Construct: ORF TRCN0000489585
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019906.1_s317c1
- DNA Barcode:
- CGTAACTCAGACGTCTCGAGATGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRK2 (156)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489585
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 156 | GRK2 | G protein-coupled receptor ... | NM_001619.5 | 99.9% | 99.8% | 96C>A;2067_2068insG |
| 2 | human | 156 | GRK2 | G protein-coupled receptor ... | XM_024448376.1 | 95.8% | 93.6% | (many diffs) |
| 3 | human | 156 | GRK2 | G protein-coupled receptor ... | XM_011544773.1 | 95.2% | 94.7% | (many diffs) |
| 4 | human | 156 | GRK2 | G protein-coupled receptor ... | XR_001747779.1 | 57.8% | (many diffs) | |
| 5 | mouse | 110355 | Grk2 | G protein-coupled receptor ... | NM_001290818.1 | 90.1% | 98.6% | (many diffs) |
| 6 | mouse | 110355 | Grk2 | G protein-coupled receptor ... | NM_130863.2 | 84.3% | 92.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2139
- ORF length:
- 2070
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggcggacctg gaggcggtgc tggccgacgt gagctacctg atggccatgg 121 agaagagcaa ggccacgccg gccgcgcgcg ccagcaagaa gatactgctg cccgagccca 181 gcatccgcag tgtcatgcag aagtacctgg aggaccgggg cgaggtgacc tttgagaaga 241 tcttttccca gaagctgggg tacctgctct tccgagactt ctgcctgaac cacctggagg 301 aggccaggcc cttggtggaa ttctatgagg agatcaagaa gtacgagaag ctggagacgg 361 aggaggagcg tgtggcccgc agccgggaga tcttcgactc atacatcatg aaggagctgc 421 tggcctgctc gcatcccttc tcgaagagtg ccactgagca tgtccaaggc cacctgggga 481 agaagcaggt gcctccggat ctcttccagc catacatcga agagatttgt caaaacctcc 541 gaggggacgt gttccagaaa ttcattgaga gcgataagtt cacacggttt tgccagtgga 601 agaatgtgga gctcaacatc cacctgacca tgaatgactt cagcgtgcat cgcatcattg 661 ggcgcggggg ctttggcgag gtctatgggt gccggaaggc tgacacaggc aagatgtacg 721 ccatgaagtg cctggacaaa aagcgcatca agatgaagca gggggagacc ctggccctga 781 acgagcgcat catgctctcg ctcgtcagca ctggggactg cccattcatt gtctgcatgt 841 catacgcgtt ccacacgcca gacaagctca gcttcatcct ggacctcatg aacggtgggg 901 acctgcacta ccacctctcc cagcacgggg tcttctcaga ggctgacatg cgcttctatg 961 cggccgagat catcctgggc ctggagcaca tgcacaaccg cttcgtggtc taccgggacc 1021 tgaagccagc caacatcctt ctggacgagc atggccacgt gcggatctcg gacctgggcc 1081 tggcctgtga cttctccaag aagaagcccc atgccagcgt gggcacccac gggtacatgg 1141 ctccggaggt cctgcagaag ggcgtggcct acgacagcag tgccgactgg ttctctctgg 1201 ggtgcatgct cttcaagttg ctgcgggggc acagcccctt ccggcagcac aagaccaaag 1261 acaagcatga gatcgaccgc atgacgctga cgatggccgt ggagctgccc gactccttct 1321 cccctgaact acgctccctg ctggaggggt tgctgcagag ggatgtcaac cggagattgg 1381 gctgcctggg ccgaggggct caggaggtga aagagagccc ctttttccgc tccctggact 1441 ggcagatggt cttcttgcag aagtaccctc ccccgctgat ccccccacga ggggaggtga 1501 acgcggccga cgccttcgac attggctcct tcgatgagga ggacacaaaa ggaatcaagt 1561 tactggacag tgatcaggag ctctaccgca acttccccct caccatctcg gagcggtggc 1621 agcaggaggt ggcagagact gtcttcgaca ccatcaacgc tgagacagac cggctggagg 1681 ctcgcaagaa agccaagaac aagcagctgg gccatgagga agactacgcc ctgggcaagg 1741 actgcatcat gcatggctac atgtccaaga tgggcaaccc cttccTGACC CAGTGGCAGC 1801 GGCGGTACTT CTACCTGTTC CCCAACCGCC TCGAGTGGCG GGGCGAGGGC GAGGCCCCGC 1861 AGAGCCTGCT GACCATGGAG GAGATCCAGT CGGTGGAGGA GACGCAGATC AAGGAGCGCA 1921 AGTGCCTGCT CCTCAAGATC CGCGGTGGGA AACAGTTCAT TTTGCAGTGC GATAGCGACC 1981 CTGAGCTGGT GCAGTGGAAG AAGGAGCTGC GCGACGCCTA CCGCGAGGCC CAGCAGCTGG 2041 TGCAGCGGGT GCCCAAGATG AAGAACAAGC CGCGCTCGCC CGTGGTGGAG CTGAGCAAGG 2101 TGCCGCTGGT CCAGCGCGGC AGTGCCAACG GCCTCGACCC AGCTTTCTTG TACAAAGTGG 2161 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2221 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2281 ACGTAACTCA GACGTCTCGA GATGAACGCG TTAAGTCgac aatcaacctc tggattacaa 2341 aatttgtgaa agatt