Construct: ORF TRCN0000489415
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019574.1_s317c1
- DNA Barcode:
- GCGCCCGCCACACGCTCCTAATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LPAR6 (10161)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489415
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10161 | LPAR6 | lysophosphatidic acid recep... | NM_001162497.2 | 99.9% | 99.7% | 1032_1033insG |
| 2 | human | 10161 | LPAR6 | lysophosphatidic acid recep... | NM_001162498.2 | 99.9% | 99.7% | 1032_1033insG |
| 3 | human | 10161 | LPAR6 | lysophosphatidic acid recep... | NM_005767.6 | 99.9% | 99.7% | 1032_1033insG |
| 4 | human | 10161 | LPAR6 | lysophosphatidic acid recep... | XM_024449302.1 | 99.9% | 99.7% | 1032_1033insG |
| 5 | mouse | 67168 | Lpar6 | lysophosphatidic acid recep... | NM_175116.4 | 87.3% | 92.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1110
- ORF length:
- 1035
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggta agcgttaaca gctcccactg cttctataat gactccttta 121 agtacacttt gtatgggtgc atgttcagca tggtgtttgt gcttgggtta atatccaatt 181 gtgttgccat atacattttc atctgcgtcc tcaaagtccg aaatgaaact acaacttaca 241 tgattaactt ggcaatgtca gacttgcttt ttgtttttac tttacccttc aggatttttt 301 acttcacaac acggaattgg ccatttggag atttactttg taagatttct gtgatgctgt 361 tttataccaa catgtacgga agcattctgt tcttaacctg tattagtgta gatcgatttc 421 tggcaattgt ctacccattt aagtcaaaga ctctaagaac caaaagaaat gcaaagattg 481 tttgcactgg cgtgtggtta actgtgatcg gaggaagtgc acccgccgtt tttgttcagt 541 ctacccactc tcagggtaac aatgcctcag aagcctgctt tgaaaatttt ccagaagcca 601 catggaaaac atatctctca aggattgtaa ttttcatcga aatagtggga ttttttattc 661 ctctaatttt aaatgtaact tgttctagta tggtgctaaa aactttaacc aaacctgtta 721 cattaagtag aagcaaaata aacaaaacta aggttttaaa aatgattttt gtacatttga 781 tcatattctg ttTCTGTTTT GTTCCTTACA ATATCAATCT TATTTTATAT TCTCTTGTGA 841 GAACACAAAC ATTTGTTAAT TGCTCAGTAG TGGCAGCAGT AAGGACAATG TACCCAATCA 901 CTCTCTGTAT TGCTGTTTCC AACTGTTGTT TTGACCCTAT AGTTTACTAC TTTACATCGG 961 ACACAATTCA GAATTCAATA AAAATGAAAA ACTGGTCTGT CAGGAGAAGT GACTTCAGAT 1021 TCTCTGAAGT TCATGGTGCA GAGAATTTTA TTCAGCATAA CCTACAGACC TTAAAAAGTA 1081 AGATATTTGA CAATGAATCT GCTGCCGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1141 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1201 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCGCCCGC 1261 CACACGCTCC TAATCGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1321 aagatt