Construct: ORF TRCN0000489392
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020788.1_s317c1
- DNA Barcode:
- ACAAACATAGGACCCCGTAGGATA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CYSLTR2 (57105)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489392
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_001308465.3 | 100% | 100% | |
| 2 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_001308467.3 | 100% | 100% | |
| 3 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_001308468.3 | 100% | 100% | |
| 4 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_001308469.3 | 100% | 100% | |
| 5 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_001308470.3 | 100% | 100% | |
| 6 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_001308471.3 | 100% | 100% | |
| 7 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_001308476.3 | 100% | 100% | |
| 8 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_020377.5 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1110
- ORF length:
- 1038
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagaga aaatttatgt ccttgcaacc atccatctcc gtatcagaaa 121 tggaaccaaa tggcaccttc agcaataaca acagcaggaa ctgcacaatt gaaaacttca 181 agagagaatt tttcccaatt gtatatctga taatattttt ctggggagtc ttgggaaatg 241 ggttgtccat atatgttttc ctgcagcctt ataagaagtc cacatctgtg aacgttttca 301 tgctaaatct ggccatttca gatctcctgt tcataagcac gcttcccttc agggctgact 361 attatcttag aggctccaat tggatatttg gagacctggc ctgcaggatt atgtcttatt 421 ccttgtatgt caacatgtac agcagtattt atttcctgac cgtgctgagt gttgtgcgtt 481 tcctggcaat ggttcacccc tttcggcttc tgcatgtcac cagcatcagg agtgcctgga 541 tcctctgtgg gatcatatgg atccttatca tggcttcctc aataatgctc ctggacagtg 601 gctctgagca gaacggcagt gtcacatcat gcttagagct gaatctctat aaaattgcTA 661 AGCTGCAGAC CATGAACTAT ATTGCCTTGG TGGTGGGCTG CCTGCTGCCA TTTTTCACAC 721 TCAGCATCTG TTATCTGCTG ATCATTCGGG TTCTGTTAAA AGTGGAGGTC CCAGAATCGG 781 GGCTGCGGGT TTCTCACAGG AAGGCACTGA CCACCATCAT CATCACCTTG ATCATCTTCT 841 TCTTGTGTTT CCTGCCCTAT CACACACTGA GGACCGTCCA CTTGACGACA TGGAAAGTGG 901 GTTTATGCAA AGACAGACTG CATAAAGCTT TGGTTATCAC ACTGGCCTTG GCAGCAGCCA 961 ATGCCTGCTT CAATCCTCTG CTCTATTACT TTGCTGGGGA GAATTTTAAG GACAGACTAA 1021 AGTCTGCACT CAGAAAAGGC CATCCACAGA AGGCAAAGAC AAAGTGTGTT TTCCCTGTTA 1081 GTGTGTGGTT GAGAAAGGAA ACAAGAGTAT AGTACTCAGC TTACTTGTAC AAAGTGGTTG 1141 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1201 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAC 1261 AAACATAGGA CCCCGTAGGA TAACGCGTTA AGTCgacaat caacctctgg attacaaaat 1321 ttgtgaaaga tt