Construct: ORF TRCN0000489573
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020761.1_s317c1
- DNA Barcode:
- CACCCCACTTGGCTGGGTCACCAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CYSLTR2 (57105)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489573
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_001308465.3 | 95.3% | 95.3% | 1_48del |
| 2 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_001308467.3 | 95.3% | 95.3% | 1_48del |
| 3 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_001308468.3 | 95.3% | 95.3% | 1_48del |
| 4 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_001308469.3 | 95.3% | 95.3% | 1_48del |
| 5 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_001308470.3 | 95.3% | 95.3% | 1_48del |
| 6 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_001308471.3 | 95.3% | 95.3% | 1_48del |
| 7 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_001308476.3 | 95.3% | 95.3% | 1_48del |
| 8 | human | 57105 | CYSLTR2 | cysteinyl leukotriene recep... | NM_020377.5 | 95.3% | 95.3% | 1_48del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1062
- ORF length:
- 990
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaacca aatggcacct tcagcaataa caacagcagg aactgcacaa 121 ttgaaaactt caagagagaa tttttcccaa ttgtatatct gataatattt ttctggggag 181 tcttgggaaa tgggttgtcc atatatgttt tcctgcagcc ttataagaag tccacatctg 241 tgaacgtttt catgctaaat ctggccattt cagatctcct gttcataagc acgcttccct 301 tcagggctga ctattatctt agaggctcca attggatatt tggagacctg gcctgcagga 361 ttatgtctta ttccttgtat gtcaacatgt acagcagtat ttatttcctg accgtgctga 421 gtgttgtgcg tttcctggca atggttcacc cctttcggct tctgcatgtc accagcatca 481 ggagtgcctg gatcctctgt gggatcatat ggatccttat catggcttcc tcaataatgc 541 tcctggacag tggctctgag cagaacggca gtgtcacatc atgcttagag ctgaatctct 601 ataaaattgc taagctgcag accatgaact atattgcctt GGTGGTGGGC TGCCTGCTGC 661 CATTTTTCAC ACTCAGCATC TGTTATCTGC TGATCATTCG GGTTCTGTTA AAAGTGGAGG 721 TCCCAGAATC GGGGCTGCGG GTTTCTCACA GGAAGGCACT GACCACCATC ATCATCACCT 781 TGATCATCTT CTTCTTGTGT TTCCTGCCCT ATCACACACT GAGGACCGTC CACTTGACGA 841 CATGGAAAGT GGGTTTATGC AAAGACAGAC TGCATAAAGC TTTGGTTATC ACACTGGCCT 901 TGGCAGCAGC CAATGCCTGC TTCAATCCTC TGCTCTATTA CTTTGCTGGG GAGAATTTTA 961 AGGACAGACT AAAGTCTGCA CTCAGAAAAG GCCATCCACA GAAGGCAAAG ACAAAGTGTG 1021 TTTTCCCTGT TAGTGTGTGG TTGAGAAAGG AAACAAGAGT ATAGGACCCA GCTTTCTTGT 1081 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1141 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1201 GAAAGGACGA CACCCCACTT GGCTGGGTCA CCATACGCGT TAAGTCgaca atcaacctct 1261 ggattacaaa atttgtgaaa gatt