Construct: ORF TRCN0000489663
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021662.1_s317c1
- DNA Barcode:
- AAACGTGTTAAACCTTAATTCCCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- LDLR (3949)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489663
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3949 | LDLR | low density lipoprotein rec... | NM_000527.5 | 99.9% | 100% | 2232A>G |
| 2 | human | 3949 | LDLR | low density lipoprotein rec... | NM_001195798.2 | 99.7% | 99.7% | 2232A>G;2546_2547insGAGACA |
| 3 | human | 3949 | LDLR | low density lipoprotein rec... | XM_011528010.2 | 96.9% | 96.9% | 2232A>G;2310_2311ins78 |
| 4 | human | 3949 | LDLR | low density lipoprotein rec... | NM_001195799.2 | 95.1% | 95.1% | 190_191ins123;2109A>G |
| 5 | human | 3949 | LDLR | low density lipoprotein rec... | NM_001195800.2 | 80.4% | 80.3% | 313_314ins504;1728A>G |
| 6 | human | 3949 | LDLR | low density lipoprotein rec... | NM_001195803.2 | 79.2% | 79.3% | 313_314ins381;1604_1605ins153;1698A>G |
| 7 | human | 3949 | LDLR | low density lipoprotein rec... | XR_001753685.2 | 47.5% | (many diffs) | |
| 8 | human | 3949 | LDLR | low density lipoprotein rec... | XR_001753686.2 | 46.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2649
- ORF length:
- 2580
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggggccctgg ggctggaaat tgcgctggac cgtcgccttg ctcctcgccg 121 cggcggggac tgcagtgggc gacagatgcg aaagaaacga gttccagtgc caagacggga 181 aatgcatctc ctacaagtgg gtctgcgatg gcagcgctga gtgccaggat ggctctgatg 241 agtcccagga gacgtgcttg tctgtcacct gcaaatccgg ggacttcagc tgtgggggcc 301 gtgtcaaccg ctgcattcct cagttctgga ggtgcgatgg ccaagtggac tgcgacaacg 361 gctcagacga gcaaggctgt ccccccaaga cgtgctccca ggacgagttt cgctgccacg 421 atgggaagtg catctctcgg cagttcgtct gtgactcaga ccgggactgc ttggacggct 481 cagacgaggc ctcctgcccg gtgctcacct gtggtcccgc cagcttccag tgcaacagct 541 ccacctgcat cccccagctg tgggcctgcg acaacgaccc cgactgcgaa gatggctcgg 601 atgagtggcc gcagcgctgt aggggtcttt acgtgttcca aggggacagt agcccctgct 661 cggccttcga gttccactgc ctaagtggcg agtgcatcca ctccagctgg cgctgtgatg 721 gtggccccga ctgcaaggac aaatctgacg aggaaaactg cgctgtggcc acctgtcgcc 781 ctgacgaatt ccagtgctct gatggaaact gcatccatgg cagccggcag tgtgaccggg 841 aatatgactg caaggacatg agcgatgaag ttggctgcgt taatgtgaca ctctgcgagg 901 gacccaacaa gttcaagtgt cacagcggcg aatgcatcac cctggacaaa gtctgcaaca 961 tggctagaga ctgccgggac tggtcagatg aacccatcaa agagtgcggg accaacgaat 1021 gcttggacaa caacggcggc tgttcccacg tctgcaatga ccttaagatc ggctacgagt 1081 gcctgtgccc cgacggcttc cagctggtgg cccagcgaag atgcgaagat atcgatgagt 1141 gtcaggatcc cgacacctgc agccagctct gcgtgaacct ggagggtggc tacaagtgcc 1201 agtgtgagga aggcttccag ctggaccccc acacgaaggc ctgcaaggct gtgggctcca 1261 tcgcctacct cttcttcacc aaccggcacg aggtcaggaa gatgacgctg gaccggagcg 1321 agtacaccag cctcatcccc aacctgagga acgtggtcgc tctggacacg gaggtggcca 1381 gcaatagaat ctactggtct gacctgtccc agagaatgat ctgcagcacc cagcttgaca 1441 gagcccacgg cgtctcttcc tatgacaccg tcatcagcag agacatccag gcccccgacg 1501 ggctggctgt ggactggatc cacagcaaca tctactggac cgactctgtc ctgggcactg 1561 tctctgttgc ggataccaag ggcgtgaaga ggaaaacgtt attcagggag aacggctcca 1621 agccaagggc catcgtggtg gatcctgttc atggcttcat gtactggact gactggggaa 1681 ctcccgccaa gatcaagaaa gggggcctga atggtgtgga catctactcg ctggtgactg 1741 aaaacattca gtggcccaat ggcatcaccc tagatctcct cagtggccgc ctctactggg 1801 ttgactccaa acttcactcc atctcaagca tcgatgtcaa cgggggcaac cggaagacca 1861 tcttggagga tgaaaagagg ctggcccacc ccttctcctt ggccgtcttt gaggacaaag 1921 tattttggac agatatcatc aacgaagcca ttttcagtgc caaccgcctc acaggttccg 1981 atgtcaactt gttggctgaa aacctactgt ccccagagga tatggttctc ttccacaacc 2041 tcacccagcc aagaggagtg aactggtgtg agaggaccac cctgagcaat ggcggctgcc 2101 agtatctgtg cctccctgcc ccgcagatca acccccactc gcccaagttt acctgcgcct 2161 gcccggacgg catgctgctg gccagggaca tgaggagctg cctcacagag gctgaggctg 2221 cagtggccac ccaggagaca tccaccgtca ggctaaaggt cagctccaca gccGTAAGGA 2281 CACAGCACAC AACCACCCGG CCTGTTCCCG ACACCTCCCG GCTGCCTGGG GCCACCCCTG 2341 GGCTCACCAC GGTGGAGATA GTGACAATGT CTCACCAAGC TCTGGGCGAC GTTGCTGGCA 2401 GAGGAAATGA GAAGAAGCCC AGTAGCGTGA GGGCTCTGTC CATTGTCCTC CCCATCGTGC 2461 TCCTCGTCTT CCTTTGCCTG GGGGTCTTCC TTCTATGGAA GAACTGGCGG CTTAAGAACA 2521 TCAACAGCAT CAACTTTGAC AACCCCGTCT ATCAGAAGAC CACAGAGGAT GAGGTCCACA 2581 TTTGCCACAA CCAGGACGGC TACAGCTACC CCTCGAGACA GATGGTCAGT CTGGAGGATG 2641 ACGTGGCGTG AGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 2701 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2761 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAA CGTGTTAAAC CTTAATTCCC 2821 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t