Construct: ORF TRCN0000489383
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021439.1_s317c1
- DNA Barcode:
- AGAAAGGTTCTACATGCGCCGCGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PTEN (5728)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489383
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5728 | PTEN | phosphatase and tensin homolog | NM_000314.8 | 100% | 100% | |
| 2 | human | 5728 | PTEN | phosphatase and tensin homolog | NM_001304717.5 | 69.9% | 69.9% | 1_519del |
| 3 | human | 5728 | PTEN | phosphatase and tensin homolog | NM_001304718.2 | 51.1% | 51.1% | 0_1ins591 |
| 4 | human | 11191 | PTENP1 | phosphatase and tensin homo... | NR_023917.1 | 30.2% | (many diffs) | |
| 5 | mouse | 19211 | Pten | phosphatase and tensin homolog | NM_008960.2 | 96.1% | 99.7% | (many diffs) |
| 6 | mouse | 19211 | Pten | phosphatase and tensin homolog | XM_006526769.2 | 63.8% | 66.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1278
- ORF length:
- 1209
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gacagccatc atcaaagaga tcgttagcag aaacaaaagg agatatcaag 121 aggatggatt cgacttagac ttgacctata tttatccaaa cattattgct atgggatttc 181 ctgcagaaag acttgaaggc gtatacagga acaatattga tgatgtagta aggtttttgg 241 attcaaagca taaaaaccat tacaagatat acaatctttg tgctgaaaga cattatgaca 301 ccgccaaatt taattgcaga gttgcacaat atccttttga agaccataac ccaccacagc 361 tagaacttat caaacccttt tgtgaagatc ttgaccaatg gctaagtgaa gatgacaatc 421 atgttgcagc aattcactgt aaagctggaa agggacgaac tggtgtaatg atatgtgcat 481 atttattaca tcggggcaaa tttttaaagg cacaagaggc cctagatttc tatggggaag 541 taaggaccag agacaaaaag ggagtaacta ttcccagtca gaggcgctat gtgtattatt 601 atagctacct gttaaagaat catctggatt atagaccagt ggcactgttg tttcacaaga 661 tgatgtttga aactattcca atgttcagtg gcggaacttg caatcctcag tttgtggtct 721 gccagctaaa ggtgaagata tattcctcca attcaggacc cacacgacgg gaagacaagt 781 tcatgtactt tgagttccct cagccgttac ctgtgtgtgg tgatatcaaa gtagagttct 841 tccacaaaca gaacaagatg ctaaaaaagg acaaaatgtt tcacttttGG GTAAATACAT 901 TCTTCATACC AGGACCAGAG GAAACCTCAG AAAAAGTAGA AAATGGAAGT CTATGTGATC 961 AAGAAATCGA TAGCATTTGC AGTATAGAGC GTGCAGATAA TGACAAGGAA TATCTAGTAC 1021 TTACTTTAAC AAAAAATGAT CTTGACAAAG CAAATAAAGA CAAAGCCAAC CGATACTTTT 1081 CTCCAAATTT TAAGGTGAAG CTGTACTTCA CAAAAACAGT AGAGGAGCCG TCAAATCCAG 1141 AGGCTAGCAG TTCAACTTCT GTAACACCAG ATGTTAGTGA CAATGAACCT GATCATTATA 1201 GATATTCTGA CACCACTGAC TCTGATCCAG AGAATGAACC TTTTGATGAA GATCAGCATA 1261 CACAAATTAC AAAAGTCTGA GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1321 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1381 AAAGATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGAAA GGTTCTACAT 1441 GCGCCGCGGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt