Construct: ORF TRCN0000489709
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020717.1_s317c1
- DNA Barcode:
- TTCAGTACGTTTTACAGTATGTCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SCYL3 (57147)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489709
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | NM_020423.7 | 99.8% | 99.8% | (many diffs) |
| 2 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | XM_017001862.1 | 99.8% | 99.8% | (many diffs) |
| 3 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | XM_017001863.1 | 99.8% | 99.8% | (many diffs) |
| 4 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | NM_181093.3 | 92.5% | 92.5% | (many diffs) |
| 5 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | XM_006711465.1 | 92.5% | 92.5% | (many diffs) |
| 6 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | XM_011509801.1 | 92.5% | 92.5% | (many diffs) |
| 7 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | XM_017001864.1 | 79.1% | 79.2% | (many diffs) |
| 8 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | XM_017001865.1 | 79.1% | 79.2% | (many diffs) |
| 9 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | XM_011509802.1 | 73.4% | 73.4% | (many diffs) |
| 10 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | XM_011509803.1 | 73.4% | 73.4% | (many diffs) |
| 11 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | XR_001737336.2 | 69.8% | (many diffs) | |
| 12 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | XR_001737335.2 | 54.4% | (many diffs) | |
| 13 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | XM_024448565.1 | 44.8% | 44.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2136
- ORF length:
- 2064
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggatca gagaacagtg ctttaaagag ctatacactg agagaaccac 121 catttacctt accctctgga cttgctgttt atcccgctgt actgcaagat ggcaaatttg 181 cttcagtttt tgtgtataag agagaaaatg aagacaaggt taataaagct gccaagcatt 241 tgaagacact tcgtcaccct tgcttgctaa gatttttatc ttgtactgtg gaagcggatg 301 gcattcatct tgtcactgag cgagtacagc ccctggaagt ggctttggaa acattgtctt 361 ctgcagaggt ctgtgctggg atctatgaca tattgctggc tcttatcttc cttcatgaca 421 gaggacacct aacacacaat aatgtctgtt tatcatctgt gtttgtgagt gaagatggac 481 actggaagct aggaggaatg gaaactgttt gtaaagtttc tcaggccaca ccagagtttc 541 tgaggagtat tcagtcaata agagacccag catctatccc tcctgaagag atgtctccag 601 aattcacaac tctcccagag tgtcatggac atgcccggga tgccttttca tttggaacat 661 tggtggaaag tttgctcaca atcttaaatg aacaggtttc agcggatgtt ctctccagct 721 ttcaacagac cttgcactca actttgctga atcccattcc aaaatgtcgg ccagcgctct 781 gcaccttact atctcatgac ttcttcagaa atgattttct ggaagttgtg aatttcttga 841 aaagtttaac attgaagagt gaagaggaga aaacggaatt ctttaaattt ctgctggaca 901 gagtcagctg cttgtcagag gaattgatag cttcaaggtt ggtgcctctt ctgcttaatc 961 agttggtgtt tgcagagcca gtggctgtta agagttttct tccttatctg cttggcccca 1021 aaaaagatca tgcgcaggga gaaactcctt gcttgctctc accagccctg ttccagtcac 1081 gggtgatccc cgtgcttctc cagttgtttg aagttcatga agagcatgtg cggatggtgc 1141 tgctgtctca catcgaggcc tacgtggagc acttcactca ggagcagctg aagaaagtca 1201 tcttgccaca ggttttgctg ggcctgcgtg atactagtga ttccattgtg gcaattactc 1261 tgcatagcct agcagtgctg gtctctctgc ttggaccaga ggtggttgtg ggaggagaac 1321 gaaccaagat cttcaaacgc actgccccaa gttttactaa aaatactgac ctttctctag 1381 aaggtgatcc attttctcag cctattaaat ttcccataaa cggactctca gatgtaaaaa 1441 atacttcgga ggacagtgaa aacttcccat caagttctaa aaagtctgag gagtggcctg 1501 actggagtga acctgaggag cctgaaaatc aaactgtcaa catacagatt tggcctagag 1561 aaccttgtga tgatgtcaag tcccagtgca ctaccttgga tgtggaagag tcatcttggg 1621 atgactgcga gcccagcagc ttagatacta aagtaaaccc aggaggtgga atcactgcta 1681 caaaacctgt tacctcagcg gagcagaagc ctattcctGC TTTGCTTTCA CTCACTGAAG 1741 AGTCTATGCC TTGGAAATCA AGCTTACCCC AAAAGATTAG CCTTGTACAA AGGGGGGATG 1801 ACGCAGACCA AATCGAGCCG CCAAAAGTGT CATCACAAGA AAGGCCCCTT AAGGTTCCAT 1861 CAGAACTTGG TTTAGGAGAG GAATTCACCA TTCAAGTAAA AAAGAAGCCA GTAAAAGATC 1921 CTGAGATGGA TTGGTTTGCT GATATGATCC CAGAAATTAA GCCTTCTGCT GCTTTTCTTA 1981 TATTACCTGA ACTGAGGACA GAAATGGTCC CAAAAAAGGA TGATGTCTCC CCAGTGATGC 2041 AGTTTTCCTC AAAATTTGCT GCAGCAGAAA TTACTGAGGG AGAGGCTGAA GGCTGGGAAG 2101 AAGAAGGGGA GCTGAACTGG GAAGATAATA ACTGGTAGAA CCCAGCTTTC TTGTACAAAG 2161 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2221 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2281 ACGATTCAGT ACGTTTTACA GTATGTCCAC GCGTTAAGTC gacaatcaac ctctggatta 2341 caaaatttgt gaaagatt