Construct: ORF TRCN0000489487
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021710.1_s317c1
- DNA Barcode:
- GTTCCAAAAGCTGGTGCGCGAGGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- KDR (3791)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489487
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3791 | KDR | kinase insert domain receptor | NM_002253.3 | 40.6% | 40.6% | 1_2415del |
| 2 | mouse | 16542 | Kdr | kinase insert domain protei... | XM_011240817.2 | 41.9% | 44.2% | (many diffs) |
| 3 | mouse | 16542 | Kdr | kinase insert domain protei... | NM_010612.2 | 36% | 37.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 81
- ORF end:
- 1734
- ORF length:
- 1653
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctactt gtccatcgtc atggatccag atgaactccc attggatgaa cattgtgaac 121 gactgcctta tgatgccagc aaatgggaat tccccagaga ccggctgaag ctaggtaagc 181 ctcttggccg tggtgccttt ggccaagtga ttgaagcaga tgcctttgga attgacaaga 241 cagcaacttg caggacagta gcagtcaaaa tgttgaaaga aggagcaaca cacagtgagc 301 atcgagctct catgtctgaa ctcaagatcc tcattcatat tggtcaccat ctcaatgtgg 361 tcaaccttct aggtgcctgt accaagccag gagggccact catggtgatt gtggaattct 421 gcaaatttgg aaacctgtcc acttacctga ggagcaagag aaatgaattt gtcccctaca 481 agaccaaagg ggcacgattc cgtcaaggga aagactacgt tggagcaatc cctgtggatc 541 tgaaacggcg cttggacagc atcaccagta gccagagctc agccagctct ggatttgtgg 601 aggagaagtc cctcagtgat gtagaagaag aggaagctcc tgaagatctg tataaggact 661 tcctgacctt ggagcatctc atctgttaca gcttccaagt ggctaagggc atggagttct 721 tggcatcgcg aaagtgtatc cacagggacc tggcggcacg aaatatcctc ttatcggaga 781 agaacgtggt taaaatctgt gactttggct tggcccggga tatttataaa gatccagatt 841 atgtcagaaa aggagatgct cgcctccctt tgaaatggat ggccccagaa acaatttttg 901 acagagtgta cacaatccag agtgacgtct ggtcttttgg tgttttgctg tgggaaatat 961 tttccttagg tgcttctcca tatcctgggg taaagattga tgaagaattt tgtaggcgat 1021 tgaaagaagg aactagaatg agggcccctg attatactac accagaaatg taccagacca 1081 tgctggactg ctggcacggg gagcccagtc agagacccac gttttcagag ttggtggaac 1141 atttgggaaa tctcttgcaa gctaatgctc agcaggatgg caaagactac attgttcttc 1201 cgatatcaga gactttgagc atggaagagg attctggact ctctctgcct acctcacctg 1261 tttcctgtat ggaggaggag gaagtatgtg accccaaatt ccattatgac aacacagcag 1321 gaaTCAGTCA GTATCTGCAG AACAGTAAGC GAAAGAGCCG GCCTGTGAGT GTAAAAACAT 1381 TTGAAGATAT CCCGTTAGAA GAACCAGAAG TAAAAGTAAT CCCAGATGAC AACCAGACGG 1441 ACAGTGGTAT GGTTCTTGCC TCAGAAGAGC TGAAAACTTT GGAAGACAGA ACCAAATTAT 1501 CTCCATCTTT TGGTGGAATG GTGCCCAGCA AAAGCAGGGA GTCTGTGGCA TCTGAAGGCT 1561 CAAACCAGAC AAGCGGCTAC CAGTCCGGAT ATCACTCCGA TGACACAGAC ACCACCGTGT 1621 ACTCCAGTGA GGAAGCAGAA CTTTTAAAGC TGATAGAGAT TGGAGTGCAA ACCGGTAGCA 1681 CAGCCCAGAT TCTCCAGCCT GACTCGGGGA CCACACTGAG CTCTCCTCCT GTTTAAGACC 1741 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1801 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1861 ATATATCTTG TGGAAAGGAC GAGTTCCAAA AGCTGGTGCG CGAGGTACGC GTTAAGTCga 1921 caatcaacct ctggattaca aaatttgtga aagatt