Construct: ORF TRCN0000489629
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019373.1_s317c1
- DNA Barcode:
- CCCGAAGCGTAATCTCACGGTACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GBP7 (388646)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489629
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 388646 | GBP7 | guanylate binding protein 7 | NM_207398.3 | 99.9% | 99.8% | 1914_1915insG |
| 2 | human | 115361 | GBP4 | guanylate binding protein 4 | NM_052941.5 | 81.9% | 74% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 59
- ORF end:
- 1976
- ORF length:
- 1917
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaccat 61 ggcatcagag atccacatgc caggcccagt gtgcctcact gagaacacta aagggcatct 121 ggtggtgaat tcagaagctc tggaaatcct gtctgccatt acacagcctg tagtagtggt 181 ggcaattgtg ggcctctacc gcacaggcaa atcctaccta atgaacaagc tggctgggaa 241 gaacaaaggc ttccctctgg gctgcacagt gaagtctgaa accaaaggca tctggatgtg 301 gtgtgtgccc cacccctcca agccaaacca caccctgatc cttctggaca cggagggcct 361 gggtgatatg gaaaagagtg accctaagag tgactcgtgg atctttgccc tggctgtgct 421 tctaagcagc agctttgtct acaacagcat gggcaccatc aaccaccagg ccctggagca 481 gctgcactac gtgactgagc taacagagct aatcagggca aaatcgtgcc ccagacctga 541 tgaagttgag gactccagcg agtttgtgag tttctttcca gactttattt ggactgttcg 601 agattttacc ctggagctga agttagatgg acaccccatc acagaagatg agtacctgga 661 gaatgccttg aagctgattt caggcaagaa tccccaaatc caaaattcta acaagcccag 721 ggagtggatc aggcatttct ttccaaaaca gaagtgcttt gtctttgacc ggccaataaa 781 tgacaaaaaa ctcttactcc atgttgaaga agtacgagaa gaccaactgg atagtaattt 841 ccagatgcaa tcagaaaatt tctgttctta tatcttcacc catgcaaaga ccaagaccct 901 gagagaggga atccttgtca ctggaaaccg gctggggatg ctggtggaga cctacctgga 961 tgccatcaac agtggagcga ctccttgtct ggagaatgca atggcagttc tggcccagtg 1021 tgagaactca gcagccgtgc agagggcagc caaccactac agccagcaga tggcccagca 1081 agtgagattc cccacagaca cactccagga gctgctggac gtgcatgcag tttgtgagag 1141 ggaagccatt gcagtcttca tggagtactc cttcaaagat aaaagccagg aatttcagaa 1201 gaagcttgtg gacaccatgg agaaaaagaa ggaagacttt gtgctgcaga atgaagaggc 1261 atctgccaaa tattgtcagg ctgagcttaa gcggctttca gagctcttga cagaaagtat 1321 ttcaagagga actttctttg ttccgggggg gcacaatatc tacttagaag caaaaaagaa 1381 gattgaacag gactatacac tagtgcccag aaaaggagtt aaggcagacg aggtcctcca 1441 gagcttcctg cagtcacagg tggttataga ggaatccatc ctgcagtcag acaaagccct 1501 cactgctgga gagaaggcca tagcagctaa gcaggctaag aaggaggcag ctgaaaagga 1561 acaggagctg cTAAGACAAA AACAGAAGGA ACAGCAGCAA ATGATGGAGG CTCAAGAGAG 1621 AAGTTTCCAG GAAAACATAG CTCAACTCAA GAAGAAGATG GAGAGGGAAA GGGAAAACTA 1681 TATGAGAGAA CTGAGAAAGA TGTTGAGTCA CAAGATGAAG GTCCTAGAAG AACTGCTTAC 1741 TGAAGGATTT AAAGAGATAT TTGAGTCGTT AAATGAAGAG ATTAATCGAC TGAAAGAACA 1801 AATTGAAGCA GCTGAAAATG AAGAGCCCTC AGTGTTTTCA CAGATTCTTG ATGTGGCTGG 1861 CAGTATATTT ATTGCAGCAC TACCTGGGGC TGCTAAGCTA GTTGATTTAG GAATGAAAAT 1921 TCTTAGCTCA TTATGTAATA GGCTGAGAAA TCCTGGTAAG AAAATTATAA GCGACCCAGC 1981 TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA 2041 TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 2101 ATCTTGTGGA AAGGACGACC CGAAGCGTAA TCTCACGGTA CAACGCGTTA AGTCgacaat 2161 caacctctgg attacaaaat ttgtgaaaga tt