Construct: ORF TRCN0000489385
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021312.1_s317c1
- DNA Barcode:
- TAGAGGTGGTATGTAAGCGCAGTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- OTUB2 (78990)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489385
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 78990 | OTUB2 | OTU deubiquitinase, ubiquit... | NM_023112.4 | 31.1% | 31.1% | 220_702del |
| 2 | mouse | 68149 | Otub2 | OTU domain, ubiquitin aldeh... | NM_026580.4 | 29% | 29.4% | (many diffs) |
| 3 | mouse | 68149 | Otub2 | OTU domain, ubiquitin aldeh... | XM_006516185.3 | 24.4% | 24.5% | (many diffs) |
| 4 | mouse | 68149 | Otub2 | OTU domain, ubiquitin aldeh... | NM_001177841.1 | 21% | 21% | (many diffs) |
| 5 | mouse | 68149 | Otub2 | OTU domain, ubiquitin aldeh... | NR_033587.1 | 6.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 312
- ORF length:
- 219
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGCAGGCA CCATGAGTGA AACATCTTTC AACCTAATAT 121 CAGAAAAATG TGACATTCTA TCCATTCTTC GGGACCATCC TGAAAACAGG ATTTACCGGA 181 GGAAAATCGA GGAACTCAGC AAAAGGTTCA CCGCCATCCG CAAGACCAAA GGGGATGGGA 241 ACTGCTTCTA CAGGGCCTTG GGCTATTCCT ACCTGGAGTC CCTGCTGGGG AAGAGCAGGG 301 AGATCTTCAA GTAGAACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 361 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 421 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TAGAGGTGGT ATGTAAGCGC 481 AGTGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt