Construct: ORF TRCN0000489442
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019301.1_s317c1
- DNA Barcode:
- GACCACGCCGGGTCAAAAACTGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR158 (57512)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489442
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57512 | GPR158 | G protein-coupled receptor 158 | NM_020752.3 | 76.3% | 99.9% | (many diffs) |
| 2 | human | 57512 | GPR158 | G protein-coupled receptor 158 | XM_017016452.2 | 43.3% | 57.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 3723
- ORF length:
- 3648
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgggc gccatggcct accccctgct gctgtgtctg ctgctggccc 121 agctcggact gggagccgtg ggcgccagca gagatcctca gggcagaccc gacagcccca 181 gagagagaac ccccaagggc aagccccacg cccagcagcc tggcagagcc agcgccagcg 241 atagcagcgc cccctggtcc agaagcaccg acggcacaat cctggcccag aaactggccg 301 aagaggtgcc catggacgtg gccagctacc tgtacaccgg cgacagccac cagctgaagc 361 gggccaactg cagcggcaga tacgagctgg ccggcctgcc tggaaagtgg cctgccctgg 421 ccagcgccca ccctagtctg cacagagccc tggacaccct gacccacgcc accaactttc 481 tgaacgtgat gctgcagagc aacaagagca gagagcagaa cctgcaggac gacctggact 541 ggtatcaggc cctcgtgtgg tccctgctgg aaggcgagcc cagcatcagc agagccgcca 601 tcaccttcag caccgacagc ctgagcgccc cagcccctca ggtgttcctg caggccaccc 661 gggaagagag ccggattctc ctccaggatc tctcttcctc cgcccctcac ctggccaatg 721 ccaccctgga aaccgagtgg ttccacggcc tgcggcggaa gtggcggcca catctgcaca 781 gaagaggacc caaccagggc cctagaggcc tgggacacag ctggcggaga aaggatggcc 841 tgggcggcga caagagccac ttcaagtggt ccccccccta cctggaatgc gagaacggca 901 gctacaagcc cggctggctg gtcacactga gcagcgccat ctacggcctg cagcccaacc 961 tggtgcccga gttccggggc gtgatgaagg tggacatcaa cctgcagaaa gtggacatcg 1021 accagtgcag cagcgacggc tggttcagcg gcacccacaa gtgccacctg aacaacagcg 1081 agtgcatgcc catcaagggc ctgggcttcg tgctgggcgc ctacgagtgc atctgcaagg 1141 ccggcttcta ccaccccggc gtgctgcccg tgaacaactt cagacgcaga ggccccgatc 1201 agcacatcag cggcagcacc aaggacgtgt ccgaagaggc ctacgtgtgc ctgccctgca 1261 gagagggctg ccccttctgc gccgacgaca gcccatgctt cgtgcaggaa gataagtacc 1321 tgcggctggc catcatcagt tttcaggccc tgtgcatgct gctggacttc gtgtccatgc 1381 tggtggtgta ccacttccgg aaggccaaga gcatccgggc cagcggcctg atcctgctgg 1441 aaaccatcct gttcggctca ctgctcctgt acttccccgt ggtcatcctg tacttcgagc 1501 cctccacctt ccggtgcatc ctgctgagat gggccagact gctgggcttc gccaccgtgt 1561 acggcaccgt gaccctgaag ctgcaccggg tgctgaaagt gttcctgagc cggaccgccc 1621 agcggatccc ctacatgaca ggcggcagag tgatgcggat gctggctgtg atcctcctgg 1681 tcgtgttctg gttcctgatc ggctggacca gcagcgtgtg ccagaacctg gaaaagcaga 1741 tcagcctgat cggccagggc aagaccagcg accacctgat cttcaacatg tgcctgatcg 1801 accgctggga ctacatgacc gccgtggccg agttcctgtt cctgctgtgg ggcgtgtacc 1861 tgtgctacgc cgtgcggaca gtgcccagcg cctttcacga gccccggtat atggccgtgg 1921 ccgtgcacaa cgagctgatc atctccgcca tcttccacac catcagattc gtgctcgctt 1981 ctagactgca gagcgactgg atgctgatgc tgtactttgc ccacacccac ctgaccgtga 2041 ccgtgacaat cggcctgctg ctgatcccca agttcagcca cagcagcaac aacccccggg 2101 acgatatcgc caccgaggcc tacgaggacg agctggacat gggccgcagc ggcagctacc 2161 tgaacagcag catcaacagc gcttggagcg agcacagcct ggaccccgag gacatccggg 2221 acgagctgaa gaagctgtac gcccagctgg aaatctacaa gcggaagaag atgatcacca 2281 acaaccctca cctccagaag aagcgctgca gcaagaaggg actgggccgg tccatcatgc 2341 ggcggatcac cgagatcccc gagacagtgt cccggcagtg cagcaaagag gacaaagagg 2401 gcgccgacca cggcaccgcc aagggaacag ccctgatccg gaagaacccc cccgagagca 2461 gcggcaacac cggcaagtcc aaagaggaaa ccctgaagaa ccgggtgttc agcctgaaga 2521 agtcccacag cacctacgac cacgtgcggg accagaccga ggaaagcagc agcctgccca 2581 ccgagagcca ggaagaggaa acaaccgaga acagcaccct ggaatccctg agcggcaaga 2641 agctgaccca gaagctgaaa gaggacagcg aggccgagag caccgagagc gtgcccctcg 2701 tgtgcaagag cgcctccgcc cacaacctgt ccagcgagaa gaaaaccggc cacccccgga 2761 ccagcatgct gcagaaaagc ctgagcgtga tcgccagcgc caaagaaaag accctcggcc 2821 tggctggcaa gactcagaca gccggcgtgg aagagaggac caagagccag aagcccctgc 2881 ccaaggacaa agaaacaaac cggaaccaca gcaatagcga caacaccgag acaaaggacc 2941 ctgcccccca gaacagcaac cccgccgagg aaccccggaa gccccagaaa agcggcatca 3001 tgaagcagca gcgcgtgaac cccaccaccg ccaacagcga cctgaaccct ggcaccaccc 3061 agatgaagga caacttcgac atcggcgaag tgtgcccctg ggaggtgtac gacctgaccc 3121 caggccccgt gcccagcgag agcaaggtgc agaaacacgt gtccatcgtg gccagcgaga 3181 tggaaaagaa ccccaccttt agcctgaaag agaagtctca ccacaagccc aaggccgctg 3241 aagtgtgcca gcagagcaac cagaagcgga tcgacaaggc cgaagtctgc ctgtgggaga 3301 gccagggcca gagcatcctg gaagatgaga agctgctgat tagcaagacc cccgtgctgc 3361 cTGAGCGGGC CAAAGAAGAG AACGGCGGCC AGCCCAGGGC CGCCAATGTC TGTGCCGGCC 3421 AGAGCGAGGA ACTGCCCCCT AAAGCCGTGG CCAGCAAGAC CGAGAACGAG AACCTGAACC 3481 AGATCGGCCA CCAGGAAAAG AAAACCAGCA GCAGCGAGGA AAACGTGCGG GGCTCCTACA 3541 ACAGCTCCAA CAACTTCCAG CAGCCCCTGA CCAGCAGGGC TGAAGTCTGT CCATGGGAAT 3601 TCGAGACACC CGCCCAGCCC AACGCCGGCA GATCTGTGGC CCTGCCTGCC AGCTCTGCCC 3661 TGAGCGCCAA CAAGATCGCC GGACCCCGGA AAGAGGAAAT CTGGGACAGC TTCAAAGTGG 3721 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 3781 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 3841 TTTATATATC TTGTGGAAAG GACGAGACCA CGCCGGGTCA AAAACTGCAA CGCGTTAAGT 3901 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt