Construct: ORF TRCN0000489436
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020907.1_s317c1
- DNA Barcode:
- CACCCCAATTTTGCATTAAACCCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADGRE5 (976)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489436
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 976 | ADGRE5 | adhesion G protein-coupled ... | NM_001784.5 | 100% | 100% | |
| 2 | human | 976 | ADGRE5 | adhesion G protein-coupled ... | NM_001025160.2 | 94.4% | 94.4% | 345_476del |
| 3 | human | 976 | ADGRE5 | adhesion G protein-coupled ... | NM_078481.4 | 88.8% | 88.8% | 344_622del |
| 4 | human | 976 | ADGRE5 | adhesion G protein-coupled ... | XM_011528451.3 | 87.8% | 87.7% | 344_622del;2479_2480delAAinsGC;2481_2482ins24 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2298
- ORF length:
- 2226
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggaggc cgcgtctttc tcgcattctg tgtctggctg actctgccgg 121 gagctgaaac ccaggactcc aggggctgtg cccggtggtg ccctcagaac tcctcgtgtg 181 tcaatgccac cgcctgtcgc tgcaatccag ggttcagctc tttttctgag atcatcacca 241 ccccgacgga gacttgtgac gacatcaacg agtgtgcaac accgtcgaaa gtgtcatgcg 301 gaaaattctc ggactgctgg aacacagagg ggagctacga ctgcgtgtgc agcccgggat 361 atgagcctgt ttctggggca aaaacattca agaatgagag cgagaacacc tgtcaagatg 421 tggacgagtg cagctccggg cagcatcagt gtgacagctc caccgtctgc ttcaacaccg 481 tgggttcata cagctgccgc tgccgcccag gctggaagcc cagacacgga atcccgaata 541 accaaaagga cactgtctgt gaagatatga ctttctccac ctggaccccg ccccctggag 601 tccacagcca gacgctttcc cgattcttcg acaaagtcca ggacctgggc agagactcca 661 agacaagctc agccgaggtc accatccaga atgtcatcaa attggtggat gaactgatgg 721 aagctcctgg agacgtagag gccctggcgc cacctgtccg gcacctcata gccacccagc 781 tgctctcaaa ccttgaagat atcatgagga tcctggccaa gagcctgcct aaaggcccct 841 tcacctacat ttccccttcg aacacagagc tgaccctgat gatccaggag cggggggaca 901 agaacgtcac tatgggtcag agcagcgcac gcatgaagct gaattgggct gtggcagctg 961 gagccgagga tccaggcccc gccgtggcgg gcatcctctc catccagaac atgacgacat 1021 tgctggccaa tgcctccttg aacctgcatt ccaagaagca agccgaactg gaggagatat 1081 atgaaagcag catccgtggt gtccaactca gacgcctctc tgccgtcaac tccatctttc 1141 tgagccacaa caacaccaag gaactcaact cccccatcct tttcgccttc tcccaccttg 1201 agtcctccga tggggaggcg ggaagagacc ctcctgccaa ggacgtgatg cctgggccac 1261 ggcaggagct gctctgtgcc ttctggaaga gtgacagcga caggggaggg cactgggcca 1321 ccgagggctg ccaggtgctg ggcagcaaga acggcagcac cacctgccaa tgcagccacc 1381 tgagcagctt tgcgatcctt atggctcatt atgacgtgga ggactggaag ctgaccctga 1441 tcaccagggt gggactggcg ctgtcactct tctgcctgct gctgtgcatc ctcactttcc 1501 tgctggtgcg gcccatccag ggctcgcgca ccaccataca cctgcacctc tgcatctgcc 1561 tcttcgtggg ctccaccatc ttcctggccg gcatcgagaa cgaaggcggc caggtggggc 1621 tgcgctgccg cctggtggcc gggctgctgc actactgttt cctggccgcc ttctgctgga 1681 tgagcctcga aggcctggag ctctactttc ttgtggtgcg cgtgttccaa ggccagggcc 1741 tgagtacgcg ctggctctgc ctgatcggct atggcgtgcc cctgctcatc gtgggcgtct 1801 cggctgccat ctacagcaag ggctacggcc gccccagata ctgctggttg gactttgagc 1861 agggcttcct ctggagcttc ttgggacctg tgaccttcAT CATTTTGTGC AATGCTGTCA 1921 TTTTCGTGAC TACCGTCTGG AAGCTCACTC AGAAGTTTTC TGAAATCAAT CCAGACATGA 1981 AGAAATTAAA GAAGGCGAGG GCGCTGACCA TCACGGCCAT CGCGCAGCTC TTCCTGTTGG 2041 GCTGCACCTG GGTCTTTGGC CTGTTCATCT TCGACGATCG GAGCTTGGTG CTGACCTATG 2101 TGTTTACCAT CCTCAACTGC CTGCAGGGCG CCTTCCTCTA CCTGCTGCAC TGCCTGCTCA 2161 ACAAGAAGGT TCGGGAAGAA TACCGGAAGT GGGCCTGCCT AGTTGCTGGG GGGAGCAAGT 2221 ACTCAGAATT CACCTCCACC ACGTCTGGCA CTGGCCACAA TCAGACCCGG GCCCTCAGGG 2281 CATCAGAGTC CGGCATATAG GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2341 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2401 AAAGTATTTC GTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACACCCC AATTTTGCAT 2461 TAAACCCTAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt