Construct: ORF TRCN0000489423
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021962.1_s317c1
- DNA Barcode:
- AGAGCTGATTCGGCAGCCTACGGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HDAC5 (10014)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489423
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10014 | HDAC5 | histone deacetylase 5 | NM_005474.5 | 99.9% | 99.9% | 1779C>G |
| 2 | human | 10014 | HDAC5 | histone deacetylase 5 | XM_005256906.4 | 99.9% | 99.9% | 1779C>G |
| 3 | human | 10014 | HDAC5 | histone deacetylase 5 | NM_001015053.2 | 99.8% | 99.8% | 22_24delGCA;1782C>G |
| 4 | human | 10014 | HDAC5 | histone deacetylase 5 | XM_005256905.2 | 99.7% | 99.7% | 22_24delGCA;355_356insAGC;1779C>G |
| 5 | human | 10014 | HDAC5 | histone deacetylase 5 | XM_011524150.1 | 99.1% | 99.1% | 0_1ins27;1752C>G |
| 6 | human | 10014 | HDAC5 | histone deacetylase 5 | XM_011524149.2 | 99% | 99% | 22_24delGCA;353_354ins27;1755C>G |
| 7 | human | 10014 | HDAC5 | histone deacetylase 5 | XM_017023990.1 | 96.6% | 96.6% | 93_206del;1893C>G |
| 8 | human | 10014 | HDAC5 | histone deacetylase 5 | XM_017023988.1 | 96.6% | 96.5% | 22_24delGCA;96_209del;1896C>G |
| 9 | human | 10014 | HDAC5 | histone deacetylase 5 | XM_017023989.1 | 96.6% | 96.5% | 22_24delGCA;96_209del;1896C>G |
| 10 | human | 10014 | HDAC5 | histone deacetylase 5 | XM_017023991.1 | 96.5% | 96.4% | (many diffs) |
| 11 | human | 10014 | HDAC5 | histone deacetylase 5 | XM_017023996.1 | 96.3% | 96.2% | 0_1ins123;1656C>G |
| 12 | human | 10014 | HDAC5 | histone deacetylase 5 | XM_017023992.1 | 96.1% | 96.1% | (many diffs) |
| 13 | human | 10014 | HDAC5 | histone deacetylase 5 | XM_017023993.1 | 95.8% | 95.7% | (many diffs) |
| 14 | mouse | 15184 | Hdac5 | histone deacetylase 5 | NM_010412.3 | 90.4% | 95.1% | (many diffs) |
| 15 | mouse | 15184 | Hdac5 | histone deacetylase 5 | NM_001077696.1 | 90.4% | 95.1% | (many diffs) |
| 16 | mouse | 15184 | Hdac5 | histone deacetylase 5 | NM_001284250.1 | 89.4% | 94.1% | (many diffs) |
| 17 | mouse | 15184 | Hdac5 | histone deacetylase 5 | XM_017314281.1 | 88.5% | 93% | (many diffs) |
| 18 | mouse | 15184 | Hdac5 | histone deacetylase 5 | NM_001284248.1 | 87.4% | 92% | (many diffs) |
| 19 | mouse | 15184 | Hdac5 | histone deacetylase 5 | XM_011248750.2 | 87.3% | 91.9% | (many diffs) |
| 20 | mouse | 15184 | Hdac5 | histone deacetylase 5 | XM_011248749.2 | 87.2% | 91.8% | (many diffs) |
| 21 | mouse | 15184 | Hdac5 | histone deacetylase 5 | NM_001284249.1 | 87% | 91.8% | (many diffs) |
| 22 | mouse | 15184 | Hdac5 | histone deacetylase 5 | XM_017314282.1 | 87% | 91.8% | (many diffs) |
| 23 | mouse | 15184 | Hdac5 | histone deacetylase 5 | XM_006532254.3 | 85.5% | 89.9% | (many diffs) |
| 24 | mouse | 15184 | Hdac5 | histone deacetylase 5 | XM_011248747.2 | 85.5% | 89.9% | (many diffs) |
| 25 | mouse | 15184 | Hdac5 | histone deacetylase 5 | XM_011248752.2 | 85.4% | 90% | (many diffs) |
| 26 | mouse | 15184 | Hdac5 | histone deacetylase 5 | XM_011248748.2 | 85.4% | 89.8% | (many diffs) |
| 27 | mouse | 15184 | Hdac5 | histone deacetylase 5 | XM_011248754.2 | 84% | 88.6% | (many diffs) |
| 28 | mouse | 15184 | Hdac5 | histone deacetylase 5 | XR_001779878.1 | 62.7% | (many diffs) | |
| 29 | mouse | 15184 | Hdac5 | histone deacetylase 5 | XM_011248755.2 | 61.5% | 64.6% | (many diffs) |
| 30 | mouse | 15184 | Hdac5 | histone deacetylase 5 | XR_001779879.1 | 60.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3435
- ORF length:
- 3366
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gaactctccc aacgagtcgg atgggatgtc aggtcgggaa ccatccttgg 121 aaatcctgcc gcggacttct ctgcacagca tccctgtgac agtggaggtg aagccggtgc 181 tgccaagagc catgcccagt tccatggggg gtgggggtgg aggcagcccc agccctgtgg 241 agctacgggg ggctctggtg ggctctgtgg accccacact gcgggagcag caactgcagc 301 aggagctcct ggcgctcaag cagcagcagc agctgcagaa gcagctcctg ttcgctgagt 361 tccagaaaca gcatgaccac ctgacaaggc agcatgaggt ccagctgcag aagcacctca 421 agcagcagca ggagatgctg gcagccaagc agcagcagga gatgctggca gccaagcggc 481 agcaggagct ggagcagcag cggcagcggg agcagcagcg gcaggaagag ctggagaagc 541 agcggctgga gcagcagctg ctcatcctgc ggaacaagga gaagagcaaa gagagtgcca 601 ttgccagcac tgaggtaaag ctgaggctcc aggaattcct cttgtcgaag tcaaaggagc 661 ccacaccagg cggcctcaac cattccctcc cacagcaccc caaatgctgg ggagcccacc 721 atgcttcttt ggaccagagt tcccctcccc agagcggccc ccctgggacg cctccctcct 781 acaaactgcc tttgcctggg ccctacgaca gtcgagacga cttccccctc cgcaaaacag 841 cctctgaacc caacttgaaa gtgcgttcaa ggctaaaaca gaaggtggct gagcggagaa 901 gcagtcccct cctgcgtcgc aaggatggga ctgttattag cacctttaag aagagagctg 961 ttgagatcac aggtgccggg cctggggcgt cgtccgtgtg taacagcgca cccggctccg 1021 gccccagctc tcccaacagc tcccacagca ccatcgctga gaatggcttt actggctcag 1081 tccccaacat ccccactgag atgctccctc agcaccgagc cctccctctg gacagctccc 1141 ccaaccagtt cagcctctac acgtctcctt ctctgcccaa catctcccta gggctgcagg 1201 ccacggtcac tgtcaccaac tcacacctca ctgcctcccc gaagctgtcg acacagcagg 1261 aggccgagag gcaggccctc cagtccctgc ggcagggtgg cacgctgacc ggcaagttca 1321 tgagcacatc ctctattcct ggctgcctgc tgggcgtggc actggagggc gacgggagcc 1381 cccacgggca tgcctccctg ctgcagcatg tgctgttgct ggagcaggcc cggcagcaga 1441 gcaccctcat tgctgtgcca ctccacgggc agtccccact agtgacgggt gaacgtgtgg 1501 ccaccagcat gcggacggta ggcaagctcc cgcggcatcg gcccctgagc cgcactcagt 1561 cctcaccgct gccgcagagt ccccaggccc tgcagcagct ggtcatgcaa caacagcacc 1621 agcagttcct ggagaagcag aagcagcagc agctacagct gggcaagatc ctcaccaaga 1681 caggggagct gcccaggcag cccaccaccc accctgagga gacagaggag gagctgacgg 1741 agcagcagga ggtcttgctg ggggagggag ccctgaccat gccccgggag ggctccacag 1801 agagtgagag cacacaggaa gacctggagg aggaggacga ggaagaggat ggggaggagg 1861 aggaggattg catccaggtt aaggacgagg agggcgagag tggtgctgag gaggggcccg 1921 acttggagga gcctggtgct ggatacaaaa aactgttctc agatgcccag ccgctgcagc 1981 ctttgcaggt gtaccaggcg cccctcagcc tggccactgt gccccaccag gccctgggcc 2041 gtacccagtc ctcccctgct gcccctgggg gcatgaagag ccccccagac cagcccgtca 2101 agcacctctt caccacaggt gtggtctacg acacgttcat gctaaagcac cagtgcatgt 2161 gcgggaacac acacgtgcac cctgagcatg ctggccggat ccagagcatc tggtcccggc 2221 tgcaggagac aggcctgctt agcaagtgcg agcggatccg aggtcgcaaa gccacgctag 2281 atgagatcca gacagtgcac tctgaatacc acaccctgct ctatgggacc agtcccctca 2341 accggcagaa gctagacagc aagaagttgc tcggccccat cagccagaag atgtatgctg 2401 tgctgccttg tgggggcatc ggggtggaca gtgacaccgt gtggaatgag atgcactcct 2461 ccagtgctgt gcgcatggca gtgggctgcc tgctggagct ggccttcaag gtggctgcag 2521 gagagctcaa gaatggattt gccatcatcc ggcccccagg acaccacgcc gaggaatcca 2581 cagccatggg attctgcttc ttcaactctg tagccatcac cgcaaaactc ctacagcaga 2641 agttgaacgt gggcaaggtc ctcatcgtgg actgggacat tcaccatggc aatggcaccc 2701 agcaggcgtt ctacaatgac ccctctgtgc tctacatctc tctgcatcgc tatgacaacg 2761 ggaacttctt tccaggctct ggggctcctg aagaggttgg tggaggacca ggcgtggggt 2821 acaatgtgaa cgtggcatgg acaggaggtg tggacccccc cattggagac gtggagtacc 2881 ttacagcctt caggacagtg gtgatgccca ttgcccacga gttctcacct gatgtggtcc 2941 tagtctccgc cgggtttgat gctgttgaag gacatctgtc tcctctgggt ggctactctg 3001 tcaccgccag atgttttggc cacttgacca ggcagctgat gaccctggca gggggccggg 3061 tggtgctggc cctggaggga ggccatgact tgaccgccat ctgtgatgcc TCTGAGGCTT 3121 GTGTCTCGGC TCTGCTCAGT GTAGAGCTGC AGCCCTTGGA TGAGGCAGTC TTGCAGCAAA 3181 AGCCCAACAT CAACGCAGTG GCCACGCTAG AGAAAGTCAT CGAGATCCAG AGCAAACACT 3241 GGAGCTGTGT GCAGAAGTTC GCCGCTGGTC TGGGCCGGTC CCTGCGAGAG GCCCAAGCAG 3301 GTGAGACCGA GGAGGCCGAG ACTGTGAGCG CCATGGCCTT GCTGTCGGTG GGGGCCGAGC 3361 AGGCCCAGGC TGCGGCAGCC CGGGAACACA GCCCCAGGCC GGCAGAGGAG CCCATGGAGC 3421 AGGAGCCTGC CCTGTGAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 3481 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 3541 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAGAGCTG ATTCGGCAGC 3601 CTACGGAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt