Construct: ORF TRCN0000489496
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020243.1_s317c1
- DNA Barcode:
- ATTAGTCCATTGAATGAACGATAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RPS6KA6 (27330)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489496
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27330 | RPS6KA6 | ribosomal protein S6 kinase A6 | NM_014496.5 | 100% | 100% | |
| 2 | human | 27330 | RPS6KA6 | ribosomal protein S6 kinase A6 | NM_001330512.1 | 96.4% | 94.7% | (many diffs) |
| 3 | human | 27330 | RPS6KA6 | ribosomal protein S6 kinase A6 | XM_011530917.2 | 96.4% | 94.7% | (many diffs) |
| 4 | human | 27330 | RPS6KA6 | ribosomal protein S6 kinase A6 | XM_017029423.2 | 94.8% | 92.6% | (many diffs) |
| 5 | human | 27330 | RPS6KA6 | ribosomal protein S6 kinase A6 | XM_017029424.1 | 94.8% | 92.6% | (many diffs) |
| 6 | human | 27330 | RPS6KA6 | ribosomal protein S6 kinase A6 | XM_017029425.1 | 94.8% | 92.6% | (many diffs) |
| 7 | human | 27330 | RPS6KA6 | ribosomal protein S6 kinase A6 | XM_011530919.2 | 86.1% | 86.1% | 0_1ins309 |
| 8 | human | 27330 | RPS6KA6 | ribosomal protein S6 kinase A6 | XM_011530920.2 | 83.7% | 83.7% | 0_1ins363 |
| 9 | human | 27330 | RPS6KA6 | ribosomal protein S6 kinase A6 | XM_017029426.1 | 67.9% | 67.9% | 0_1ins717 |
| 10 | mouse | 67071 | Rps6ka6 | ribosomal protein S6 kinase... | XM_011247707.2 | 85.5% | 85.3% | (many diffs) |
| 11 | mouse | 67071 | Rps6ka6 | ribosomal protein S6 kinase... | XM_011247706.2 | 83.6% | 83.3% | (many diffs) |
| 12 | mouse | 67071 | Rps6ka6 | ribosomal protein S6 kinase... | XM_006528404.2 | 83.1% | 83.8% | (many diffs) |
| 13 | mouse | 67071 | Rps6ka6 | ribosomal protein S6 kinase... | XM_006528403.2 | 77.4% | 78% | (many diffs) |
| 14 | mouse | 67071 | Rps6ka6 | ribosomal protein S6 kinase... | NM_025949.3 | 75.5% | 76.1% | (many diffs) |
| 15 | mouse | 67071 | Rps6ka6 | ribosomal protein S6 kinase... | XM_006528400.3 | 75.5% | 76.1% | (many diffs) |
| 16 | mouse | 67071 | Rps6ka6 | ribosomal protein S6 kinase... | XM_006528401.3 | 75.5% | 76.1% | (many diffs) |
| 17 | mouse | 67071 | Rps6ka6 | ribosomal protein S6 kinase... | XM_006528402.2 | 75.1% | 75.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2307
- ORF length:
- 2235
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgctacca ttcgctcctc aggacgagcc ctgggaccga gaaatggaag 121 tgttcagcgg cggcggcgcg agcagcggcg aggtaaatgg tcttaaaatg gttgatgagc 181 caatggaaga gggagaagca gattcttgtc atgatgaagg agttgttaaa gaaatcccta 241 ttactcatca tgttaaggaa ggctatgaga aagcagatcc tgcacagttt gagttgctca 301 aggttcttgg tcaggggtca tttggaaagg tttttcttgt tagaaagaag accggtcctg 361 atgctgggca gctctatgca atgaaggtgt taaaaaaagc ctctttaaaa gttcgagaca 421 gagttcggac aaagatggag agggatatac tggtggaagt aaatcatcca tttattgtca 481 aattgcacta tgcctttcag actgaaggga aactgtactt aatactggat tttctcaggg 541 gaggagatgt tttcacaaga ttatccaaag aggttctgtt tacagaggaa gatgtgaaat 601 tctacctcgc agaactggcc cttgctttgg atcatctgca ccaattagga attgtttata 661 gagacctgaa gccagaaaac attttgcttg atgaaatagg acatatcaaa ttaacagatt 721 ttggactcag caaggagtca gtagatcaag aaaagaaggc ttactcattt tgtggtacag 781 tagagtatat ggctcctgaa gtagtaaata ggagaggcca ttcccagagt gctgattggt 841 ggtcatatgg tgttcttatg tttgaaatgc ttactggtac tctgccattt caaggtaaag 901 acagaaatga gaccatgaat atgatattaa aagcaaaact tggaatgcct caatttctta 961 gtgctgaagc acaaagtctt ctaaggatgt tattcaaaag gaatccagca aatagattgg 1021 gatcagaagg agttgaagaa atcaaaagac atctgttttt tgcaaatatt gactgggata 1081 aattatataa aagagaagtt caacctcctt tcaaacctgc ttctggaaaa ccagatgata 1141 ctttttgttt tgatcctgaa tttactgcaa aaacacctaa agattctccc ggtttgccag 1201 ccagtgcaaa tgctcatcag ctcttcaaag gattcagctt tgttgcaact tctattgcag 1261 aagaatataa aatcactcct atcacaagtg caaatgtatt accaattgtt cagataaatg 1321 gaaatgctgc acaatttggt gaagtatatg aattgaagga ggatattggt gttggctcct 1381 actctgtttg caagcgatgc atacatgcaa ctaccaacat ggaatttgca gtgaagatca 1441 ttgacaaaag taagcgagac ccttcagaag agattgaaat attgatgcgc tatggacaac 1501 atcccaacat tattactttg aaggatgtct ttgatgatgg tagatatgtt taccttgtta 1561 cggatttaat gaaaggagga gagttacttg accgtattct caaacaaaaa tgtttctcgg 1621 aacgggaggc tagtgatata ctatatgtaa taagtaagac agttgactat cttcattgtc 1681 aaggagttgt tcatcgtgat cttaaaccta gtaatatttt atacatggat gaatcagcca 1741 gtgcagattc aatcaggata tgtgattttg ggtttgcaaa acaacttcga ggagaaaatg 1801 gacttctctt aactccatgc tacactgcaa actttgttgc acctgaggtt cttatgcaac 1861 agggatatga tgctgcttgt gatatctgga gtttaggagt ccttttttac acaatgttGG 1921 CTGGCTACAC TCCATTTGCT AATGGCCCCA ATGATACTCC TGAAGAGATA CTGCTGCGTA 1981 TAGGCAATGG AAAATTCTCT TTGAGTGGTG GAAACTGGGA CAATATTTCA GACGGAGCAA 2041 AGGATTTGCT TTCCCATATG CTTCATATGG ACCCACATCA GCGGTATACT GCTGAACAAA 2101 TATTAAAGCA CTCATGGATA ACTCACAGAG ACCAGTTGCC AAATGATCAG CCAAAGAGAA 2161 ATGATGTGTC ACATGTTGTT AAGGGAGCAA TGGTTGCAAC ATACTCTGCC CTGACTCACA 2221 AGACCTTTCA ACCAGTCCTA GAGCCTGTAG CTGCTTCAAG CTTAGCCCAG CGACGGAGCA 2281 TGAAAAAGCG AACATCAACT GGCCTGTGAG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 2341 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2401 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAATTAG 2461 TCCATTGAAT GAACGATATA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2521 tgaaagatt