Construct: ORF TRCN0000489591
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021905.1_s317c1
- DNA Barcode:
- AACTCCGGTTCCAACATGTTCTTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- POP5 (51367)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489591
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51367 | POP5 | POP5 homolog, ribonuclease ... | NM_015918.4 | 99.7% | 100% | 442T>C |
| 2 | human | 51367 | POP5 | POP5 homolog, ribonuclease ... | XM_011538441.1 | 89.8% | 90% | 312_365del;496T>C |
| 3 | human | 51367 | POP5 | POP5 homolog, ribonuclease ... | NM_198202.1 | 69.1% | 69.3% | 162_163ins150;292T>C |
| 4 | mouse | 117109 | Pop5 | processing of precursor 5, ... | NM_026398.4 | 82.6% | 86.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 558
- ORF length:
- 489
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggtgcggttc aagcacaggt acctgctctg cgaactggtg tctgacgacc 121 cccgctgccg cctaagcctc gatgaccgag ttctgagcag cctcgtacgg gacacgatcg 181 ccagggtgca cggaactttc ggcgcagccg ccTGCTCCAT CGGCTTCGCG GTTCGATATC 241 TCAATGCCTA TACTGGAATA GTGCTACTTC GATGCAGAAA AGAATTCTAT CAGCTTGTGT 301 GGTCAGCTCT TCCCTTCATC ACATACTTGG AGAACAAAGG ACACCGTTAC CCATGCTTTT 361 TCAACACATT ACATGTGGGA GGTACAATAA GAACATGTCA GAAGTTCCTA ATTCAGTACA 421 ACAGGAGACA GCTGTTGATC TTGTTGCAGA ACTGCACTGA TGAAGGAGAG CGGGAAGCTA 481 TCCAGAAGTC TGTGACAAGA AGCTGCTTAC TAGAGGAGGA GGAGGAGTCA GGTGAGGAGG 541 CTGCAGAAGC AATGGAGTAG AACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 601 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 661 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAACT CCGGTTCCAA 721 CATGTTCTTA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt