Construct: ORF TRCN0000489680
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF022032.1_s317c1
- DNA Barcode:
- GGATCCCTTTAAATCTGCTAGTAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SLC28A3 (64078)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489680
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64078 | SLC28A3 | solute carrier family 28 me... | NM_001199633.2 | 99.8% | 100% | 267A>G;651T>C;867G>A |
| 2 | human | 64078 | SLC28A3 | solute carrier family 28 me... | NM_022127.3 | 99.8% | 100% | 267A>G;651T>C;867G>A |
| 3 | human | 64078 | SLC28A3 | solute carrier family 28 me... | XM_011518905.2 | 87.9% | 89.4% | (many diffs) |
| 4 | human | 64078 | SLC28A3 | solute carrier family 28 me... | XM_011518906.2 | 87.9% | 89.4% | (many diffs) |
| 5 | human | 64078 | SLC28A3 | solute carrier family 28 me... | XM_011518907.2 | 87.8% | 83.1% | (many diffs) |
| 6 | human | 64078 | SLC28A3 | solute carrier family 28 me... | XR_929832.2 | 69.8% | (many diffs) | |
| 7 | human | 64078 | SLC28A3 | solute carrier family 28 me... | XM_011518908.2 | 68.7% | 67.8% | (many diffs) |
| 8 | human | 64078 | SLC28A3 | solute carrier family 28 me... | XM_011518909.2 | 41.6% | 41.3% | (many diffs) |
| 9 | human | 64078 | SLC28A3 | solute carrier family 28 me... | XM_011518910.2 | 41.6% | 41.3% | (many diffs) |
| 10 | human | 64078 | SLC28A3 | solute carrier family 28 me... | NR_037638.3 | 40.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2142
- ORF length:
- 2073
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggagctgagg agtacagcag cccccagagc tgagggctac agcaacgtgg 121 gcttccagaa tgaagaaaac tttcttgaga acgagaacac atcaggaaac aactcaataa 181 gaagcagagc tgtgcaaagc agggagcaca caaacaccaa acaggatgaa gaacaggtca 241 cagttgagca ggattctcca agaaacagag aacacatgga ggatgatgat gaggagatgc 301 aacaaaaagg gtgtttggaa aggaggtatg acacggtatg tggtttctgt aggaaacaca 361 aaacaactct tcggcacatc atctggggca ttttattagc aggttatctg gttatggtga 421 tttcggcctg tgtgctgaac tttcacagag cccttcctct ttttgtgatc accgtggctg 481 ccatcttctt tgttgtctgg gatcacctga tggccaaata cgaacatcga attgatgaga 541 tgctgtctcc tggcagaagg cttctaaaca gccattggtt ctggctgaag tgggtgatct 601 ggagctccct ggtcctagca gttattttct ggttggcctt tgacactgcc aaattgggtc 661 aacagcagct ggtgtccttc ggtgggctca taatgtacat tgtcctgtta tttctattct 721 ccaagtaccc aaccagagtt tactggagac ctgtcttatg gggaatcggg ctacagtttc 781 ttcttgggct cttgattcta aggactgacc ctggatttat agcttttgat tggttgggca 841 gacaagttca gacttttctg gagtacacag atgctggtgc ttcatttgtc tttggtgaga 901 aatacaaaga ccacttcttt gcatttaagg tcctaccgat cgtggttttc ttcagcactg 961 tgatgtccat gctgtactac ctgggactga tgcagtggat tattagaaag gttggatgga 1021 tcatgctagt tactacggga tcatctccta ttgaatctgt agttgcttct ggcaatatat 1081 ttgttggaca aacggagtct ccactgctgg tccgaccata tttaccttac atcaccaagt 1141 ctgaactcca cgccatcatg accgccgggt tctctaccat tgctggaagc gtgctaggtg 1201 catacatttc ttttggggtt ccatcctccc acttgttaac agcgtcagtt atgtcagcac 1261 ctgcgtcatt ggctgctgct aaactctttt ggcctgagac agaaaaacct aaaataaccc 1321 tcaagaatgc catgaaaatg gaaagtggtg attcagggaa tcttctagaa gctgcaacac 1381 agggagcatc ctcctccatc tccctggtgg ccaacatcgc tgtgaatctg attgccttcc 1441 tggccctgct gtcttttatg aattcagccc tgtcctggtt tggaaacatg tttgactacc 1501 cacagctgag ttttgagcta atctgctcct acatcttcat gcccttttcc ttcatgatgg 1561 gagtggaatg gcaggacagc tttatggttg ccagactcat aggttataag accttcttca 1621 atgaatttgt ggcttatgag cacctctcaa aatggatcca cttgaggaaa gaaggtggac 1681 ccaaatttgt aaacggtgtg cagcaatata tatcaattcg ttctgagata atcgccactt 1741 acgctctctg tggttttgcc aatatcgggt ccctaggaat cgtgatcggc ggactcacat 1801 ccatggctcc TTCCAGAAAG CGTGATATCG CCTCGGGGGC AGTGAGAGCT CTGATTGCGG 1861 GGACCGTGGC CTGCTTCATG ACAGCCTGCA TCGCAGGCAT ACTCTCCAGC ACTCCTGTGG 1921 ACATCAACTG CCATCACGTT TTAGAGAATG CCTTCAACTC CACTTTCCCT GGAAACACAA 1981 CCAAGGTGAT AGCTTGTTGC CAAAGTCTGT TGAGCAGCAC TGTTGCCAAG GGTCCTGGTG 2041 AAGTCATCCC AGGAGGAAAC CACAGTCTGT ATTCTTTGAA GGGCTGCTGC ACATTGTTGA 2101 ATCCATCGAC CTTTAACTGC AATGGGATCT CTAATACATT TTGAGACCCA GCTTTCTTGT 2161 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2221 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2281 GAAAGGACGA GGATCCCTTT AAATCTGCTA GTAAACGCGT TAAGTCgaca atcaacctct 2341 ggattacaaa atttgtgaaa gatt