Construct: ORF TRCN0000489661
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021920.1_s317c1
- DNA Barcode:
- GGTCGTCCTCGTTCAGTTTAACAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PLCB2 (5330)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489661
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5330 | PLCB2 | phospholipase C beta 2 | NM_004573.3 | 99.9% | 100% | 2776C>A |
| 2 | human | 5330 | PLCB2 | phospholipase C beta 2 | NM_001284297.2 | 99.6% | 99.6% | 1471_1472insCAGGTGAGGGCA;2764C>A |
| 3 | human | 5330 | PLCB2 | phospholipase C beta 2 | XM_017022317.2 | 98.8% | 98.8% | 1697_1735del;2815C>A |
| 4 | human | 5330 | PLCB2 | phospholipase C beta 2 | NM_001284298.2 | 98.7% | 98.7% | 2601_2602ins45;2731C>A |
| 5 | human | 5330 | PLCB2 | phospholipase C beta 2 | XM_017022314.2 | 98.3% | 98.2% | 1697_1735del;2815C>A;3391_3411del |
| 6 | human | 5330 | PLCB2 | phospholipase C beta 2 | XM_024449951.1 | 95.9% | 94.6% | 2776C>A;3114_3201del;3295_3296ins58 |
| 7 | human | 5330 | PLCB2 | phospholipase C beta 2 | XM_024449948.1 | 94.9% | 93.5% | (many diffs) |
| 8 | human | 5330 | PLCB2 | phospholipase C beta 2 | XM_024449949.1 | 94.8% | 93.4% | (many diffs) |
| 9 | human | 5330 | PLCB2 | phospholipase C beta 2 | XM_024449950.1 | 94.6% | 93.1% | (many diffs) |
| 10 | human | 5330 | PLCB2 | phospholipase C beta 2 | XM_024449952.1 | 93.7% | 92.2% | (many diffs) |
| 11 | human | 5330 | PLCB2 | phospholipase C beta 2 | XM_017022319.2 | 93.6% | 93.3% | (many diffs) |
| 12 | human | 5330 | PLCB2 | phospholipase C beta 2 | XR_001751315.2 | 74.7% | (many diffs) | |
| 13 | human | 5330 | PLCB2 | phospholipase C beta 2 | XR_001751316.2 | 73.7% | (many diffs) | |
| 14 | human | 5330 | PLCB2 | phospholipase C beta 2 | XR_001751317.2 | 72.8% | (many diffs) | |
| 15 | human | 5330 | PLCB2 | phospholipase C beta 2 | NM_001284299.2 | 16.7% | 16.3% | (many diffs) |
| 16 | mouse | 18796 | Plcb2 | phospholipase C, beta 2 | NM_177568.2 | 85.5% | 85.9% | (many diffs) |
| 17 | mouse | 18796 | Plcb2 | phospholipase C, beta 2 | NM_001290790.1 | 83.8% | 84.1% | (many diffs) |
| 18 | mouse | 18796 | Plcb2 | phospholipase C, beta 2 | XM_017316357.1 | 81.4% | 81.7% | (many diffs) |
| 19 | mouse | 18796 | Plcb2 | phospholipase C, beta 2 | XM_017316359.1 | 55.2% | 57.1% | (many diffs) |
| 20 | mouse | 18796 | Plcb2 | phospholipase C, beta 2 | XM_006498934.3 | 44.4% | 43.9% | (many diffs) |
| 21 | mouse | 18796 | Plcb2 | phospholipase C, beta 2 | XM_006498935.3 | 33.3% | 34.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3627
- ORF length:
- 3555
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtctctg ctcaaccctg tcctgctgcc ccccaaggtg aaggcctatc 121 tgagccaagg ggagcgcttc atcaaatggg atgatgaaac tacagttgcc tctccagtta 181 tcctccgtgt ggatcctaag ggctactact tatactggac gtatcaaagt aaggagatgg 241 agtttctgga tatcaccagc atccgggata ctcgctttgg gaagtttgcc aagatgccca 301 agagccagaa gctccgggac gtcttcaaca tggactttcc tgataacagt ttcctgctga 361 agacactcac ggtggtgtcc ggcccggaca tggtggacct caccttccac aacttcgtct 421 cctacaagga gaacgtgggc aaggcctggg ctgaggacgt actggcccta gtcaaacatc 481 cgctgacggc caacgcctcc cgcagcacct tcctggacaa gatccttgtg aagctcaaga 541 tgcagctcaa ctctgaaggg aagattccgg tgaagaactt tttccagatg tttcctgctg 601 accgcaagcg ggtggaagct gctctcagtg cctgccacct ccccaaaggc aaaaatgacg 661 ccatcaatcc tgaggacttc ccagaacctg tctacaagag tttcctcatg agcctctgtc 721 ctcggccaga aatagatgag atcttcactt cttaccatgc taaggccaaa ccctacatga 781 cgaaggagca cctgaccaaa ttcatcaacc agaaacagcg ggactcccgg cttaactccc 841 tgctgttccc gccagcacgg cctgaccagg tgcagggcct catcgacaag tatgagccca 901 gtggcatcaa tgcacagagg ggccagctgt cacctgaagg catggtctgg tttctctgtg 961 ggccagagaa cagcgtgctg gcccaggaca agctgctgct ccaccacgac atgacgcagc 1021 cactcaatca ttacttcatc aactcgtccc acaacaccta cctgacagcc ggccagttct 1081 caggcctctc ctcggctgag atgtaccgcc aggtgctgct ctctggctgc cgttgcgtgg 1141 agctagactg ctggaagggg aaaccccctg acgaggagcc cattatcacc catggcttca 1201 ccatgaccac agacatcttc ttcaaagaag caattgaggc tattgcagaa agcgccttta 1261 agacctcccc ctatcccatc atcctgtcgt ttgagaacca tgtggactca ccccgccagc 1321 aggctaagat ggctgagtat tgccggacga tctttgggga tatgctgctc acagagcccc 1381 tggaaaagtt cccactaaaa ccaggtgtcc ccctgcccag ccctgaggat ctcaggggca 1441 agatcctcat caagaacaag aagaaccagt tttctggccc cacctcctcc agtaaggata 1501 ctggtgggga ggctgagggc agcagcccac ccagtgcccc tgcaggtgag ggcacagtgt 1561 gggctggcga ggaagggact gagctggagg aggaggaggt ggaagaggaa gaggaggagg 1621 agtcaggaaa cctggatgaa gaagagatta agaagatgca gtcggatgag ggcacagcgg 1681 gcctggaagt gacggcttat gaggagatgt ccagcctagt caattacatc cagcccacca 1741 agttcgtctc ctttgagttc tctgcccaaa agaaccgaag ttatgtcatc tcgtccttca 1801 cagagctcaa ggcatatgac ctgctctcca aggcctcggt gcagtttgtg gactacaaca 1861 agcgccagat gagccgcatt taccccaagg gaacccgcat ggactcctcc aactacatgc 1921 cccagatgtt ctggaatgct ggatgccaga tggttgccct caacttccag acgatggact 1981 tgcccatgca gcagaacatg gcagtatttg agttcaacgg gcagagcggc tacctcctca 2041 agcatgagtt catgcgccgg ccggacaagc agttcaaccc cttctcagtg gaccgcatcg 2101 acgtggtggt ggccaccacc ctttccatta cggtgatctc tgggcagttc ctgtcagaac 2161 gcagcgtgcg cacctatgta gaagtggagc tgtttggcct tcctggggac cccaagaggc 2221 gctatcgaac taagctgtca cccagtacta actccatcaa tcctgtctgg aaggaggagc 2281 cctttgtctt tgagaagatc ttgatgcctg agctggcctc cctcagagtg gctgtgatgg 2341 aggaaggcaa caagtttctt ggacaccgca tcatccccat caatgcccta aattctgggt 2401 accaccacct gtgcctgcac agtgagagca acatgcccct caccatgcct gcgctcttca 2461 tcttcctgga gatgaaggac tacatacctg gtgcttgggc agatctcact gtggccctcg 2521 ccaaccccat taagttcttc agtgcccatg acacgaagtc tgtgaagctc aaggaggcca 2581 tgggaggtct gcctgagaag cccttcccac tggcgagtcc agttgccagc caggtcaatg 2641 gggcgttggc cccaacgagc aatgggtcac cagcagccag ggccggggcc agggaagagg 2701 ctatgaaaga agctgcggag ccgcggaccg ccagcctgga ggagctccgg gagctaaagg 2761 gcgtggtgaa gctgcagcgg cggcacgaga aggagctgcg agagttggag cggcgcggag 2821 cgcggcgctg ggaggagctg ctgcagaggg gcgcggcgca gctggcggag ctcgggccac 2881 cgggcgtggg gggcgtcggg gcctgcaagc tcggtcccgg caagggctct cgcaagaaga 2941 ggagcctgcc ccgcgaggag agcgccggag ccgcgccggg cgagggccct gagggcgtgg 3001 acgggcgcgt gcgggagctg aaagacaggc tggagctgga gctgctgcgg cagggcgagg 3061 agcagtacga gtgcgttctg aagcgcaagg agcagcacgt ggccgagcaa atctccaaaa 3121 tgatggagct ggccagagag aaacaggcgg cagagctgaa ggccctgaag gagacgtcgg 3181 agaacgacac caaagagatg aagaaaaagc tggagacaaa gagactggag cggaTCCAGG 3241 GCATGACCAA AGTCACCACA GACAAGATGG CCCAGGAGAG GTTGAAGAGA GAGATTAACA 3301 ACTCCCACAT CCAGGAAGTA GTGCAGGTGA TCAAGCAGAT GACGGAGAAC TTGGAGAGGC 3361 ACCAGGAGAA GCTGGAGGAG AAGCAGGCGG CTTGCCTGGA ACAGATACGG GAGATGGAAA 3421 AGCAGTTCCA GAAGGAGGCG CTGGCAGAGT ACGAGGCCAG GATGAAGGGT CTGGAGGCAG 3481 AGGTGAAGGA GTCGGTGAGG GCCTGCCTCA GGACCTGCTT TCCCTCCGAG GCCAAGGACA 3541 AGCCTGAGAG GGCCTGCGAG TGCCCCCCAG AGCTGTGTGA GCAGGACCCA CTCATAGCAA 3601 AGGCAGATGC CCAGGAGAGC CGCCTCTGAG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 3661 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 3721 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGTCG 3781 TCCTCGTTCA GTTTAACAAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 3841 tgaaagatt