Construct: ORF TRCN0000489405
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019206.1_s317c1
- DNA Barcode:
- CCACGGGAACATATGGTACGAGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADGRF5 (221395)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489405
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | NM_001098518.2 | 99.8% | 99.8% | (many diffs) |
| 2 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | NM_015234.5 | 99.8% | 99.8% | (many diffs) |
| 3 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_011514353.3 | 98.4% | 98.1% | (many diffs) |
| 4 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_017010419.2 | 98.4% | 98.1% | (many diffs) |
| 5 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_017010420.2 | 98.4% | 98.1% | (many diffs) |
| 6 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_017010421.2 | 98.4% | 98.1% | (many diffs) |
| 7 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_005248892.3 | 96.9% | 96.9% | (many diffs) |
| 8 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_005248893.3 | 96.9% | 96.9% | (many diffs) |
| 9 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_005248894.4 | 95.6% | 95.3% | (many diffs) |
| 10 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_005248895.4 | 95.6% | 95.3% | (many diffs) |
| 11 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_017010418.2 | 95.6% | 95.3% | (many diffs) |
| 12 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_017010422.2 | 92% | 92% | (many diffs) |
| 13 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_017010423.1 | 86.9% | 86.9% | (many diffs) |
| 14 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_017010424.2 | 85.6% | 85.2% | (many diffs) |
| 15 | human | 221395 | ADGRF5 | adhesion G protein-coupled ... | XM_011514354.2 | 84.4% | 84.4% | (many diffs) |
| 16 | human | 112268033 | LOC112268033 | uncharacterized LOC112268033 | XR_002956835.1 | 2.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 4113
- ORF length:
- 4038
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgaaa tccccaagga gaaccacttt gtgcctcatg tttattgtga 121 tttattcttc caaagctgca ctgaactgga attacgagtc tactattcat cctttgagtc 181 ttcatgaaca tgaaccagct ggtgaagagg cactgaggca aaaacgagcc gttgccacaa 241 aaagtcctac ggctgaagaa tacactgtta atattgagat cagttttgaa aatgcatcct 301 tcctggatcc tatcaaagcc tacttgaaca gcctcagttt tccaattcat gggaataaca 361 ctgaccaaat taccgacatt ttgagcataa atgtgacaac agtctgcaga cctgctggaa 421 atgaaatctg gtgctcctgc gagacaggtt atgggtggcc tcgggaaagg tgtcttcaca 481 atctcatttg tcaagagcgt gacgtcttcc tcccagggca ccattgcagt tgccttaaag 541 aactgcctcc caatggacct ttttgcctgc ttcaggaaga tgttaccctg aacatgagag 601 tcagactaaa tgtaggcttt caagaagacc tcatgaacac ttcctccgcc ctctataggt 661 cctacaagac cgacttggaa acagcgttcc ggaagggtta cggaatttta ccaggcttca 721 agggcgtgac tgtgacaggg ttcaagtctg gaagtgtggt tgtgacatat gaagtcaaga 781 ctacaccacc atcacttgag ttaatacata aagccaatga acaagttgta cagagcctca 841 atcagaccta caaaatggac tacaactcct ttcaagcagt tactatcaat gaaagcaatt 901 tctttgtcac accagaaatc atctttgaag gggacacagt cagtctggtg tgtgaaaagg 961 aagttttgtc ctccaatgtg tcttggcgct atgaagaaca gcagttggaa atccagaaca 1021 gcagcagatt ctcgatttac accgcacttt tcaacaacat gacttcggtg tccaagctca 1081 ccatccacaa catcactcca ggtgatgcag gtgaatatgt ttgcaaactg atattagaca 1141 tttttgaata tgagtgcaag aagaaaatag atgttatgcc catccaaatt ttggcaaatg 1201 aagaaatgaa ggtgatgtgc gacaacaatc ctgtatcttt gaactgctgc agtcagggta 1261 atgttaattg gagcaaagta gaatggaagc aggaaggaaa aataaatatt ccaggaaccc 1321 ctgagacaga catagattct agctgcagca gatacaccct caaggctgat ggaacccagt 1381 gcccaagcgg gtcgtctgga acaacagtca tctacacttg tgagttcatc agtgcctatg 1441 gagccagagg cagtgcaaac ataaaagtga cattcatctc tgtggccaat ctaacaataa 1501 ccccggaccc aatttctgtt tctgagggac aaaacttttc tataaaatgc atcagtgatg 1561 tgagtaacta tgatgaggtt tattggaaca cttctgctgg aattaaaata taccaaagat 1621 tttataccac gaggaggtat cttgatggag cagaatcagt actgacagtc aagacctcga 1681 ccagggagtg gaatggaacc tatcactgca tatttagata taagaattca tacagtattg 1741 caaccaaaga cgtcattgtt cacccgctgc ctctaaagct gaacatcatg gttgatcctt 1801 tggaagctac tgtttcatgc agtggttccc atcacatcaa gtgctgcata gaggaggatg 1861 gagactacaa agttactttc catatgggtt cctcatccct tcctgctgca aaagaagtta 1921 acaaaaaaca agtgtgctac aaacacaatt tcaatgcaag ctcagtttcc tggtgttcaa 1981 aaactgttga tgtgtgttgt cactttacca atgctgctaa taattcagtt tggagcccat 2041 ctatgaagct gaatctggtt cctggggaaa acatcacatg ccaggatccc gtaataggtg 2101 tcggagagcc ggggaaagtc atccagaagc tatgccggtt ctcaaacgtt cccagcagcc 2161 ctgagagtcc cattggcggg accatcactt acaaatgtgt aggctcccag tgggaggaga 2221 agagaaatga ctgcatctct gccccaataa acagtctgct ccagatggct aaggctttga 2281 tcaagagccc ctctcaggat gagatgctcc ctacatacct gaaggatctt tctattagca 2341 tagacaaagc ggaacatgaa atcagctctt ctcctgggag tctgggagcc attattaaca 2401 tccttgatct gctctcaaca gttccaaccc aagtaaattc agaaatgatg acgcacgtgc 2461 tctctacggt taatgtcatc cttggcaagc ccgtcttgaa cacctggaag gttttacaac 2521 agcaatggac caatcagagt tcacagctac tacattcagt ggaaagattt tcccaagcat 2581 tacagtcagg agatagccct cctttgtcct tctcccaaac taatgtgcag atgagcagca 2641 cggtaatcaa gtccagccac ccagaaacct atcaacagag gtttgttttc ccatactttg 2701 acctctgggg caatgtggtc attgacaaga gctatctaga aaacttgcag tcggattcgt 2761 ctattgtcac catggctttc ccaactctcc aagccatcct tgctcaggat atccaggaaa 2821 ataactttgc agagagctta gtgatgacaa ccactgtcag ccacaatacg actatgccat 2881 tcaggatttc aatgactttt aagaacaata gcccttcagg cggcgaaacg aagtgtgtct 2941 tctggaactt caggcttgcc aacaacacag gggggtggga cagcagtggg tgctatgttg 3001 aagaaggtga tggggacaat gtcacctgta tctgtgacca cctaacatca ttctccatcc 3061 tcatgtcccc tgactcccca gatcctagtt ctctcctggg aatactcctg gatattattt 3121 cttatgttgg ggtgggcttt tccatcttga gcttggcagc ctgtctagtt gtggaagctg 3181 tggtgtggaa atcggtgacc aagaatcgga cttcttatat gcgccacacc tgcatagtga 3241 atatcgctgc ctcccttctg gtcgccaaca cctggttcat tgtggtcgct gccatccagg 3301 acaatcgcta catactctgc aagacagcct gtgtggctgc caccttcttc atccacttct 3361 tctacctcag cgtcttcttc tggatgctga cactgggcct catgctgttc tatcgcctgg 3421 ttttcattct gcatgaaaca agcaggtcca ctcagaaagc cattgccttc tgtcttggct 3481 atggctgccc acttgccatc tcggtcatca cgctgggagc cacccagccc cgggaagtct 3541 atacgaggaa gaatgtctgt tggctcaact gggaggacac caaggccctg ctggctttcg 3601 ccatcccagc actgatcatt gtggtggtga acataaccat cactattgtg gtcatcacca 3661 agatcctgag gccttccatt ggagacaagc catgcaagca ggagaagagc agcctgtttc 3721 agatcagcaa gagcattggg gtcctcacac cactcttggg cctcacttgg ggttttggTC 3781 TCACCACTGT GTTCCCAGGG ACCAACCTTG TGTTCCATAT CATATTTGCC ATCCTCAATG 3841 TCTTCCAGGG ATTATTCATT TTACTCTTTG GATGCCTCTG GGATCTGAAG GTACAGGAAG 3901 CTTTGCTGAA TAAGTTTTCA TTGTCGAGAT GGTCTTCACA GCACTCAAAG TCAACATCCC 3961 TGGGTTCATC CACACCTGTG TTTTCTATGA GTTCTCCAAT ATCAAGGAGA TTTAACAATT 4021 TGTTTGGTAA AACAGGAACG TATAATGTTT CCACCCCAGA AGCAACCAGC TCATCCCTGG 4081 AAAACTCATC CAGTGCTTCT TCGTTGCTCA ACGACCCAGC TTTCTTGTAC AAAGTGGTTG 4141 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 4201 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 4261 ACGGGAACAT ATGGTACGAG CAACGCGTTA AGTCgacaat caacctctgg attacaaaat 4321 ttgtgaaaga tt