Construct: ORF TRCN0000489534
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019782.1_s317c1
- DNA Barcode:
- AGCCACTTTTCAATCACTGCGGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DAP (1611)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489534
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1611 | DAP | death associated protein | NM_004394.3 | 99.6% | 99% | 306_307insG |
| 2 | human | 1611 | DAP | death associated protein | NM_001291963.2 | 40.1% | 26.4% | 151_152ins43;264_612delinsG |
| 3 | mouse | 223453 | Dap | death-associated protein | NM_146057.3 | 86.3% | 95.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 381
- ORF length:
- 309
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtcttcg cctcccgaag ggaaactaga gactaaagct ggacacccgc 121 ccgccgtgaa agctggtgga atgcgaattg tGCAGAAACA CCCACATACA GGAGACACCA 181 AAGAAGAGAA AGACAAGGAT GACCAGGAAT GGGAAAGCCC CAGTCCACCT AAACCCACTG 241 TGTTCATCTC TGGGGTCATC GCCCGGGGTG ACAAAGATTT CCCCCCGGCG GCTGCGCAGG 301 TGGCTCACCA GAAGCCGCAT GCCTCCATGG ACAAGCATCC TTCCCCAAGA ACCCAGCACA 361 TCCAGCAGCC ACGCAAGGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 421 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTTGAAAG TATTTCGATT 481 TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAGCCACTT TTCAATCACT GCGGCGACGC 541 GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt