Construct: ORF TRCN0000489427
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021567.1_s317c1
- DNA Barcode:
- GCTCTTCGTACTCTTATGGTACAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DUSP4 (1846)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489427
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1846 | DUSP4 | dual specificity phosphatase 4 | NM_001394.7 | 99.9% | 100% | 522T>C |
| 2 | human | 1846 | DUSP4 | dual specificity phosphatase 4 | NM_057158.3 | 68.4% | 65.1% | (many diffs) |
| 3 | human | 1846 | DUSP4 | dual specificity phosphatase 4 | XM_011544428.3 | 68.4% | 65.1% | (many diffs) |
| 4 | mouse | 319520 | Dusp4 | dual specificity phosphatase 4 | NM_176933.4 | 89% | 95.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1254
- ORF length:
- 1182
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggtgacg atggaggagc tgcgggagat ggactgcagt gtgctcaaaa 121 ggctgatgaa ccgggacgag aatggcggcg gcgcgggcgg cagcggcagc cacggcaccc 181 tggggctgcc gagcggcggc aagtgcctgc tgctggactg cagaccgttc ctggcgcaca 241 gcgcgggcta catcctaggt tcggtcaacg tgcgctgtaa caccatcgtg cggcggcggg 301 ctaagggctc cgtgagcctg gagcagatcc tgcccgccga ggaggaggta cgcgcccgct 361 tgcgctccgg cctctactcg gcggtcatcg tctacgacga gcgcagcccg cgcgccgaga 421 gcctccgcga ggacagcacc gtgtcgctgg tggtgcaggc gctgcgccgc aacgccgagc 481 gcaccgacat ctgcctgctc aaaggcggct atgagaggtt ttcctccgag tacccagaat 541 tctgttctaa aaccaaggcc ctggcagcca tcccaccccc ggttcccccc agcgccacag 601 agcccttgga cctgggctgc agctcctgtg ggaccccact acacgaccag gggggtcctg 661 tggagatcct tcccttcctc tacctcggca gtgcctacca tgctgcccgg agagacatgc 721 tggacgccct gggcatcacg gctctgttga atgtctcctc ggactgccca aaccactttg 781 aaggacacta tcagtacaag tgcatcccag tggaagataa ccacaaggcc gacatcagct 841 cctggttcat ggaagccata gagtacatcg atgccgtgaa ggactgCCGT GGGCGCGTGC 901 TGGTGCACTG CCAGGCGGGC ATCTCGCGGT CGGCCACCAT CTGCCTGGCC TACCTGATGA 961 TGAAGAAACG GGTGAGGCTG GAGGAGGCCT TCGAGTTCGT TAAGCAGCGC CGCAGCATCA 1021 TCTCGCCCAA CTTCAGCTTC ATGGGGCAGC TGCTGCAGTT CGAGTCCCAG GTGCTGGCCA 1081 CGTCCTGTGC TGCGGAGGCT GCTAGCCCCT CGGGACCCCT GCGGGAGCGG GGCAAGACCC 1141 CCGCCACCCC CACCTCGCAG TTCGTCTTCA GCTTTCCGGT CTCCGTGGGC GTGCACTCGG 1201 CCCCCAGCAG CCTGCCCTAC CTGCACAGCC CCATCACCAC CTCTCCCAGC TGTTAGAACC 1261 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1321 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT CTTGGCTTTA 1381 TATATCTTGT GGAAAGGACG AGCTCTTCGT ACTCTTATGG TACAGACGCG TTAAGTCgac 1441 aatcaacctc tggattacaa aatttgtgaa agatt