Transcript: Mouse XM_017316357.1

PREDICTED: Mus musculus phospholipase C, beta 2 (Plcb2), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Plcb2 (18796)
Length:
7693
CDS:
317..3697

Additional Resources:

NCBI RefSeq record:
XM_017316357.1
NBCI Gene record:
Plcb2 (18796)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017316357.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000097149 GCAGGGATTAGAGACTATCAA pLKO.1 3798 3UTR 100% 5.625 7.875 N Plcb2 n/a
2 TRCN0000097152 CCAGGATAAGAAGTCCGTGAA pLKO.1 2629 CDS 100% 4.050 5.670 N Plcb2 n/a
3 TRCN0000097150 GCTATTACTTATACTGGACAT pLKO.1 282 5UTR 100% 4.050 5.670 N Plcb2 n/a
4 TRCN0000097151 CCTGTCATCCTATCATTTGAA pLKO.1 1355 CDS 100% 5.625 3.938 N Plcb2 n/a
5 TRCN0000028231 CAGCAGAACATGGCACTGTTT pLKO.1 2072 CDS 100% 4.950 3.465 N Plcb2 n/a
6 TRCN0000028286 CCTGGAGATGAAGGACTACAT pLKO.1 2548 CDS 100% 4.950 3.465 N Plcb2 n/a
7 TRCN0000097153 GCCAGAAATCGACGAGATCTT pLKO.1 805 CDS 100% 4.950 3.465 N Plcb2 n/a
8 TRCN0000028299 CCACTCTAAAGCTAAGCCCTA pLKO.1 835 CDS 100% 2.160 1.512 N Plcb2 n/a
9 TRCN0000028272 GACAACCACTTCCTGCTGAAA pLKO.1 422 CDS 100% 4.950 2.970 N Plcb2 n/a
10 TRCN0000028296 CGAAGACATGAGAAGGAGCTT pLKO.1 2861 CDS 100% 2.640 1.584 N Plcb2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017316357.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489661 GGTCGTCCTCGTTCAGTTTAACAA pLX_317 11.3% 81.4% 81.7% V5 (not translated due to prior stop codon) (many diffs) n/a
2 TRCN0000487777 CCCTTACCATTCAAGCTTTAGATT pLX_317 44.4% 10.6% 10.6% V5 (not translated due to prior stop codon) (many diffs) n/a
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