Construct: ORF TRCN0000489507
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019426.1_s317c1
- DNA Barcode:
- GATTCGATGTGGCGGGCTAATGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR143 (4935)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489507
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4935 | GPR143 | G protein-coupled receptor 143 | NM_000273.3 | 95.1% | 95.2% | 0_1ins60;1211_1212delTA |
| 2 | human | 4935 | GPR143 | G protein-coupled receptor 143 | XM_005274541.3 | 90.4% | 88% | (many diffs) |
| 3 | human | 4935 | GPR143 | G protein-coupled receptor 143 | XM_024452387.1 | 75.3% | 75.4% | 0_1ins312;959_960delTA |
| 4 | human | 4935 | GPR143 | G protein-coupled receptor 143 | XM_024452388.1 | 75.3% | 75.4% | 0_1ins312;959_960delTA |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1341
- ORF length:
- 1272
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gacccaggca ggccggcggg gtcctggcac acccgagccg cgtccgcgaa 121 cacagcccat ggcctccccg cgcctaggga ccttctgctg ccccacgcgg gacgcagcca 181 cgcagctcgt gctgagcttc cagccgcggg ccttccacgc gctctgcctg ggcagcggcg 241 ggctccgctt ggcgctgggc cttctgcagc tgctgcccgg ccgccggccc gcgggccccg 301 ggtcccccgc gacgtccccg ccggcctcgg tccgcatcct gcgcgctgcc gctgcctgcg 361 accttctcgg ctgcctgggt atggtgatcc ggtccaccgt gtggttagga ttcccaaatt 421 ttgttgacag cgtctcggat atgaaccaca cggaaatttg gcctgctgct ttctgcgtgg 481 ggagtgcgat gtggatccag ctgttgtaca gtgcctgctt ctggtggctg ttttgctatg 541 cagtggatgc ttatctggtg atccggagat cggcaggact gagcaccatc ctgctgtatc 601 acatcatggc gtggggcctg gccaccctgc tctgtgtgga gggagccgcc atgctctact 661 acccttccgt gtccaggtgt gagcggggcc tggaccacgc catcccccac tatgtcacca 721 tgtacctgcc cctgctgctg gttctcgtgg cgaaccccat cctgttccaa aagacagtga 781 ctgcagtggc ctctttactt aaaggaagac aaggcattta cacggagaac gagaggagga 841 tgggagccgt gatcaagatc cgatttttca aaatcatgct ggttttaatt atttgttggt 901 tgtcgaatat catcaatgaa agccttttat tctatcttga gatgcaaaca gatatcaatg 961 gaggttcttt gaaaCCTGTC AGAACTGCAG CCAAGACCAC ATGGTTTATT ATGGGAATCC 1021 TGAATCCAGC CCAGGGATTT CTCTTGTCTT TGGCCTTCTA CGGCTGGACA GGATGCAGCC 1081 TGGGTTTTCA GTCTCCCAGG AAGGAGATCC AGTGGGAATC ACTGACCACC TCGGCTGCTG 1141 AGGGGGCTCA CCCATCCCCA CTGATGCCCC ATGAAAACCC TGCTTCCGGG AAGGTGTCTC 1201 AAGTGGGTGG GCAGACTTCT GACGAAGCCC TGAGCATGCT GTCTGAAGGT TCTGATGCCA 1261 GCACAATTGA AATTCACACT GCAAGTGAAT CCTGCAACAA AAATGAGGGT GACCCTGCTC 1321 TCCCAACCCA TGGAGACCTA AACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1381 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1441 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGATT CGATGTGGCG 1501 GGCTAATGAC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt