Construct: ORF TRCN0000489516
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021287.1_s317c1
- DNA Barcode:
- GCGGCCGACCCGTCATACTAATAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TMEM177 (80775)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489516
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80775 | TMEM177 | transmembrane protein 177 | NM_001105198.1 | 99.7% | 99.3% | 94A>G;801C>G |
| 2 | human | 80775 | TMEM177 | transmembrane protein 177 | NM_001105199.2 | 99.6% | 99.3% | 94A>G;267A>G;801C>G |
| 3 | human | 80775 | TMEM177 | transmembrane protein 177 | NM_030577.3 | 99.6% | 99.3% | 94A>G;267A>G;801C>G |
| 4 | human | 80775 | TMEM177 | transmembrane protein 177 | XR_002959349.1 | 41.6% | (many diffs) | |
| 5 | human | 80775 | TMEM177 | transmembrane protein 177 | XR_002959347.1 | 38% | (many diffs) | |
| 6 | human | 80775 | TMEM177 | transmembrane protein 177 | XR_002959346.1 | 37.7% | (many diffs) | |
| 7 | human | 80775 | TMEM177 | transmembrane protein 177 | XR_002959348.1 | 33.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1005
- ORF length:
- 933
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcaggt cccctgtggc ggaccgcagc atttgtgcag agacacagga 121 caggcctctt ggtgggttcc tgtgcaggcc tgtttggagt tccagtctcg taccacctct 181 tcccggatcc cgtggtccaa tggctctacc agtactggcc tcagggccag ccagctccgc 241 tccctccaca gctgcagagc ctcttccaag aggtgctaca ggacataggt gttccttcag 301 gccattgcta caagcccttc accaccttca ccttccagcc tgtgagtgca ggcttcccaa 361 gactccctgc tggggctgtg gtgggcatcc ctgccagttt cttgggagac ctagtgatca 421 acactaacca tcccgtggtc atacatgggc atacagtgga ctggcggagc ccagcaggcg 481 cccggctgag agcttccctg accttgtccc gtgaagccca gaagttcgcc ttggccaggg 541 aagtggtgta cctggaaagc agtaccactg ccgtgcacgc cctgctGGCC CCAGCTTGCC 601 TGGCAGGGAC CTGGGCACTG GGCGTGGGTG CCAAGTACAC CCTGGGGCTC CATGCAGGCC 661 CCATGAATTT ACGGGCTGCC TTCAGCTTGG TGGCAGCAGT GGCAGGCTTT GTGGCCTACG 721 CCTTCTCCCA GGATTCTCTC ACTCATGCCG TGGAGTCCTG GCTGGACCGC CGCACGGCCT 781 CCCTCTCTGC AGCCTATGCC TGTGGTGGAG TGGAGTTCTA TGAGAAGCTT CTGTCGGGCA 841 ACCTGGCCCT GCGCAGTCTC TTGGGCAAAG AGGGGGAGAA GCTGTATACA CCCAGCGGGA 901 ACATCGTCCC CAGACACTTG TTCCGAATCA AACATTTACC CTACACCACC CGCCGGGACT 961 CTGTGCTGCA GATGTGGAGG GGGATGCTCA ATCCGGGCCG CTCCTAGAAC CCAGCTTTCT 1021 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1081 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1141 GTGGAAAGGA CGAGCGGCCG ACCCGTCATA CTAATAAACG CGTTAAGTCg acaatcaacc 1201 tctggattac aaaatttgtg aaagatt