Construct: ORF TRCN0000489645
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020818.1_s317c1
- DNA Barcode:
- GGGAAACAGGCCTTCCCGGAGGCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- FZD4 (8322)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489645
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8322 | FZD4 | frizzled class receptor 4 | NM_012193.4 | 100% | 100% | |
| 2 | mouse | 14366 | Fzd4 | frizzled class receptor 4 | NM_008055.4 | 92.3% | 97% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1683
- ORF length:
- 1611
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcctgg cggggcgcag ggccgagcgt cccgggggcg cccgggggcg 121 tcggtctcag tctggggttg ctcctgcagt tgctgctgct cctggggccg gcgcggggct 181 tcggggacga ggaagagcgg cgctgcgacc ccatccgcat ctccatgtgc cagaacctcg 241 gctacaacgt gaccaagatg cccaacctgg ttgggcacga gctgcagacg gacgccgagc 301 tgcagctgac aactttcaca ccgctcatcc agtacggctg ctccagccag ctgcagttct 361 tcctttgttc tgtttatgtg ccaatgtgca cagagaagat caacatcccc attggcccat 421 gcggcggcat gtgtctttca gtcaagagac gctgtgaacc cgtcctgaag gaatttggat 481 ttgcctggcc agagagtctg aactgcagca aattcccacc acagaacgac cacaaccaca 541 tgtgcatgga agggccaggt gatgaagagg tgcccttacc tcacaaaacc cccatccagc 601 ctggggaaga gtgtcactct gtgggaacca attctgatca gtacatctgg gtgaaaagga 661 gcctgaactg tgtgctcaag tgtggctatg atgctggctt atacagccgc tcagccaagg 721 agttcactga tatctggatg gctgtgtggg ccagcctgtg tttcatctcc actgccttca 781 cagtactgac cttcctgatc gattcttcta ggttttccta ccctgagcgc cccatcatat 841 ttctcagtat gtgctataat atttatagca ttgcttatat tgtcaggctg actgtaggcc 901 gggaaaggat atcctgtgat tttgaagagg cagcagaacc tgttctcatc caagaaggac 961 ttaagaacac aggatgtgca ataattttct tgctgatgta cttttttgga atggccagct 1021 ccatttggtg ggttattctg acactcactt ggtttttggc agcaggactc aaatggggtc 1081 atgaagccat tgaaatgcac agctcttatt tccacattgc agcctgggcc atccccgcag 1141 tgaaaaccat tgtcatcttg attatgagac tggtggatgc agatgaactg actggcttgt 1201 gctatgttgg aaaccaaaat ctcgatgccc tcaccgggtt cgtggtggct cccctcttta 1261 cttatttggt cattggaact ttgttcattg ctgcaggttt ggtggCCTTG TTCAAAATTC 1321 GGTCAAATCT TCAAAAGGAT GGGACAAAGA CAGACAAGTT AGAAAGACTG ATGGTCAAGA 1381 TTGGGGTGTT CTCAGTACTG TACACAGTTC CTGCAACGTG TGTGATTGCC TGTTATTTTT 1441 ATGAAATCTC CAACTGGGCA CTTTTTCGGT ATTCTGCAGA TGATTCCAAC ATGGCTGTTG 1501 AAATGTTGAA AATTTTTATG TCTTTGTTGG TGGGCATCAC TTCAGGCATG TGGATTTGGT 1561 CTGCCAAAAC TCTTCACACG TGGCAGAAGT GTTCCAACAG ATTGGTGAAT TCTGGAAAGG 1621 TAAAGAGAGA GAAGAGAGGA AATGGTTGGG TGAAGCCTGG AAAAGGCAGT GAGACTGTGG 1681 TATAGGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1741 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCTAA CTTGAAAGTA TTTCGATTTC 1801 TTGGCTTTAT ATATCTTGTG GAAAGGACGA GGGAAACAGG CCTTCCCGGA GGCAACGCGT 1861 TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt