Construct: ORF TRCN0000489702
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021783.1_s317c1
- DNA Barcode:
- TGCTGCGAAAATAACGCGGCGGCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- HRAS (3265)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489702
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3265 | HRAS | HRas proto-oncogene, GTPase | NM_001130442.2 | 98.6% | 100% | 567_568insTGAAAGCT |
| 2 | human | 3265 | HRAS | HRas proto-oncogene, GTPase | NM_005343.4 | 98.6% | 100% | 567_568insTGAAAGCT |
| 3 | human | 3265 | HRAS | HRas proto-oncogene, GTPase | NM_176795.4 | 84.1% | 80.4% | (many diffs) |
| 4 | human | 3265 | HRAS | HRas proto-oncogene, GTPase | NM_001318054.2 | 37.7% | 26.9% | 0_1ins319;132_213del;330_331insTGAAAGCT |
| 5 | mouse | 15461 | Hras | Harvey rat sarcoma virus on... | NM_001130443.1 | 86.4% | 100% | (many diffs) |
| 6 | mouse | 15461 | Hras | Harvey rat sarcoma virus on... | NM_008284.2 | 86.4% | 100% | (many diffs) |
| 7 | mouse | 15461 | Hras | Harvey rat sarcoma virus on... | XM_006536159.2 | 86.4% | 100% | (many diffs) |
| 8 | mouse | 15461 | Hras | Harvey rat sarcoma virus on... | NM_001130444.1 | 73.5% | 79.3% | (many diffs) |
| 9 | mouse | 15461 | Hras | Harvey rat sarcoma virus on... | XR_389950.2 | 45.1% | (many diffs) | |
| 10 | mouse | 15461 | Hras | Harvey rat sarcoma virus on... | XR_389951.2 | 43.6% | (many diffs) | |
| 11 | mouse | 15461 | Hras | Harvey rat sarcoma virus on... | XR_389952.2 | 41.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 657
- ORF length:
- 567
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccgga tccggagcga tgacggaata taagctggtg gtggtgggcg 121 ccggcggtgt gggcaagagt gcgctgacca tccagctgat ccagaaccat tttgtggacg 181 aatacgaccc cactatagag gattcctacc ggaagcaggt ggtcattgat ggggagacgt 241 gcctgttgga catcctggat accgccggcc aggaggagta cagcgccatg cgggaccagt 301 acatgcgcaC CGGGGAGGGC TTCCTGTGTG TGTTTGCCAT CAACAACACC AAGTCTTTTG 361 AGGACATCCA CCAGTACAGG GAGCAGATCA AACGGGTGAA GGACTCGGAT GACGTGCCCA 421 TGGTGCTGGT GGGGAACAAG TGTGACCTGG CTGCACGCAC TGTGGAATCT CGGCAGGCTC 481 AGGACCTCGC CCGAAGCTAC GGCATCCCCT ACATCGAGAC CTCGGCCAAG ACCCGGCAGG 541 GAGTGGAGGA TGCCTTCTAC ACGTTGGTGC GTGAGATCCG GCAGCACAAG CTGCGGAAGC 601 TGAACCCTCC TGATGAGAGT GGCCCCGGCT GCATGAGCTG CAAGTGTGTG CTCTCCTGAA 661 AGCTTAAGGG TGGGCGCGCC GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 721 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 781 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATGCT GCGAAAATAA 841 CGCGGCGGCC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt