Transcript: Human NM_001318191.1

Homo sapiens FERM and PDZ domain containing 2 (FRMPD2), transcript variant 5, mRNA.

Source:
NCBI, updated 2018-07-01
Taxon:
Homo sapiens (human)
Gene:
FRMPD2 (143162)
Length:
4665
CDS:
48..3902

Additional Resources:

NCBI RefSeq record:
NM_001318191.1
NBCI Gene record:
FRMPD2 (143162)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001318191.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000358754 ATTCAGCCAAGACCAGTAAAT pLKO_005 1828 CDS 100% 13.200 10.560 N FRMPD2 n/a
2 TRCN0000378325 CTACTTTGGCTTGGCGTATAT pLKO_005 1118 CDS 100% 13.200 9.240 N FRMPD2 n/a
3 TRCN0000082785 CCATGATAAGTTTGTGCAAAT pLKO.1 1928 CDS 100% 10.800 7.560 N FRMPD2 n/a
4 TRCN0000082784 GCTGAGTACATCAATGGATAA pLKO.1 2066 CDS 100% 10.800 7.560 N FRMPD2 n/a
5 TRCN0000082787 CCTCAGAAGACCTCCATGAAT pLKO.1 1215 CDS 100% 5.625 3.938 N FRMPD2 n/a
6 TRCN0000256106 AGCTTGCCGCATCTCTAAATA pLKO_005 4112 3UTR 100% 15.000 7.500 Y FRMPD2B n/a
7 TRCN0000256108 CCAGACTTTCGACAGATATTT pLKO_005 3877 CDS 100% 15.000 7.500 Y FRMPD2B n/a
8 TRCN0000256339 GGTTCCAAAGAAGTCAATAAA pLKO_005 4420 3UTR 100% 15.000 7.500 Y FRMPD2B n/a
9 TRCN0000256340 ACCAGACTTTCGACAGATATT pLKO_005 3876 CDS 100% 13.200 6.600 Y FRMPD2B n/a
10 TRCN0000256109 AGTGATCTTGTGAGGATTAAG pLKO_005 3288 CDS 100% 13.200 6.600 Y FRMPD2B n/a
11 TRCN0000265795 CATGAGACTTGGAAGATATTC pLKO_005 3839 CDS 100% 13.200 6.600 Y FRMPD2B n/a
12 TRCN0000265796 CCATTGCAGCTGGTGACATTA pLKO_005 3346 CDS 100% 13.200 6.600 Y FRMPD2B n/a
13 TRCN0000256338 TCCTGAGTCCCACCCTCATTT pLKO_005 3728 CDS 100% 13.200 6.600 Y FRMPD2B n/a
14 TRCN0000256105 ACATTATCCTGGCCGTGAATG pLKO_005 3361 CDS 100% 10.800 5.400 Y FRMPD2B n/a
15 TRCN0000082783 CGCATCTCTAAATAGGCAAAT pLKO.1 4119 3UTR 100% 10.800 5.400 Y FRMPD2 n/a
16 TRCN0000256107 GAAAGGGATGAATCAACATTG pLKO_005 3765 CDS 100% 10.800 5.400 Y FRMPD2B n/a
17 TRCN0000082786 GTGAGGATTAAGAGGCTCTTT pLKO.1 3297 CDS 100% 4.950 2.475 Y FRMPD2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001318191.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489355 AGTTTTTAATTCTATTACGGATGT pLX_317 23.5% 44.6% 43.5% V5 (not translated due to prior stop codon) (many diffs) n/a
2 ccsbBroadEn_13214 pDONR223 100% 20.3% 20.3% None 1_3069del n/a
3 ccsbBroad304_13214 pLX_304 0% 20.3% 20.3% V5 1_3069del n/a
4 TRCN0000467450 ACAATAACCCCACTAAAGACAATA pLX_317 52.6% 20.3% 20.3% V5 1_3069del n/a
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