Construct: ORF TRCN0000489606
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021223.1_s317c1
- DNA Barcode:
- GCAACAGGTGTCCTTTCTATCTGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADGRF3 (165082)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489606
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 165082 | ADGRF3 | adhesion G protein-coupled ... | NM_001145168.1 | 100% | 100% | |
| 2 | human | 165082 | ADGRF3 | adhesion G protein-coupled ... | NM_001145169.1 | 91.6% | 91.1% | (many diffs) |
| 3 | human | 165082 | ADGRF3 | adhesion G protein-coupled ... | XM_011532622.2 | 90.3% | 89.3% | (many diffs) |
| 4 | human | 165082 | ADGRF3 | adhesion G protein-coupled ... | NM_001321971.2 | 90.2% | 87.5% | (many diffs) |
| 5 | human | 165082 | ADGRF3 | adhesion G protein-coupled ... | XM_011532621.3 | 90% | 87% | (many diffs) |
| 6 | human | 165082 | ADGRF3 | adhesion G protein-coupled ... | XM_017003505.1 | 89% | 86.2% | (many diffs) |
| 7 | human | 165082 | ADGRF3 | adhesion G protein-coupled ... | XM_011532623.2 | 88.8% | 87.3% | (many diffs) |
| 8 | human | 165082 | ADGRF3 | adhesion G protein-coupled ... | NM_153835.4 | 78.2% | 75.5% | (many diffs) |
| 9 | human | 165082 | ADGRF3 | adhesion G protein-coupled ... | XM_017003506.2 | 75.1% | 74.4% | (many diffs) |
| 10 | human | 165082 | ADGRF3 | adhesion G protein-coupled ... | NR_135916.2 | 62.2% | (many diffs) | |
| 11 | human | 165082 | ADGRF3 | adhesion G protein-coupled ... | NM_001321975.2 | 61.2% | 60.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3309
- ORF length:
- 3237
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggtctgt tcggctgccc cactgctgct cctggccaca actcttcccc 121 tgctggggtc accagttgcc caagcatccc aacctgtaag tgagactggg gtgagaccca 181 gggaaggtct gcagaggcga caatggggac ccctgattgg gagagacaaa gcatggaatg 241 aaaggataga cagacccttc cctgcctgcc ccatccccct atcttctagc tttggccgat 301 ggcccaaggg ccagacaatg tgggcccaga cctccaccct caccctgaca gaggaggagt 361 tgggacagag tcaggctgga ggggaatctg gatctgggca gctcctggac caagagaatg 421 gagcagggga atcagcgctg gtctccgtct atgtacatct ggactttcca gataagacct 481 ggccccctga actctccagg acactgactc tccctgctgc ctcagcttcc tcttccccaa 541 ggcctcttct cactggcctc agactcacaa cagagtgtaa tgtcaaccac aaggggaatt 601 tctattgtgc ttgcctctct ggctaccagt ggaacaccag catctgcctc cattaccctc 661 cttgtcaaag cctccacaac caccagcctt gtggctgcct tgtcttcagc catcccgaac 721 ccgggtactg ccagttgctg ccacctgggt cccctgtcac ctgcctccct gcagtccccg 781 ggatcctcaa cctgaactcc cagctgcaga tgcctggtga cacgctgagc ctgactctcc 841 atctgagcca ggaggccacc aacctgagct ggttcctgag gcacccaggg agccccagtc 901 ccatcctcct gcagccaggg acacaggtgt ctgtgacttc cagccacggc caggctgccc 961 tcagcgtctc caacatgtcc catcactggg caggtgagta catgagctgc ttcgaggccc 1021 agggcttcaa gtggaacctg tatgaggtgg tgagggtgcc cttgaaggcg acagatgtgg 1081 ctcgacttcc ataccagctg tccatctcct gtgccacctc ccctggcttc cagctgagct 1141 gctgcatccc cagcacaaac ctggcctaca ccgcggcctg gagccctgga gagggcagca 1201 aagcttcctc cttcaacgag tcaggctctc agtgctttgt gctggctgtt cagcgctgcc 1261 cgatggctga caccacgtac gcttgtgacc tgcagagcct gggcctggct ccactcaggg 1321 tccccatctc catcaccatc atccaggatg gagacatcac ctgccctgag gacgcctcgg 1381 tgctcacctg gaatgtcacc aaggctggcc acgtggcaca ggccccatgt cctgagagca 1441 agaggggcat agtgaggagg ctctgtgggg ctgacggagt ctgggggccg gtccacagca 1501 gctgcacaga tgcgaggctc ctggccttgt tcactagaac caagctgctg caggcaggcc 1561 agggcagtcc tgctgaggag gtgccacaga tcctggcaca gctgccaggg caggcggcag 1621 aggcaagttc accctccgac ttactgaccc tgctgagcac catgaaatac gtggccaagg 1681 tggtggcaga ggccagaata cagcttgacc gcagagccct gaagaatctc ctgattgcca 1741 cagacaaggt cctagatatg gacaccaggt ctctgtggac cctggcccaa gcccggaagc 1801 cctgggcagg ctcgactctc ctgctggctg tggagaccct ggcatgcagc ctgtgcccac 1861 aggaccaccc cttcgccttc agcttaccca atgtgctgct gcagagccag ctgtttggac 1921 ccacgtttcc tgctgactac agcatctcct tccctactcg gcccccactg caggctcaga 1981 ttcccaggca ctcactggcc ccattggtcc gtaatggaac tgaaataagt attactagcc 2041 tggtgctgcg aaaactggac caccttctgc cctcaaacta tggacaaggg ctgggggatt 2101 ccctctatgc cactcctggc ctggtccttg tcatttccat catggcaggt gaccgggcct 2161 tcagccaggg agaggtcatc atggactttg ggaacacaga tggttcccct cactgtgtct 2221 tctgggatca cagtctcttc cagggcaggg ggggttggtc caaagaaggg tgccaggcac 2281 aggtggccag tgccagcccc actgctcagt gcctctgcca gcacctcact gccttctccg 2341 tcctcatgtc cccacacact gttccggaag aacccgctct ggcgctgctg actcaagtgg 2401 gcttgggagc ttccatactg gcgctgcttg tgtgcctggg tgtgtactgg ctggtgtgga 2461 gagtcgtggt gcggaacaag atctcctatt tccgccacgc cgccctgctc aacatggtgt 2521 tctgcttgct ggccgcagac acttgcttcc tgggcgcccc attcctctct ccagggcccc 2581 gaagcccgct ctgccttgct gccgccttcc tctgtcattt cctctacctg gccacctttt 2641 tctggatgct ggcgcaggcc ctggtgttgg cccaccagct gctctttgtc tttcaccagc 2701 tggcaaagca ccgagttctc cccctcatgg tgctcctggg ctacctgtgc ccactggggt 2761 tggcaggtgt caccctgggg ctctacctac ctcaagggca atacctgagg gagggggaat 2821 gctggttgga tgggaaggga ggggcgttat acaccttcgt ggggccagtg ctggccatca 2881 taggcgtgaa tgggctggta ctagccatgg ccatgctgaa gttgctgaga ccttcgctgt 2941 cagagggacc cccagcagag aagcgccaag ctctgctggg ggtgatcaaa gcccTGCTCA 3001 TTCTTACACC CATCTTTGGC CTCACCTGGG GGCTGGGCCT GGCCACTCTG TTAGAGGAAG 3061 TCTCCACGGT CCCTCATTAC ATCTTCACCA TTCTCAACAC CCTCCAGGGC GTCTTCATCC 3121 TATTGTTTGG TTGCCTCATG GACAGGAAGA TACAAGAAGC TTTGCGCAAA CGCTTCTGCC 3181 GCGCCCAAGC CCCCAGCTCC ACCATCTCCC TGGTGAGTTG CTGCCTTCAG ATCCTCAGCT 3241 GTGCATCCAA GAGCATGTCA GAAGGCATTC CATGGCCCTC CTCAGAGGAC ATGGGCACAG 3301 CCAGAAGCTA AGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 3361 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 3421 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGCA ACAGGTGTCC TTTCTATCTG 3481 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t