Construct: ORF TRCN0000489596
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021667.1_s317c1
- DNA Barcode:
- CGTATTCTTTATGGATGTCTAACC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PTK2B (2185)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489596
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2185 | PTK2B | protein tyrosine kinase 2 beta | NM_004103.4 | 99.8% | 99.9% | (many diffs) |
| 2 | human | 2185 | PTK2B | protein tyrosine kinase 2 beta | NM_173174.3 | 99.8% | 99.9% | (many diffs) |
| 3 | human | 2185 | PTK2B | protein tyrosine kinase 2 beta | NM_173176.3 | 99.8% | 99.9% | (many diffs) |
| 4 | human | 2185 | PTK2B | protein tyrosine kinase 2 beta | XM_005273447.4 | 99.8% | 99.9% | (many diffs) |
| 5 | human | 2185 | PTK2B | protein tyrosine kinase 2 beta | XM_011544441.2 | 99.8% | 99.9% | (many diffs) |
| 6 | human | 2185 | PTK2B | protein tyrosine kinase 2 beta | XM_017013214.1 | 99.8% | 99.9% | (many diffs) |
| 7 | human | 2185 | PTK2B | protein tyrosine kinase 2 beta | XM_005273448.5 | 98.6% | 98.7% | (many diffs) |
| 8 | human | 2185 | PTK2B | protein tyrosine kinase 2 beta | XM_011544442.3 | 98.5% | 98.6% | (many diffs) |
| 9 | human | 2185 | PTK2B | protein tyrosine kinase 2 beta | XM_017013215.1 | 98.5% | 98.6% | (many diffs) |
| 10 | human | 2185 | PTK2B | protein tyrosine kinase 2 beta | XM_017013216.2 | 97.3% | 97.4% | (many diffs) |
| 11 | human | 2185 | PTK2B | protein tyrosine kinase 2 beta | NM_173175.2 | 95.6% | 95.7% | (many diffs) |
| 12 | mouse | 19229 | Ptk2b | PTK2 protein tyrosine kinas... | NM_001162366.1 | 89.2% | 95.3% | (many diffs) |
| 13 | mouse | 19229 | Ptk2b | PTK2 protein tyrosine kinas... | XM_006518726.3 | 89.2% | 95.3% | (many diffs) |
| 14 | mouse | 19229 | Ptk2b | PTK2 protein tyrosine kinas... | XM_011244994.2 | 89.2% | 95.3% | (many diffs) |
| 15 | mouse | 19229 | Ptk2b | PTK2 protein tyrosine kinas... | NM_001162365.1 | 88.9% | 94.9% | (many diffs) |
| 16 | mouse | 19229 | Ptk2b | PTK2 protein tyrosine kinas... | XM_017315926.1 | 88.3% | 94.3% | (many diffs) |
| 17 | mouse | 19229 | Ptk2b | PTK2 protein tyrosine kinas... | XM_017315927.1 | 88.3% | 94.3% | (many diffs) |
| 18 | mouse | 19229 | Ptk2b | PTK2 protein tyrosine kinas... | XM_017315928.1 | 88.3% | 94.3% | (many diffs) |
| 19 | mouse | 19229 | Ptk2b | PTK2 protein tyrosine kinas... | XM_017315929.1 | 88.3% | 94.3% | (many diffs) |
| 20 | mouse | 19229 | Ptk2b | PTK2 protein tyrosine kinas... | NM_172498.3 | 85.3% | 91.1% | (many diffs) |
| 21 | mouse | 19229 | Ptk2b | PTK2 protein tyrosine kinas... | XM_017315930.1 | 84.4% | 90.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3096
- ORF length:
- 3027
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gtctggggtg tccgagcccc tgagtcgagt aaagttgggc acgttacgcc 121 ggcctgaagg ccctgcagag cccatggtgg tggtaccagt agatgtggaa aaggaggacg 181 tgcgtatcct caaggtctgc ttctatagca acagcttcaa tcctgggaaa aacttcaaac 241 tggtcaaatg cactgtccag acggagatcc gggagatcat cacctccatc ctgctgagcg 301 ggcggatcgg gcccaacatc cggttggctg agtgctatgg gctgaggctg aagcacatga 361 agtccgatga gatccactgg ctgcacccac agatgacagt gggtgaggtg caggacaagt 421 atgagtgtct gcacgtggaa gccgagtgga ggtatgacct tcaaatccgc tacttgccag 481 aagacttcat ggagagcctg aaggaggaca ggaccacgct gctctatttt taccaacagc 541 tccggaacga ctacatgcag cgctacgcca gcaaggtcag cgagggcatg gccctgcagc 601 tgggctgcct ggagctcagg cggttcttca aggatatgcc ccacaatgca cttgacaaga 661 agtccaactt cgagctccta gaaaaggaag tggggctgga cttgtttttc ccaaagcaga 721 tgcaggagaa cttaaagccc aaacagttcc ggaagatgat ccagcagacc ttccagcagt 781 acgcctcgct cagggaggag gagtgcgtca tgaagttctt caacactctc gccggcttcg 841 ccaacatcga ccaggagacc taccgctgtg aactcattca aggatggaac attactgtgg 901 acctggtcat tggccctaaa gggatccgcc agctgactag tcaggacgca aagcccacct 961 gcctggccga gttcaagcag atcaggtcca tcaggtgcct cccgctggag gagggccagg 1021 cagtacttca gctgggcatt gaaggtgccc cccaggcctt gtccatcaaa acctcatccc 1081 tagcagaggc tgagaacatg gctgacctca tagacggcta ctgccggctg cagggtgagc 1141 accaaggctc tctcatcatc catcctagga aagatggtga gaagcggaac agcctgcccc 1201 agatccccat gctaaacctg gaggcccggc ggtcccacct ctcagagagc tgcagcatag 1261 agtcagacat ctacgcagag attcccgacg aaaccctgcg aaggcccgga ggtccacagt 1321 atggcattgc ccgtgaagat gtggtcctga atcgtattct tggggaaggc ttttttgggg 1381 aggtctatga aggtgtctac acaaatcata aaggggagaa aatcaatgta gctgtcaaga 1441 cctgcaagaa agactgcact ctggacaaca aggagaagtt catgagcgag gcagtgatca 1501 tgaagaacct cgaccacccg cacatcgtga agctgatcgg catcattgaa gaggagccca 1561 cctggatcat catggaattg tatccctatg gggagctggg ccactacctg gagcggaaca 1621 agaactccct gaaggtgctc accctcgtgc tgtactcact gcagatatgc aaagccatgg 1681 cctacctgga gagcatcaac tgcgtgcaca gggacattgc tgtccggaac atcctggtgg 1741 cctcccctga gtgtgtgaag ctgggggact ttggtctttc ccggtacatt gaggacgagg 1801 actattacaa agcctctgtg actcgtctcc ccatcaaatg gatgtcccca gagtccatta 1861 acttccgacg cttcacgaca gccagtgacg tctggatgtt cgccgtgtgc atgtgggaga 1921 tcctgagctt tgggaagcag cccttcttct ggctggagaa caaggatgtc atcggggtgc 1981 tggagaaagg agaccggctg cccaagcctg atctctgtcc accggtcctt tataccctca 2041 tgacccgctg ctgggactac gaccccagtg accggccccg cttcaccgag ctggtgtgca 2101 gcctcagtga cgtttatcag atggagaagg acattgccat ggagcaagag aggaatgctc 2161 gctaccgaac ccccaaaatc ttggagccca cagccttcca ggaaccccca cccaagccca 2221 gccgacctaa gtacagaccc cctccgcaaa ccaacctcct ggctccaaag ctgcagttcc 2281 aggttcctga gggtctgtgt gccagctctc ctacgctcac cagccctatg gagtatccat 2341 ctcccgttaa ctcactgcac accccacctc tccaccggca caatgtcttc aaacgccaca 2401 gcatgcggga ggaggacttc atccaaccca gcagccgaga agaggcccag cagctgtggg 2461 aggctgaaaa ggtcaaaatg cggcaaatcc tggacaaaca gcagaagcag atggtggagg 2521 actaccagtg gctcaggcag gaggagaagt ccctggaccc catggtttat atgaatgata 2581 agtccccatt gacgccagag aaggaggtcg gctacctgga gttcacaggg cccccacaga 2641 agcccccgag gctgggcgca cagtccatcc agcccacagc taacctggac cGGACCGATG 2701 ACCTGGTGTA CCTCAATGTC ATGGAGCTGG TGCGGGCCGT GCTGGAGCTC AAGAATGAGC 2761 TCTGTCAGCT GCCCCCCGAG GGCTACGTGG TGGTGGTGAA GAATGTGGGG CTGACCCTGC 2821 GGAAGCTCAT CGGGAGCGTG GATGATCTCC TGCCTTCCTT GCCGTCATCT TCACGGACAG 2881 AGATCGAGGG CACCCAGAAA CTGCTCAACA AAGACCTGGC AGAGCTCATC AACAAGATGC 2941 GGCTGGCGCA GCAGAACGCC GTGACCTCCC TGAGTGAGGA GTGCAAGAGG CAGATGCTGA 3001 CGGCTTCACA CACCCTGGCT ATGGACGCCA AGAACCTGCT CGACGCTGTG GACCAGGCCA 3061 AGGTTCTGGC CAATCTGGCC CACCCACCTG CAGAGTAGAA CCCAGCTTTC TTGTACAAAG 3121 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 3181 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 3241 ACGACGTATT CTTTATGGAT GTCTAACCAC GCGTTAAGTC gacaatcaac ctctggatta 3301 caaaatttgt gaaagatt