Construct: ORF TRCN0000489453
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021972.1_s317c1
- DNA Barcode:
- TGTAAGTCCAGCGACTTTAGCCAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CYSLTR1 (10800)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489453
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10800 | CYSLTR1 | cysteinyl leukotriene recep... | NM_001282186.1 | 99.9% | 100% | 927C>T |
| 2 | human | 10800 | CYSLTR1 | cysteinyl leukotriene recep... | NM_001282187.2 | 99.9% | 100% | 927C>T |
| 3 | human | 10800 | CYSLTR1 | cysteinyl leukotriene recep... | NM_001282188.2 | 99.9% | 100% | 927C>T |
| 4 | human | 10800 | CYSLTR1 | cysteinyl leukotriene recep... | NM_006639.4 | 99.9% | 100% | 927C>T |
| 5 | mouse | 58861 | Cysltr1 | cysteinyl leukotriene recep... | NM_001281862.1 | 87.2% | 87% | (many diffs) |
| 6 | mouse | 58861 | Cysltr1 | cysteinyl leukotriene recep... | NM_001281859.1 | 83.9% | 83.8% | (many diffs) |
| 7 | mouse | 58861 | Cysltr1 | cysteinyl leukotriene recep... | NM_021476.5 | 83.9% | 83.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1080
- ORF length:
- 1011
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggatgaaaca ggaaatctga cagtatcttc tgccacatgc catgacacta 121 ttgatgactt ccgcaatcaa gtgtattcca ccttgtactc tatgatctct gttgtaggct 181 tctttggcaa tggctttgtg ctctatgtcc tcataaaaac ctatcacaag aagtcagcct 241 tccaagtata catgattaat ttagcagtag cagatctact ttgtgtgtgc acactgcctc 301 tccgtgtggt ctattatgtt cacaaaggca tttggctctt tggtgacttc ttgtgccgcc 361 tcagcaccta tgctttgtat gtcaacctct attgtagcat cttctttatg acagccatga 421 gctttttccg gtgcattgca attgtttttc cagtccagaa cattaatttg gttacacaga 481 aaaaagccag gtttgtgtgt gtaggtattt ggatttttgt gattttgacc agttctccat 541 ttctaatggc caaaccacaa aaagatgaga aaaataatac caagtgcttt gagcccccac 601 aagacaatca aactaaaaat catgttttgg tcttgcatta tgtgtcattg tttgttggct 661 ttatcatccc ttttgttatt ataattgtct gttacacaat gatcattttg aCCTTACTAA 721 AAAAATCAAT GAAAAAAAAT CTGTCAAGTC ATAAAAAGGC TATAGGAATG ATCATGGTCG 781 TGACCGCTGC CTTTTTAGTC AGTTTCATGC CATATCATAT TCAACGTACC ATTCACCTTC 841 ATTTTTTACA CAATGAAACT AAACCCTGTG ATTCTGTCCT TAGAATGCAG AAGTCCGTGG 901 TCATAACCTT GTCTCTGGCT GCATCCAATT GTTGCTTTGA CCCTCTCCTA TATTTCTTTT 961 CTGGGGGTAA CTTTAGGAAA AGGCTGTCTA CATTTAGAAA GCATTCTTTG TCCAGCGTGA 1021 CTTATGTACC CAGAAAGAAG GCCTCTTTGC CAGAAAAAGG AGAAGAAATA TGTAAAGTAT 1081 AGAACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1141 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1201 GGCTTTATAT ATCTTGTGGA AAGGACGATG TAAGTCCAGC GACTTTAGCC AGACGCGTTA 1261 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt