Construct: ORF TRCN0000489473
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019143.1_s317c1
- DNA Barcode:
- ACGTCGGTTGGTGTTTGACACTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDR2 (4921)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489473
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4921 | DDR2 | discoidin domain receptor t... | NM_001014796.3 | 99.9% | 99.8% | 1260C>G;2565_2566insG |
| 2 | human | 4921 | DDR2 | discoidin domain receptor t... | NM_001354982.2 | 99.9% | 99.8% | 1260C>G;2565_2566insG |
| 3 | human | 4921 | DDR2 | discoidin domain receptor t... | NM_001354983.2 | 99.9% | 99.8% | 1260C>G;2565_2566insG |
| 4 | human | 4921 | DDR2 | discoidin domain receptor t... | NM_006182.4 | 99.9% | 99.8% | 1260C>G;2565_2566insG |
| 5 | human | 4921 | DDR2 | discoidin domain receptor t... | XM_011509587.2 | 99.9% | 99.8% | 1260C>G;2565_2566insG |
| 6 | human | 4921 | DDR2 | discoidin domain receptor t... | XM_011509588.3 | 62.7% | 58.8% | (many diffs) |
| 7 | mouse | 18214 | Ddr2 | discoidin domain receptor f... | NM_022563.2 | 90.1% | 95.7% | (many diffs) |
| 8 | mouse | 18214 | Ddr2 | discoidin domain receptor f... | XM_006496696.3 | 90.1% | 95.7% | (many diffs) |
| 9 | mouse | 18214 | Ddr2 | discoidin domain receptor f... | XM_006496697.3 | 90.1% | 95.7% | (many diffs) |
| 10 | mouse | 18214 | Ddr2 | discoidin domain receptor f... | XM_006496698.3 | 90.1% | 95.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2640
- ORF length:
- 2568
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgatcctg attcccagaa tgctcttggt gctgttcctg ctgctgccta 121 tcttgagttc tgcaaaagct caggttaatc cagctatatg ccgctatcct ctgggcatgt 181 caggaggcca gattccagat gaggacatca cagcttccag tcagtggtca gagtccacag 241 ctgccaaata tggaaggctg gactcagaag aaggggatgg agcctggtgc cctgagattc 301 cagtggaacc tgatgacctg aaggagtttc tgcagattga cttgcacacc ctccatttta 361 tcactctggt ggggacccag gggcgccatg caggaggtca tggcatcgag tttgccccca 421 tgtacaagat caattacagt cgggatggca ctcgctggat ctcttggcgg aaccgtcatg 481 ggaaacaggt gctggatgga aatagtaacc cctatgacat tttcctaaag gacttggagc 541 cgcccattgt agccagattt gtccggttca ttccagtcac cgaccactcc atgaatgtgt 601 gtatgagagt ggagctttac ggctgtgtct ggctagatgg cttggtgtct tacaatgctc 661 cagctgggca gcagtttgta ctccctggag gttccatcat ttatctgaat gattctgtct 721 atgatggagc tgttggatac agcatgacag aagggctagg ccaattgacc gatggtgtgt 781 ctggcctgga cgatttcacc cagacccatg aataccacgt gtggcccggc tatgactatg 841 tgggctggcg gaacgagagt gccaccaatg gctacattga gatcatgttt gaatttgacc 901 gcatcaggaa tttcactacc atgaaggtcc actgcaacaa catgtttgct aaaggtgtga 961 agatctttaa ggaggtacag tgctacttcc gctctgaagc cagtgagtgg gaacctaatg 1021 ccatttcctt cccccttgtc ctggatgacg tcaaccccag tgctcggttt gtcacggtgc 1081 ctctccacca ccgaatggcc agtgccatca agtgtcaata ccattttgca gatacctgga 1141 tgatgttcag tgagatcacc ttccaatcag atgctgcaat gtacaacaac tctgaagccc 1201 tgcccacctc tcctatggca cccacaacct atgatccaat gcttaaagtt gatgacagca 1261 acactcggat cctgattggc tgcttggtgg ccatcatctt tatcctcctg gccatcattg 1321 tcatcatcct gtggaggcag ttctggcaga aaatgctgga gaaggcttct cggaggatgc 1381 tggatgatga aatgacagtc agcctttccc tgccaagtga ttctagcatg ttcaacaata 1441 accgctcctc atcacctagt gaacaagggt ccaactcgac ttacgatcgc atctttcccc 1501 ttcgccctga ctaccaggag ccatccaggc tgatacgaaa actcccagaa tttgctccag 1561 gggaggagga gtcaggctgc agcggtgttg tgaagccagt ccagcccagt ggccctgagg 1621 gggtgcccca ctatgcagag gctgacatag tgaacctcca aggagtgaca ggaggcaaca 1681 catactcagt gcctgccgtc accatggacc tgctctcagg aaaagatgtg gctgtggagg 1741 agttccccag gaaactccta actttcaaag agaagctggg agaaggacag tttggggagg 1801 ttcatctctg tgaagtggag ggaatggaaa aattcaaaga caaagatttt gccctagatg 1861 tcagtgccaa ccagcctgtc ctggtggctg tgaaaatgct ccgagcagat gccaacaaga 1921 atgccaggaa tgattttctt aaggagataa agatcatgtc tcggctcaag gacccaaaca 1981 tcatccatct attagctgtg tgtatcactg atgaccctct ctgtatgatc actgaataca 2041 tggagaatgg agatctcaat cagtttcttt cccgccacga gccccctaat tcttcctcca 2101 gcgatgtacg cactgtcagt tacaccaatc tgaagtttat ggctacccaa attgcctctg 2161 gcatgaagta cctttcctct cttaattttg ttcaccgaga tctggccaca cgaaactgtt 2221 tagtgggtaa gaactacaca atcaagatag ctgactttgg aatgagcagg aacctgtaca 2281 gtggtgacta ttaccggatC CAGGGCCGGG CAGTGCTCCC TATCCGCTGG ATGTCTTGGG 2341 AGAGTATCTT GCTGGGCAAG TTCACTACAG CAAGTGATGT GTGGGCCTTT GGGGTTACTT 2401 TGTGGGAGAC TTTCACCTTT TGTCAAGAAC AGCCCTATTC CCAGCTGTCA GATGAACAGG 2461 TTATTGAGAA TACTGGAGAG TTCTTCCGAG ACCAAGGGAG GCAGACTTAC CTCCCTCAAC 2521 CAGCCATTTG TCCTGACTCT GTGTATAAGC TGATGCTCAG CTGCTGGAGA AGAGATACGA 2581 AGAACCGTCC CTCATTCCAA GAAATCCACC TTCTGCTCCT TCAACAAGGC GACGAGGACC 2641 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2701 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2761 ATATATCTTG TGGAAAGGAC GAACGTCGGT TGGTGTTTGA CACTCAACGC GTTAAGTCga 2821 caatcaacct ctggattaca aaatttgtga aagatt