Construct: ORF TRCN0000489500
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019297.1_s317c1
- DNA Barcode:
- AAACCTTTTAACAAGGGCAAGTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RXFP2 (122042)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489500
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 122042 | RXFP2 | relaxin family peptide rece... | NM_130806.5 | 100% | 100% | |
| 2 | human | 122042 | RXFP2 | relaxin family peptide rece... | NM_001166058.1 | 96.8% | 96.8% | 855_856ins72 |
| 3 | human | 122042 | RXFP2 | relaxin family peptide rece... | XM_017020389.1 | 50.6% | 50.5% | 1146G>T;1148_1149delGCins1115 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 2337
- ORF length:
- 2262
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgatt gtttttctgg tttttaaaca tctcttcagc ctcagattga 121 ttacaatgtt ctttctactt catttcatcg ttctgatcaa tgtcaaagat tttgcactga 181 ctcaaggtag catgatcact ccttcatgcc aaaaaggata ttttccctgt gggaatctta 241 ccaagtgctt accccgagct tttcactgtg atggcaagga tgactgtggg aacggggcgg 301 acgaagagaa ctgtggtgac actagtggat gggcgaccat atttggcaca gtgcatggaa 361 atgctaacag cgtggcctta acacaggagt gctttctaaa acagtatcca caatgctgtg 421 actgcaaaga aactgaattg gaatgtgtaa atggtgactt aaagtctgtg ccgatgattt 481 ctaacaatgt gacattactg tctcttaaga aaaacaaaat ccacagtctt ccagataaag 541 ttttcatcaa atacacaaaa cttaaaaaga tatttcttca gcataattgc attagacaca 601 tatccaggaa agcatttttt ggattatgta atctgcaaat attatatctc aaccacaact 661 gcatcacaac cctcagacct ggaatattca aagacttaca tcagctaact tggctaattc 721 tagatgacaa tccaataacc agaatttcac agcgcttgtt tacgggatta aattccttgt 781 ttttcctgtc tatggttaat aactacttag aagctcttcc caagcagatg tgtgcccaaa 841 tgcctcaact caactgggtg gatttggaag gcaatagaat aaagtatctc acaaattcta 901 cgtttctgtc gtgcgattcg ctcacagtgc tgtttctgcc tagaaatcaa attggttttg 961 ttccagagaa gacattttct tcattaaaaa atttaggaga actggatctg tctagcaata 1021 cgataacgga actatcacct caccttttta aagacttgaa gcttctacaa aagctgaacc 1081 tgtcatccaa tcctcttatg tatcttcaca agaaccagtt tgaaagtctt aaacaacttc 1141 agtctctaga cctggaaagg atagagattc caaatataaa cacacgaatg tttcaaccca 1201 tgaagaatct ttctcacatt tatttcaaaa actttcgata ctgctcctat gctccccatg 1261 tccgaatatg tatgcccttg acggacggca tttcttcatt tgaggacctc ttggctaaca 1321 atatcctcag aatatttgtc tgggttatag ctttcattac ctgctttgga aatctttttg 1381 tcattggcat gagatctttc attaaagctg aaaatacaac tcacgctatg tccatcaaaa 1441 tcctttgttg tgctgattgc ctgatgggtg tttacttgtt ctttgttggc attttcgata 1501 taaaataccg agggcagtat cagaagtatg ccttgctgtg gatggagagc gtgcagtgcc 1561 gcctcatggg gttcctggcc atgctgtcca ccgaagtctc tgttctgcta ctgacctact 1621 tgactttgga gaagttcctg gtcattgtct tccccttcag taacattcga cctggaaaac 1681 ggcagacctc agtcatcctc atttgcatct ggatggcggg atttttaata gctgtaattc 1741 cattttggaa taaggattat tttggaaact tttatgggaa aaatggagta tgtttcccac 1801 tttattatga ccaaacagaa gatattggaa gcaaagggta ttctcttgga attttcctag 1861 gtgtgaactt gctggctttt ctcatcattg tgttttccta tattactatg ttctgttcca 1921 ttcaaaaaac cgccttgcag accacagaag taaggaattg ttttgGAAGA GAGGTGGCTG 1981 TTGCAAATCG TTTCTTTTTT ATAGTGTTCT CTGATGCCAT CTGCTGGATT CCTGTATTTG 2041 TAGTTAAAAT CCTTTCCCTC TTCCGGGTGG AAATACCAGA CACAATGACT TCCTGGATAG 2101 TGATTTTTTT CCTTCCAGTT AACAGTGCTT TGAATCCAAT CCTCTATACT CTCACAACCA 2161 ACTTTTTTAA GGACAAGTTG AAACAGCTGC TGCACAAACA TCAGAGGAAA TCAATTTTCA 2221 AAATTAAAAA AAAAAGTTTA TCTACATCCA TTGTGTGGAT AGAGGACTCC TCTTCCCTGA 2281 AACTTGGGGT TTTGAACAAA ATAACACTTG GAGACAGTAT AATGAAACCA GTTTCCGACC 2341 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2401 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2461 ATATATCTTG TGGAAAGGAC GAAAACCTTT TAACAAGGGC AAGTTTACGC GTTAAGTCga 2521 caatcaacct ctggattaca aaatttgtga aagatt