Construct: ORF TRCN0000489520
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021144.1_s317c1
- DNA Barcode:
- TATAGCGGGATGGACCCCGAGCCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TAS2R43 (259289)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489520
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 259289 | TAS2R43 | taste 2 receptor member 43 | NM_176884.2 | 99.6% | 99.3% | 104G>C;635A>G;663C>G |
| 2 | human | 259290 | TAS2R31 | taste 2 receptor member 31 | NM_176885.2 | 94.3% | 88.9% | (many diffs) |
| 3 | human | 259292 | TAS2R46 | taste 2 receptor member 46 | NM_176887.2 | 93% | 87.3% | (many diffs) |
| 4 | human | 259291 | TAS2R45 | taste 2 receptor member 45 | NM_176886.2 | 89.3% | 82.5% | (many diffs) |
| 5 | human | 259289 | TAS2R43 | taste 2 receptor member 43 | XM_003960991.5 | 89.3% | 82.5% | (many diffs) |
| 6 | human | 259293 | TAS2R30 | taste 2 receptor member 30 | NM_001097643.1 | 88.6% | 77.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 999
- ORF length:
- 927
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgataact tttctaccca tcattttttc cagtctggta gtggttacat 121 ttgttattgg aaattttgct aatggcttca tagcactggt aaattccatt gagtcgttca 181 agagacaaaa gatctccttt gctgaccaaa ttctcactgc tctggcggtc tccagagttg 241 gtttgctctg ggtattatta ttaaactggt attcaactgt gttgaatcca gcttttaata 301 gtgtagaagt aagaactact gcttataata tctgggcagt gatcaaccat ttcagcaact 361 ggcttgctac taccctcagc atattttatt tgctcaagat tgccaatttc tccaacttta 421 tttttcttca cttaaagagg agagttaaga gtgtcattct ggtgatgttg ttggggcctt 481 tgctattttt ggcttgtcat ctttttgtga taaacatgaa tgagattgtg cggacaaaag 541 aatttgaagg aaacatgact tggaagatca aattgaagag tgcaatgtac ttttcaaata 601 tgactgtaac catggtagca aacttagtac ccttcactct gacccTACTA TCTTTTATGC 661 TGTTAATCTG TTCTTTGTGT AAACATCTCA AGAAGATGCA GCTCCGTGGT AAAGGATCTC 721 AAGATCCCAG CACGAAGGTC CACATAAAAG CTTTGCAAAC TGTGATCTCC TTCCTCTTGT 781 TATGTGCCAT TTACTTTCTG TCCATAATGA TATCAGTTTG GAGTTTTGGA AGTCTGGAAA 841 ACAAACCTGT CTTCATGTTC TGCAAAGCTA TTAGATTCAG CTATCCTTCA ATCCACCCAT 901 TCATCCTGAT TTGGGGAAAC AAGAAGCTAA AGCAGACTTT TCTTTCAGTT TTTTGGCAAA 961 TGAGGTACTG GGTGAAAGGA GAGAAGACTT CATCTCCATA GAACCCAGCT TTCTTGTACA 1021 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1081 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1141 AGGACGATAT AGCGGGATGG ACCCCGAGCC CACGCGTTAA GTCgacaatc aacctctgga 1201 ttacaaaatt tgtgaaagat t