Construct: ORF TRCN0000489493
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020678.1_s317c1
- DNA Barcode:
- TGTACGGGCGCCCAGCGATACCAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TTK (7272)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489493
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7272 | TTK | TTK protein kinase | NM_003318.5 | 99.9% | 100% | 1581A>T;2367A>C |
| 2 | human | 7272 | TTK | TTK protein kinase | XM_011536099.3 | 99.9% | 100% | 1581A>T;2367A>C |
| 3 | human | 7272 | TTK | TTK protein kinase | NM_001166691.1 | 99.8% | 99.8% | 1255_1256insAGC;1578A>T;2364A>C |
| 4 | human | 7272 | TTK | TTK protein kinase | XM_011536100.3 | 99.8% | 99.8% | 1255_1256insAGC;1578A>T;2364A>C |
| 5 | human | 7272 | TTK | TTK protein kinase | XM_017011242.2 | 93.5% | 93.5% | 0_1ins165;1416A>T;2202A>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2643
- ORF length:
- 2571
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaatcc gaggatttaa gtggcagaga attgacaatt gattccataa 121 tgaacaaagt gagagacatt aaaaataagt ttaaaaatga agaccttact gatgaactaa 181 gcttgaataa aatttctgct gatactacag ataactcggg aactgttaac caaattatga 241 tgatggcaaa caacccagag gactggttga gtttgttgct caaactagag aaaaacagtg 301 ttccgctaag tgatgctctt ttaaataaat tgattggtcg ttacagtcaa gcaattgaag 361 cgcttccccc agataaatat ggccaaaatg agagttttgc tagaattcaa gtgagatttg 421 ctgaattaaa agctattcaa gagccagatg atgcacgtga ctactttcaa atggccagag 481 caaactgcaa gaaatttgct tttgttcata tatcttttgc acaatttgaa ctgtcacaag 541 gtaatgtcaa aaaaagtaaa caacttcttc aaaaagctgt agaacgtgga gcagtaccac 601 tagaaatgct ggaaattgcc ctgcggaatt taaacctcca aaaaaagcag ctgctttcag 661 aggaggaaaa gaagaattta tcagcatcta cggtattaac tgcccaagaa tcattttccg 721 gttcacttgg gcatttacag aataggaaca acagttgtga ttccagagga cagactacta 781 aagccaggtt tttatatgga gagaacatgc caccacaaga tgcagaaata ggttaccgga 841 attcattgag acaaactaac aaaactaaac agtcatgccc atttggaaga gtcccagtta 901 accttctaaa tagcccagat tgtgatgtga agacagatga ttcagttgta ccttgtttta 961 tgaaaagaca aacctctaga tcagaatgcc gagatttggt tgtgcctgga tctaaaccaa 1021 gtggaaatga ttcctgtgaa ttaagaaatt taaagtctgt tcaaaatagt catttcaagg 1081 aacctctggt gtcagatgaa aagagttctg aacttattat tactgattca ataaccctga 1141 agaataaaac ggaatcaagt cttctagcta aattagaaga aactaaagag tatcaagaac 1201 cagaggttcc agagagtaac cagaaacagt ggcaatctaa gagaaagtca gagtgtatta 1261 accagaatcc tgctgcatct tcaaatcact ggcagattcc ggagttagcc cgaaaagtta 1321 atacagagca gaaacatacc acttttgagc aacctgtctt ttcagtttca aaacagtcac 1381 caccaatatc aacatctaaa tggtttgacc caaaatctat ttgtaagaca ccaagcagca 1441 ataccttgga tgattacatg agctgtttta gaactccagt tgtaaagaat gactttccac 1501 ctgcttgtca gttgtcaaca ccttatggcc aacctgcctg tttccagcag caacagcatc 1561 aaatacttgc cactccactt caaaatttac aggttttagc atcttcttca gcaaatgaat 1621 gcatttcggt taaaggaaga atttattcca ttttaaagca gataggaagt ggaggttcaa 1681 gcaaggtatt tcaggtgtta aatgaaaaga aacagatata tgctataaaa tatgtgaact 1741 tagaagaagc agataaccaa actcttgata gttaccggaa cgaaatagct tatttgaata 1801 aactacaaca acacagtgat aagatcatcc gactttatga ttatgaaatc acggaccagt 1861 acatctacat ggtaatggag tgtggaaata ttgatcttaa tagttggctt aaaaagaaaa 1921 aatccattga tccatgggaa cgcaagagtt actggaaaaa tatgttagag gcagttcaca 1981 caatccatca acatggcatt gttcacagtg atcttaaacc agctaacttt ctgatagttg 2041 atggaatgct aaagctaatt gattttggga ttgcaaacca aatgcaacca gatacaacaa 2101 gtgttgttaa agattctcag gttggcacag ttaattatat gccaccagaa gcaatcaaag 2161 atatgtcttc ctccagagag aatgggaaat ctaagtcaaa gataagcccc aaaagtgatg 2221 tttggtcctt aggatgtatt ttgtactata tgacttacgg gaaaacacca tttcagcaga 2281 taattaatca gatttcTAAA TTACATGCCA TAATTGATCC TAATCATGAA ATTGAATTTC 2341 CCGATATTCC AGAGAAAGAT CTTCAAGATG TGTTAAAGTG TTGTTTAAAA AGGGACCCAA 2401 AACAGAGGAT ATCCATTCCT GAGCTCCTGG CTCATCCCTA TGTTCAAATT CAAACTCATC 2461 CAGTTAACCA AATGGCCAAG GGAACCACTG AAGAAATGAA ATATGTTCTG GGCCAACTTG 2521 TTGGTCTGAA TTCTCCTAAC TCCATTTTGA AAGCTGCTAA AACTTTATAT GAACACTATA 2581 GTGGTGGTGA AAGTCATAAT TCTTCATCCT CCAAGACTTT TGAAAAAAAA AGGGGAAAAA 2641 AATAGAACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2701 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2761 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATGTACGGGC GCCCAGCGAT ACCAGACGCG 2821 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt