Construct: ORF TRCN0000489608
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021205.1_s317c1
- DNA Barcode:
- GCGAAAGTCGAATTCATGGGCTAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GRPR (2925)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489608
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2925 | GRPR | gastrin releasing peptide r... | NM_005314.3 | 99.8% | 100% | 453T>C;663T>C |
| 2 | mouse | 14829 | Grpr | gastrin releasing peptide r... | NM_008177.3 | 87.5% | 89.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1224
- ORF length:
- 1152
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggctcta aatgactgtt tccttctgaa cttggaggtg gaccatttca 121 tgcactgcaa catctccagt cacagtgcgg atctccccgt gaacgatgac tggtcccacc 181 cggggatcct ctatgtcatc cctgcagttt atggggttat cattctgata ggcctcattg 241 gcaacatcac tttgatcaag atcttctgta cagtcaagtc catgcgaaac gttccaaacc 301 tgttcatttc cagtctggct ttgggagacc tgctcctcct aataacgtgt gctccagtgg 361 atgccagcag gtacctggct gacagatggc tatttggcag gattggctgc aaactgatcc 421 cctttataca gcttacctct gttggggtgt ctgtcttcac actcacggcg ctctcggcag 481 acagatacaa agccattgtc cggccaatgg atatccaggc ctcccatgcc ctgatgaaga 541 tctgcctcaa agccgccttt atctggatca tctccatgct gctggccatt ccagaggccg 601 tgttttctga cctccatccc ttccatgagg aaagcaccaa ccagaccttc attagctgtg 661 ccccataccc acactctaat gagcttcacc ccaaaatcca ttctatggct tcctttctgg 721 tcttctacgt catcccactg tcgatcatct ctgtttacta ctacttcatt gctaaaaatc 781 tgatccagag tgcttacaat cttcccgtgg aagggaatat acatgtcaag aagcagattg 841 aaTCCCGGAA GCGACTTGCC AAGACAGTGC TGGTGTTTGT GGGCCTGTTC GCCTTCTGCT 901 GGCTCCCCAA TCATGTCATC TACCTGTACC GCTCCTACCA CTACTCTGAG GTGGACACCT 961 CCATGCTCCA CTTTGTCACC AGCATCTGTG CCCGCCTCCT GGCCTTCACC AACTCCTGCG 1021 TGAACCCCTT TGCCCTCTAC CTGCTGAGCA AGAGTTTCAG GAAACAGTTC AACACTCAGC 1081 TGCTCTGTTG CCAGCCTGGC CTGATCATCC GGTCTCACAG CACTGGAAGG AGTACAACCT 1141 GCATGACCTC CCTCAAGAGT ACCAACCCCT CCGTGGCCAC CTTTAGCCTC ATCAATGGAA 1201 ACATCTGTCA CGAGCGGTAT GTCTAGAACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1261 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1321 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCGAAAGT 1381 CGAATTCATG GGCTATACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1441 aagatt