Construct: ORF TRCN0000489359
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020925.1_s317c1
- DNA Barcode:
- AATGTGCTTGCAATTCCCTGCTGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HRH1 (3269)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489359
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3269 | HRH1 | histamine receptor H1 | NM_000861.3 | 99.9% | 99.7% | 1369G>A |
| 2 | human | 3269 | HRH1 | histamine receptor H1 | NM_001098211.2 | 99.9% | 99.7% | 1369G>A |
| 3 | human | 3269 | HRH1 | histamine receptor H1 | NM_001098212.2 | 99.9% | 99.7% | 1369G>A |
| 4 | human | 3269 | HRH1 | histamine receptor H1 | NM_001098213.2 | 99.9% | 99.7% | 1369G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1533
- ORF length:
- 1461
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagcctc cccaattcct cctgcctctt agaagacaag atgtgtgagg 121 gcaacaagac cactatggcc agcccccagc tgatgcccct ggtggtggtc ctgagcacta 181 tctgcttggt cacagtaggg ctcaacctgc tggtgctgta tgccgtacgg agtgagcgga 241 agctccacac tgtggggaac ctgtacatcg tcagcctctc ggtggcggac ttgatcgtgg 301 gtgccgtcgt catgcctatg aacatcctct acctgctcat gtccaagtgg tcactgggcc 361 gtcctctctg cctcttttgg ctttccatgg actatgtggc cagcacagcg tccattttca 421 gtgtcttcat cctgtgcatt gatcgctacc gctctgtcca gcagcccctc aggtacctta 481 agtatcgtac caagacccga gcctcggcca ccattctggg ggcctggttt ctctcttttc 541 tgtgggttat tcccattcta ggctggaatc acttcatgca gcagacctcg gtgcgccgag 601 aggacaagtg tgagacagac ttctatgatg tcacctggtt caaggtcatg actgccatca 661 tcaacttcta cctgcccacc ttgctcatgc tctggttcta tgccaagatc tacaaggccg 721 tacgacaaca ctgccagcac cgggagctca tcaataggtc cctcccttcc ttctcagaaa 781 ttaagctgag gccagagaac cccaaggggg atgccaagaa accagggaag gagtctccct 841 gggaggttct gaaaaggaag ccaaaagatg ctggtggtgg atctgtcttg aagtcaccat 901 cccaaacccc caaggagatg aaatccccag ttgtcttcag ccaagaggat gatagagaag 961 tagacaaact ctactgcttt ccacttgata ttgtgcacat gcaggctgcg gcagagggga 1021 gtagcaggga ctatgtagcc gtcaaccgga gccatggcca gctcaagaca gatgagcagg 1081 gcctgaacac acatggggcc agcgagatat cagaggatca gatgttaggt gatagccaat 1141 ccttctctcg aacggacTCA GATACCACCA CAGAGACAGC ACCAGGCAAA GGCAAATTGA 1201 GGAGTGGGTC TAACACAGGC CTGGATTACA TCAAGTTTAC TTGGAAGAGG CTCCGCTCGC 1261 ATTCAAGACA GTATGTATCT GGGTTGCACA TGAACCGCGA AAGGAAGGCC GCCAAACAGT 1321 TGGGTTTTAT CATGGCAGCC TTCATCCTCT GCTGGATCCC TTATTTCATC TTCTTCATGG 1381 TCATTGCCTT CTGCAAGAAC TGTTGCAATG AACATTTGCA CATGTTCACC ATCTGGCTGA 1441 GCTACATCAA CTCCACACTG AACCCCCTCA TCTACCCCTT GTGCAATGAG AACTTCAAGA 1501 AGACATTCAA GAGAATTCTG CATATTCGCT CCTAGGACCC AGCTTTCTTG TACAAAGTGG 1561 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1621 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1681 AAATGTGCTT GCAATTCCCT GCTGTACGCG TTAAGTCgac aatcaacctc tggattacaa 1741 aatttgtgaa agatt