Construct: ORF TRCN0000489662
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021813.1_s317c1
- DNA Barcode:
- CACTTATCCGGTGATTTCCGTTCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- BCL2 (596)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489662
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 596 | BCL2 | BCL2 apoptosis regulator | NM_000633.2 | 99.7% | 100% | 21A>G;189G>C |
| 2 | human | 596 | BCL2 | BCL2 apoptosis regulator | XM_011526135.3 | 85.2% | 79.4% | (many diffs) |
| 3 | human | 596 | BCL2 | BCL2 apoptosis regulator | NM_000657.2 | 82.6% | 82.9% | (many diffs) |
| 4 | human | 596 | BCL2 | BCL2 apoptosis regulator | XM_017025917.2 | 82.3% | 82.4% | (many diffs) |
| 5 | human | 596 | BCL2 | BCL2 apoptosis regulator | XR_935248.3 | 28% | (many diffs) | |
| 6 | mouse | 12043 | Bcl2 | B cell leukemia/lymphoma 2 | NM_009741.5 | 88.3% | 90.7% | (many diffs) |
| 7 | mouse | 12043 | Bcl2 | B cell leukemia/lymphoma 2 | NM_177410.3 | 74% | 74% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 99
- ORF end:
- 816
- ORF length:
- 717
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggccgccccc ttcaccagat ctggaaggat ggcgcacgct gggagaacgg 121 ggtacgataa ccgggagata gtgatgaagt acatccatta taagctgtcg cagaggggct 181 acgagtggga tgcgggagat gtgggcgccg cgcccccggg ggccgccccc gcaccgggca 241 tcttctcctc ccagcccggg cacacgcccc atccagccgc atcccgcgac ccggtcgcca 301 ggacctcgcc gctgcagacc ccggctgccc ccggcgccgc cgcggggcct gcgctcagcc 361 cggtgccacc tgtggtccac ctgaccctcc gccaggccgg cgacgacttc tcccgccgct 421 accgccgcga cTTCGCCGAG ATGTCCAGCC AGCTGCACCT GACGCCCTTC ACCGCGCGGG 481 GACGCTTTGC CACGGTGGTG GAGGAGCTCT TCAGGGACGG GGTGAACTGG GGGAGGATTG 541 TGGCCTTCTT TGAGTTCGGT GGGGTCATGT GTGTGGAGAG CGTCAACCGG GAGATGTCGC 601 CCCTGGTGGA CAACATCGCC CTGTGGATGA CTGAGTACCT GAACCGGCAC CTGCACACCT 661 GGATCCAGGA TAACGGAGGC TGGGATGCCT TTGTGGAACT GTACGGCCCC AGCATGCGGC 721 CTCTGTTTGA TTTCTCCTGG CTGTCTCTGA AGACTCTGCT CAGTTTGGCC CTGGTGGGAG 781 CTTGCATCAC CCTGGGTGCC TATCTGGGCC ACAAGTGAAA GCTTAAGGGT GGGCGCGCCG 841 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 901 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 961 TTTATATATC TTGTGGAAAG GACGACACTT ATCCGGTGAT TTCCGTTCCA CGCGTTAAGT 1021 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt