Construct: ORF TRCN0000489384
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021430.1_s317c1
- DNA Barcode:
- TGCGGGGTTCGTTTACACCGACCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DUSP26 (78986)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489384
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 78986 | DUSP26 | dual specificity phosphatas... | NM_001305115.1 | 100% | 100% | |
| 2 | human | 78986 | DUSP26 | dual specificity phosphatas... | NM_001305116.1 | 100% | 100% | |
| 3 | human | 78986 | DUSP26 | dual specificity phosphatas... | NM_024025.3 | 100% | 100% | |
| 4 | mouse | 66959 | Dusp26 | dual specificity phosphatas... | NM_025869.3 | 90.6% | 96.6% | (many diffs) |
| 5 | mouse | 66959 | Dusp26 | dual specificity phosphatas... | XM_017312942.1 | 90.6% | 96.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 723
- ORF length:
- 633
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagcgg 61 ccgccccctt caccggatcc acctctaaga tgtgccctgg taactggctt tgggcttcta 121 tgacttttat ggcccgcttc tcccggagta gctcaaggtc tcctgttcga actcgaggga 181 ccctggagga gatgccaacc gttcaacatc ctttcctcaa tgtcttcgag ttggagcggc 241 tcctctacac aggcaagaca gcctgtaacc atgccgacga ggtctggcca ggcctctatc 301 tcggagacca ggacatggct aacaaccgcc gggagcttcg ccgccTGGGC ATCACGCACG 361 TCCTCAATGC CTCACACAGC CGGTGGCGAG GCACGCCCGA GGCCTATGAG GGGCTGGGCA 421 TCCGCTACCT GGGTGTTGAG GCCCACGACT CGCCAGCCTT TGACATGAGC ATCCACTTCC 481 AGACGGCTGC CGACTTCATC CACCGGGCGC TGAGCCAGCC AGGAGGGAAG ATCCTGGTGC 541 ATTGTGCTGT GGGCGTGAGC CGATCCGCCA CCCTGGTACT GGCCTACCTC ATGCTGTACC 601 ACCACCTTAC CCTCGTGGAG GCCATCAAGA AAGTCAAAGA CCACCGAGGC ATCATCCCCA 661 ACCGGGGCTT CCTGAGGCAG CTCCTGGCCC TGGACCGCAG GCTGCGGCAG GGTCTGGAAG 721 CATGAAAGGG TGGGCGCGCC GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 781 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 841 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATGCG GGGTTCGTTT 901 ACACCGACCA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt