Construct: ORF TRCN0000489459
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021328.1_s317c1
- DNA Barcode:
- AGCAATTTTCTACGCTTTAGGGGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- AIF1L (83543)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489459
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83543 | AIF1L | allograft inflammatory fact... | NM_001185096.1 | 100% | 100% | |
| 2 | human | 83543 | AIF1L | allograft inflammatory fact... | NM_031426.4 | 67.7% | 66% | (many diffs) |
| 3 | human | 83543 | AIF1L | allograft inflammatory fact... | NM_001185095.1 | 57.7% | 56.2% | (many diffs) |
| 4 | human | 83543 | AIF1L | allograft inflammatory fact... | XM_017015177.1 | 53.8% | 44.7% | (many diffs) |
| 5 | human | 83543 | AIF1L | allograft inflammatory fact... | NR_033701.1 | 8.7% | (many diffs) | |
| 6 | mouse | 108897 | Aif1l | allograft inflammatory fact... | NM_145144.1 | 62.7% | 62.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 399
- ORF length:
- 306
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGCAGGCA CCATGTCGGG CGAGCTCAGC AACAGGTTCC 121 AAGGAGGGAA GGCGTTCGGC TTGCTCAAAG CCCGGCAGGA GAGGAGGCTG GCCGAGATCA 181 ACCGGGAGTT TCTGTGTGAC CAGAAGTACA GTGATGAAGA GAACCTTCCA GAAAAGCTCA 241 CAGCCTTCAA AGAGAAGTAC ATGGAGTTTG ACCTGAACAA TGAAGGCGAG ATTGACCTGA 301 TGTCTTTAAA GAGGATGATG GAGAAGCTTG GTGTCCCCAA GACCCACCTG GAGATGAAGA 361 AGATGATCTC AGAGGTGACA GGAGGGAGTC ATGATGTTTA GAACCCAGCT TTCTTGTACA 421 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 481 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 541 AGGACGAAGC AATTTTCTAC GCTTTAGGGG CACGCGTTAA GTCgacaatc aacctctgga 601 ttacaaaatt tgtgaaagat t