Construct: ORF TRCN0000489430

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021414.1_s317c1
DNA Barcode:
CCCAACCGCATTGCTCCAACCAAG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
GPR18 (2841)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489430
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2841 GPR18 G protein-coupled receptor 18 NM_001098200.1 100% 100%
2 human 2841 GPR18 G protein-coupled receptor 18 NM_005292.3 100% 100%
3 human 2841 GPR18 G protein-coupled receptor 18 XM_006719946.3 100% 100%
4 human 2841 GPR18 G protein-coupled receptor 18 XM_024449339.1 100% 100%
5 mouse 110168 Gpr18 G protein-coupled receptor 18 NM_182806.1 82.5% 85.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
75
ORF end:
1068
ORF length:
993
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcgc caccatgatc accctgaaca atcaagatca acctgtccct tttaacagct
121cacatccaga tgaatacaaa attgcagccc ttgtcttcta tagctgtatc ttcataattg
181gattatttgt taacatcact gcattatggg ttttcagttg taccaccaag aagagaacca
241cggtaaccat ctatatgatg aatgtggcat tagtggactt gatatttata atgactttac
301cctttcgaat gttttattat gcaaaagatg aatggccatt tggagagtac ttctgccaga
361ttcttggagc tctcacagtg ttttacccaa gcattgcttt atggcttctt gcctttatta
421gtgctgacag atacatggcc attgtacagc cgaagtacgc caaagaactt aaaaacacgt
481gcaaagccgt gctggcgtgt gtgggagtct ggataatgac cctgaccacg accacccctc
541tgctactgct ctataaagac ccagataaag actccactcc cgccacctgc ctcaagattt
601ctgacatcat ctatctaaaa gctgtgaacg tgctgaacct cactcgactg acattttttt
661tcttgattcc tttgttcatc atgattgggt gctacttggt cattattcat aatctccttc
721acggcaggac gtctaagctg aaacccaaag tcaaggagaa gtccataagg atcatcatca
781cgctgctggt gcaggtgctc gtctgcttta tgcccttcca catctgtttc gctttcctga
841tgctgggaac gggggagaac agttacaatc cctggggagc ctttaccacc ttcctcatga
901acctcagcac gtgtctggat gtgattctct actacatcgt ttcaaaacaa tttcaggctc
961gaGTCATTAG TGTCATGCTA TACCGTAATT ACCTTCGAAG CATGCGCAGA AAAAGTTTCC
1021GATCTGGTAG TCTACGGTCA CTAAGCAATA TAAACAGTGA AATGTTATAG GACCCAGCTT
1081TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1141CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1201CTTGTGGAAA GGACGACCCA ACCGCATTGC TCCAACCAAG ACGCGTTAAG TCgacaatca
1261acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)

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