagccacta tgggctgctc cagcacagcc tgacaaggca ccagttttac ctgcagcttc 1441 ggaaagatat cctggaggag aggctgtact gcaatgaaga gatactgctg cagctggggg 1501 tccttgcctT GCAGGCTGAG TTTGGCAATT ACCCTAAGGA GCAGGTGGAG AGTAAGCCAT 1561 ACTTTCACGT TGAAGATTAC ATCCCAGCGA GTCTGATCGA GAGGATGACC GCTCTACGGG 1621 TCCAGGTTGA AGTCTCAGAG ATGCACCGGC TCAGCTCTGC ACTGTGGGGA GAGGATGCTG 1681 AGCTGAAGTT CTTGAGGGTC ACTCAGCAGC TCCCAGAATA CGGTGTGCTG GTTCACCAAG 1741 TATTCTCAGA GAAGAGGAGG CCAGAAGAGG AGATGGCCCT GGGGATCTGT GCCAAGGGTG 1801 TCATAGTCTA TGAAGTGAAA AACAACAGCA GAATTGCAAT GTTACGGTTT CAGTGGAGAG 1861 AAACCGGGAA GATTTCTACT TATGTGAGTG TAGCTCAGTT GATAAATAAC CTGCACTTCA 1921 TTTTCCTTTG AAGGGTGGGC GCGCCGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG 1981 TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA 2041 ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AAGTTTTTAA 2101 TTCTATTACG GATGTACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 2161 agatt