Construct: ORF TRCN0000489582
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020082.1_s317c1
- DNA Barcode:
- CATGTCCGGCCTTAAAGATGAAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BUB1 (699)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489582
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 699 | BUB1 | BUB1 mitotic checkpoint ser... | NM_004336.5 | 99.9% | 99.9% | 3255_3256insG |
| 2 | human | 699 | BUB1 | BUB1 mitotic checkpoint ser... | NM_001278616.1 | 98.1% | 98% | 26_27ins60;3195_3196insG |
| 3 | human | 699 | BUB1 | BUB1 mitotic checkpoint ser... | NM_001278617.1 | 94.7% | 94.6% | 2625_2626ins171;3084_3085insG |
| 4 | human | 699 | BUB1 | BUB1 mitotic checkpoint ser... | XR_923001.3 | 93% | 1_68del;2530_2570del;3365_3499delinsG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3327
- ORF length:
- 3258
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggacaccccg gaaaatgtcc ttcagatgct tgaagcccac atgcagagct 121 acaagggcaa tgaccctctt ggtgaatggg aaagatacat acagtgggta gaagagaatt 181 ttcctgagaa taaagaatac ttgataactt tactagaaca tttaatgaag gaatttttag 241 ataagaagaa ataccacaat gacccaagat tcatcagtta ttgtttaaaa tttgctgagt 301 acaacagtga cctccatcaa ttttttgagt ttctgtacaa ccatgggatt ggaaccctgt 361 catcccctct gtacattgcc tgggcggggc atctggaagc ccaaggagag ctgcagcatg 421 ccagtgctgt ccttcagaga ggaattcaaa accaggctga acccagagag ttcctgcaac 481 aacaatacag gttatttcag acacgcctca ctgaaaccca tttgccagct caagctagaa 541 cctcagaacc tctgcataat gttcaggttt taaatcaaat gataacatca aaatcaaatc 601 caggaaataa catggcctgc atttctaaga atcagggttc agagctttct ggagtgatat 661 cttcagcttg tgataaagag tcaaatatgg aacgaagagt gatcacgatt tctaaatcag 721 aatattctgt gcactcatct ttggcatcca aagttgatgt tgagcaggtt gttatgtatt 781 gcaaggagaa gcttattcgt ggggaatcag aattttcctt tgaagaattg agagcccaga 841 aatacaatca acggagaaag catgagcaat gggtaaatga agacagacat tatatgaaaa 901 ggaaagaagc aaatgctttt gaagaacagc tattaaaaca gaaaatggat gaacttcata 961 agaagttgca tcaggtggtg gagacatccc atgaggatct gcccgcttcc caggaaaggt 1021 ccgaggttaa tccagcacgt atggggccaa gtgtaggctc ccagcaggaa ctgagagcgc 1081 catgtcttcc agtaacctat cagcagacac cagtgaacat ggaaaagaac ccaagagagg 1141 cacctcctgt tgttcctcct ttggcaaatg ctatttctgc agctttggtg tccccagcca 1201 ccagccagag cattgctcct cctgttcctt tgaaagccca gacagtaaca gactccatgt 1261 ttgcagtggc cagcaaagat gctggatgtg tgaataagag tactcatgaa ttcaagccac 1321 agagtggagc agagatcaaa gaagggtgtg aaacacataa ggttgccaac acaagttctt 1381 ttcacacaac tccaaacaca tcactgggaa tggttcaggc aacgccatcc aaagtgcagc 1441 catcacccac cgtgcacaca aaagaagcat taggtttcat catgaatatg tttcaggctc 1501 ctacacttcc tgatatttct gatgacaaag atgaatggca atctctagat caaaatgaag 1561 atgcatttga agcccagttt caaaaaaatg taaggtcatc tggggcttgg ggagtcaata 1621 agatcatctc ttctttgtca tctgcttttc atgtgtttga agatggaaac aaagaaaatt 1681 atggattacc acagcctaaa aataaaccca caggagccag gacctttgga gaacgctctg 1741 tcagcagact tccttcaaaa ccaaaggagg aagtgcctca tgctgaagag tttttggatg 1801 actcaactgt atggggtatt cgctgcaaca aaaccctggc acccagtcct aagagcccag 1861 gagacttcac atctgctgca caacttgcgt ctacaccatt ccacaagctt ccagtggagt 1921 cagtgcacat tttagaagat aaagaaaatg tggtagcaaa acagtgtacc caggcgactt 1981 tggattcttg tgaggaaaac atggtggtgc cttcaaggga tggaaaattc agtccaattc 2041 aagagaaaag cccaaaacag gccttgtcgt ctcacatgta ttcagcatcc ttacttcgtc 2101 tgagccagcc tgctgcaggt ggggtactta cctgtgaggc agagttgggc gttgaggctt 2161 gcagactcac agacactgac gctgccattg cagaagatcc accagatgct attgctgggc 2221 tccaagcaga atggatgcag atgagttcac ttgggactgt tgatgctcca aacttcattg 2281 ttgggaaccc atgggatgat aagctgattt tcaaactttt atctgggctt tctaaaccag 2341 tgagttccta tccaaatact tttgaatggc aatgtaaact tccagccatc aagcccaaga 2401 ctgaatttca attgggttct aagctggtct atgtccatca ccttcttgga gaaggagcct 2461 ttgcccaggt gtacgaagct acccagggag atctgaatga tgctaaaaat aaacagaaat 2521 ttgttttaaa ggtccaaaag cctgccaacc cctgggaatt ctacattggg acccagttga 2581 tggaaagact aaagccatct atgcagcaca tgtttatgaa gttctattct gcccacttat 2641 tccagaatgg cagtgtatta gtaggagagc tctacagcta tggaacatta ttaaatgcca 2701 ttaacctcta taaaaatacc cctgaaaaag tgatgcctca aggtcttgtc atctcttttg 2761 ctatgagaat gctttacatg attgagcaag tgcatgactg tgaaatcatt catggagaca 2821 ttaaaccaga caatttcata cttggaaacg gatttttgga acaggatgat gaagatgatt 2881 tatctgctgg cttggcactg attgacctGG GTCAGAGTAT AGATATGAAA CTTTTTCCAA 2941 AAGGAACTAT ATTCACAGCA AAGTGTGAAA CATCTGGTTT TCAGTGTGTT GAGATGCTCA 3001 GCAACAAACC ATGGAACTAC CAGATCGATT ACTTTGGGGT TGCTGCAACA GTATATTGCA 3061 TGCTCTTTGG CACTTACATG AAAGTGAAAA ATGAAGGAGG AGAGTGTAAG CCTGAAGGTC 3121 TTTTTAGAAG GCTTCCTCAT TTGGATATGT GGAATGAATT TTTTCATGTT ATGTTGAATA 3181 TTCCAGATTG TCATCATCTT CCATCTTTGG ATTTGTTAAG GCAAAAGCTG AAGAAAGTAT 3241 TTCAACAACA CTATACTAAC AAGATTAGGG CCCTACGTAA TAGGCTAATT GTACTGCTCT 3301 TAGAATGTAA GCGTTCACGA AAAGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 3361 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 3421 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC ATGTCCGGCC 3481 TTAAAGATGA AAAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 3541 att