Construct: ORF TRCN0000489585

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019906.1_s317c1
DNA Barcode:
CGTAACTCAGACGTCTCGAGATGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GRK2 (156)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489585
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 156 GRK2 G protein-coupled receptor ... NM_001619.5 99.9% 99.8% 96C>A;2067_2068insG
2 human 156 GRK2 G protein-coupled receptor ... XM_024448376.1 95.8% 93.6% (many diffs)
3 human 156 GRK2 G protein-coupled receptor ... XM_011544773.1 95.2% 94.7% (many diffs)
4 human 156 GRK2 G protein-coupled receptor ... XR_001747779.1 57.8% (many diffs)
5 mouse 110355 Grk2 G protein-coupled receptor ... NM_001290818.1 90.1% 98.6% (many diffs)
6 mouse 110355 Grk2 G protein-coupled receptor ... NM_130863.2 84.3% 92.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2139
ORF length:
2070
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat ggcggacctg gaggcggtgc tggccgacgt gagctacctg atggccatgg
121agaagagcaa ggccacgccg gccgcgcgcg ccagcaagaa gatactgctg cccgagccca
181gcatccgcag tgtcatgcag aagtacctgg aggaccgggg cgaggtgacc tttgagaaga
241tcttttccca gaagctgggg tacctgctct tccgagactt ctgcctgaac cacctggagg
301aggccaggcc cttggtggaa ttctatgagg agatcaagaa gtacgagaag ctggagacgg
361aggaggagcg tgtggcccgc agccgggaga tcttcgactc atacatcatg aaggagctgc
421tggcctgctc gcatcccttc tcgaagagtg ccactgagca tgtccaaggc cacctgggga
481agaagcaggt gcctccggat ctcttccagc catacatcga agagatttgt caaaacctcc
541gaggggacgt gttccagaaa ttcattgaga gcgataagtt cacacggttt tgccagtgga
601agaatgtgga gctcaacatc cacctgacca tgaatgactt cagcgtgcat cgcatcattg
661ggcgcggggg ctttggcgag gtctatgggt gccggaaggc tgacacaggc aagatgtacg
721ccatgaagtg cctggacaaa aagcgcatca agatgaagca gggggagacc ctggccctga
781acgagcgcat catgctctcg ctcgtcagca ctggggactg cccattcatt gtctgcatgt
841catacgcgtt ccacacgcca gacaagctca gcttcatcct ggacctcatg aacggtgggg
901acctgcacta ccacctctcc cagcacgggg tcttctcaga ggctgacatg cgcttctatg
961cggccgagat catcctgggc ctggagcaca tgcacaaccg cttcgtggtc taccgggacc
1021tgaagccagc caacatcctt ctggacgagc atggccacgt gcggatctcg gacctgggcc
1081tggcctgtga cttctccaag aagaagcccc atgccagcgt gggcacccac gggtacatgg
1141ctccggaggt cctgcagaag ggcgtggcct acgacagcag tgccgactgg ttctctctgg
1201ggtgcatgct cttcaagttg ctgcgggggc acagcccctt ccggcagcac aagaccaaag
1261acaagcatga gatcgaccgc atgacgctga cgatggccgt ggagctgccc gactccttct
1321cccctgaact acgctccctg ctggaggggt tgctgcagag ggatgtcaac cggagattgg
1381gctgcctggg ccgaggggct caggaggtga aagagagccc ctttttccgc tccctggact
1441ggcagatggt cttcttgcag aagtaccctc ccccgctgat ccccccacga ggggaggtga
1501acgcggccga cgccttcgac attggctcct tcgatgagga ggacacaaaa ggaatcaagt
1561tactggacag tgatcaggag ctctaccgca acttccccct caccatctcg gagcggtggc
1621agcaggaggt ggcagagact gtcttcgaca ccatcaacgc tgagacagac cggctggagg
1681ctcgcaagaa agccaagaac aagcagctgg gccatgagga agactacgcc ctgggcaagg
1741actgcatcat gcatggctac atgtccaaga tgggcaaccc cttccTGACC CAGTGGCAGC
1801GGCGGTACTT CTACCTGTTC CCCAACCGCC TCGAGTGGCG GGGCGAGGGC GAGGCCCCGC
1861AGAGCCTGCT GACCATGGAG GAGATCCAGT CGGTGGAGGA GACGCAGATC AAGGAGCGCA
1921AGTGCCTGCT CCTCAAGATC CGCGGTGGGA AACAGTTCAT TTTGCAGTGC GATAGCGACC
1981CTGAGCTGGT GCAGTGGAAG AAGGAGCTGC GCGACGCCTA CCGCGAGGCC CAGCAGCTGG
2041TGCAGCGGGT GCCCAAGATG AAGAACAAGC CGCGCTCGCC CGTGGTGGAG CTGAGCAAGG
2101TGCCGCTGGT CCAGCGCGGC AGTGCCAACG GCCTCGACCC AGCTTTCTTG TACAAAGTGG
2161TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
2221CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
2281ACGTAACTCA GACGTCTCGA GATGAACGCG TTAAGTCgac aatcaacctc tggattacaa
2341aatttgtgaa agatt

Download FASTA (ORF) (Full)