Construct: ORF TRCN0000489376
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019609.1_s317c1
- DNA Barcode:
- AGAGGCTGACGGACTCCCGCAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HRH1 (3269)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489376
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3269 | HRH1 | histamine receptor H1 | NM_000861.3 | 99.8% | 99.5% | 1369G>A;1461_1462insG |
| 2 | human | 3269 | HRH1 | histamine receptor H1 | NM_001098211.2 | 99.8% | 99.5% | 1369G>A;1461_1462insG |
| 3 | human | 3269 | HRH1 | histamine receptor H1 | NM_001098212.2 | 99.8% | 99.5% | 1369G>A;1461_1462insG |
| 4 | human | 3269 | HRH1 | histamine receptor H1 | NM_001098213.2 | 99.8% | 99.5% | 1369G>A;1461_1462insG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1539
- ORF length:
- 1464
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgagc ctccccaatt cctcctgcct cttagaagac aagatgtgtg 121 agggcaacaa gaccactatg gccagccccc agctgatgcc cctggtggtg gtcctgagca 181 ctatctgctt ggtcacagta gggctcaacc tgctggtgct gtatgccgta cggagtgagc 241 ggaagctcca cactgtgggg aacctgtaca tcgtcagcct ctcggtggcg gacttgatcg 301 tgggtgccgt cgtcatgcct atgaacatcc tctacctgct catgtccaag tggtcactgg 361 gccgtcctct ctgcctcttt tggctttcca tggactatgt ggccagcaca gcgtccattt 421 tcagtgtctt catcctgtgc attgatcgct accgctctgt ccagcagccc ctcaggtacc 481 ttaagtatcg taccaagacc cgagcctcgg ccaccattct gggggcctgg tttctctctt 541 ttctgtgggt tattcccatt ctaggctgga atcacttcat gcagcagacc tcggtgcgcc 601 gagaggacaa gtgtgagaca gacttctatg atgtcacctg gttcaaggtc atgactgcca 661 tcatcaactt ctacctgccc accttgctca tgctctggtt ctatgccaag atctacaagg 721 ccgtacgaca acactgccag caccgggagc tcatcaatag gtccctccct tccttctcag 781 aaattaagct gaggccagag aaccccaagg gggatgccaa gaaaccaggg aaggagtctc 841 cctgggaggt tctgaaaagg aagccaaaag atgctggtgg tggatctgtc ttgaagtcac 901 catcccaaac ccccaaggag atgaaatccc cagttgtctt cagccaagag gatgatagag 961 aagtagacaa actctactgc tttccacttg atattgtgca catgcaggct gcggcagagg 1021 ggagtagcag ggactatgta gccgtcaacc ggagccatgg ccagctcaag acagatgagc 1081 agggcctgaa cacacatggg gccagcgaga tatcagagga tcagatgtta ggtgatagcc 1141 aaTCCTTCTC TCGAACGGAC TCAGATACCA CCACAGAGAC AGCACCAGGC AAAGGCAAAT 1201 TGAGGAGTGG GTCTAACACA GGCCTGGATT ACATCAAGTT TACTTGGAAG AGGCTCCGCT 1261 CGCATTCAAG ACAGTATGTA TCTGGGTTGC ACATGAACCG CGAAAGGAAG GCCGCCAAAC 1321 AGTTGGGTTT TATCATGGCA GCCTTCATCC TCTGCTGGAT CCCTTATTTC ATCTTCTTCA 1381 TGGTCATTGC CTTCTGCAAG AACTGTTGCA ATGAACATTT GCACATGTTC ACCATCTGGC 1441 TGAGCTACAT CAACTCCACA CTGAACCCCC TCATCTACCC CTTGTGCAAT GAGAACTTCA 1501 AGAAGACATT CAAGAGAATT CTGCATATTC GCTCCGACCC AGCTTTCTTG TACAAAGTGG 1561 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1621 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1681 AAGAGGCTGA CGGACTCCCG CAACTACGCG TTAAGTCgac aatcaacctc tggattacaa 1741 aatttgtgaa agatt