Construct: ORF TRCN0000489609
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021133.1_s317c1
- DNA Barcode:
- TTACCAAAACCGGGTCTTAGAGGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GPR149 (344758)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489609
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 344758 | GPR149 | G protein-coupled receptor 149 | NM_001038705.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2265
- ORF length:
- 2193
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtcttta tttctcagta acttatcaac aaatgactct agcctgtgga 121 aagagaatca taattctacg gaccttttaa atccgccagg aaccctgaat atctatcttt 181 tttgcttgac atgtctcatg acttttgcag ccttggtggg cagcatttat tcactaattt 241 ccctgctgaa aatgcagaac agaactgttg tgtccatgct tgtggcttcc tggtctgtgg 301 atgatctcat gagcgtcctg tcggtgacca tcttcatgtt tttgcagtgg ccaaacgagg 361 tccccggtta cttccaattt ctgtgcacca cctctgcctt aatgtattta tgccagggcc 421 tctctagcaa cttgaaggcg actctcctag tctcttacaa cttttatacg atgcacagag 481 gtgtggggag ccagacagcc tccagaagat cgggccaggt gctcggcgtg gtgctgaccg 541 tgtgggcagc cagtctgctg ctctcggcgc tcccgctgtg cggctggggc gccttcgtgc 601 gcacgccctg gggctgcctg gtggactgct ccagctccta cgtactattc ctctctatcg 661 tgtacgcttt ggccttcgga ctcctcgtgg gcctctcagt cccactcact caccgattgc 721 tgtgttcgga ggagccgccg agactccact ccaactacca ggaaatttcc cgtggagctt 781 caattcctgg gacccctcct actgcgggga gagtggtttc cctgtcccca gaggatgctc 841 caggcccgag tctgcggcgc tctgggggat gctctccgag ctccgacacc gtgttcggac 901 cgggtgcgcc cgctgccgct ggggctgaag cctgcaggcg tgagaaccgg gggactctct 961 atggcaccag gagcttcacc gtgagcgtag cgcagaagcg cttcgctttg atcctagcgc 1021 ttacaaaagt cgtcctttgg ctgcccatga tgatgcacat ggtggtccag aacgtcgtgg 1081 ggtttcagag ccttcccttg gagacattca gctttctact taccctgctg gccaccactg 1141 taaccccagt gtttgtcttg tccaaacgct ggacccactt gccctgtggc tgcatcatca 1201 actgcaggca gaacgcatat gcagtggcgt ccgatgggaa aaaaatcaag agaaaaggct 1261 ttgaattcaa tctatcattc caaaaaagtt atgggattta taaaatagca catgaagatt 1321 actatgatga tgatgaaaat tccatattct atcacaacct gatgaactct gagtgtgaaa 1381 ctacaaaaga ccctcagaga gacaaccgta acatcttcaa tgctataaaa gtagaaatca 1441 gcaccacgcc ctctctggac agctccacac aaagaggcat caacaaatgc acaaatactg 1501 atattacaga agctaaacag gattccaaca acaaaaagga tgcgttttct gacaaaacag 1561 gaggtgatat taactatgaa gaaactacct tttctgaagg gccagaaaga agactgtctc 1621 atgaagagag tcagaaacca gatctttcag actgggagtg gtgtaggagt aaatcagaaa 1681 gaacccctcg tcagcgttcc ggttatgccc ttgccattcc cttgtgtgca ttccagggga 1741 ctgtgtctct ccatgcacct acagggaaaa ccctatctct ttctacctat gaggtaagcg 1801 cagaagggca aaaaataact ccagcctcta agaaaataga agtctatcga tccaaaagtg 1861 ttggccatga accaaacTCA GAAGATTCTT CATCCACGTT TGTGGACACC AGTGTGAAAA 1921 TACACTTGGA GGTTCTTGAA ATTTGTGATA ATGAAGAGGC CTTGGACACT GTGTCAATCA 1981 TTAGTAACAT CAGTCAGTCC TCCACACAAG TCAGATCTCC ATCCCTACGT TACTCCAGGA 2041 AAGAAAACAG ATTTGTTTCA TGTGACCTAG GGGAAACAGC CTCATACTCC CTCTTTTTGC 2101 CCACCAGTAA TCCTGATGGT GATATTAATA TCTCCATTCC AGACACAGTA GAAGCACACA 2161 GGCAGAACAG TAAAAGGCAG CATCAAGAGA GGGATGGCTA CCAGGAGGAA ATCCAGTTGT 2221 TAAATAAAGC TTACAGAAAA AGAGAGGAAG AAAGCAAGGG TAGTTAGGAC CCAGCTTTCT 2281 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2341 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2401 GTGGAAAGGA CGATTACCAA AACCGGGTCT TAGAGGCACG CGTTAAGTCg acaatcaacc 2461 tctggattac aaaatttgtg aaagatt